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PMC:7291971 / 22531-25169 JSONTXT

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LitCovid-PMC-OGER-BB

Id Subject Object Predicate Lexical cue
T502 39-48 CHEBI:36357;CHEBI:36357 denotes compounds
T503 57-65 SP_10 denotes SARS-CoV
T504 66-71 PR:000000125 denotes nsp14
T505 76-81 PR:O43148 denotes hRNMT
T506 125-132 CHEBI:28748;CHEBI:28748 denotes adenine
T507 133-146 CHEBI:23447;CHEBI:23447 denotes dinucleosides
T508 155-163 SP_10 denotes SARS-CoV
T509 201-210 CHEBI:36357;CHEBI:36357 denotes compounds
T510 303-312 CHEBI:36357;CHEBI:36357 denotes compounds
T511 378-386 SP_10 denotes SARS-CoV
T512 459-472 CHEBI:23447;CHEBI:23447 denotes dinucleosides
T513 526-535 CHEBI:36357;CHEBI:36357 denotes compounds
T514 651-659 SP_10 denotes SARS-CoV
T515 733-743 CHEBI:35222;CHEBI:35222 denotes inhibitors
T516 751-760 CHEBI:36357;CHEBI:36357 denotes compounds
T517 814-822 SP_10 denotes SARS-CoV
T518 1008-1016 SP_10 denotes SARS-CoV
T519 1017-1022 PR:000000125 denotes nsp14
T520 1060-1069 CHEBI:35222;CHEBI:35222 denotes inhibitor
T521 1113-1121 CHEBI:36357;CHEBI:36357 denotes compound
T522 1163-1171 SP_10 denotes SARS-CoV
T523 1275-1280 PR:O43148 denotes hRNMT
T524 1311-1319 CHEBI:33910;CHEBI:33910 denotes chlorine
T525 1320-1324 CHEBI:33250;CHEBI:33250 denotes atom
T526 1350-1361 CHEBI:29785;CHEBI:29785 denotes nitro group
T527 1508-1513 CHEBI:53325;CHEBI:53325 denotes nitro
T528 1551-1556 CHEBI:24433;CHEBI:24433 denotes group
T529 1664-1675 CHEBI:29785;CHEBI:29785 denotes nitro group
T530 1683-1689 CHEBI:30396;CHEBI:30396 denotes phenyl
T531 1779-1787 CHEBI:33910;CHEBI:33910 denotes chlorine
T532 1788-1792 CHEBI:33250;CHEBI:33250 denotes atom
T533 1834-1845 CHEBI:29785;CHEBI:29785 denotes nitro group
T534 1877-1885 SP_10 denotes SARS-CoV
T535 1886-1891 PR:000000125 denotes nsp14
T536 1965-1974 CHEBI:36357;CHEBI:36357 denotes compounds
T537 1978-1986 SP_10 denotes SARS-CoV
T538 1997-2002 SP_6;NCBITaxon:9606 denotes human
T539 2039-2044 PR:000000125 denotes nsp14
T31576 2005-2014 CHEBI:36357;CHEBI:36357 denotes MT activi
T67246 2042-2050 SP_10 denotes 14 IC50a
T8618 2051-2056 PR:000000125 denotes (μM)
T22326 2094-2103 CHEBI:35222;CHEBI:35222 denotes 2 40.6 ± 
T4976 2151-2159 SP_10 denotes 0 10 3.9
T14252 2202-2211 CHEBI:36357;CHEBI:36357 denotes .7 ± 0.5
T88128 2295-2304 CHEBI:35222;CHEBI:35222 denotes 9 n.d a C
T62427 2384-2393 CHEBI:35222;CHEBI:35222 denotes experime
T17572 2433-2441 SP_10 denotes The MTas
T56144 2628-2636 CHEBI:36357;CHEBI:36357 denotes at 50 μ

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T78 125-132 Body_part denotes adenine http://purl.org/sig/ont/fma/fma82774

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
424 125-146 Chemical denotes adenine dinucleosides
425 459-472 Chemical denotes dinucleosides
426 1070-1080 Chemical denotes sinefungin MESH:C006235
427 1241-1251 Chemical denotes sinefungin MESH:C006235
428 1311-1319 Chemical denotes chlorine MESH:D002713
429 1350-1355 Chemical denotes nitro
430 1508-1513 Chemical denotes nitro
431 1664-1669 Chemical denotes nitro
432 1779-1787 Chemical denotes chlorine MESH:D002713
433 1834-1839 Chemical denotes nitro

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T58 57-65 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T59 155-163 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T60 378-386 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T61 651-659 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T62 814-822 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T63 1008-1016 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T64 1163-1171 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T65 1877-1885 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T66 1978-1986 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T67 2030-2038 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T183 19-26 http://purl.obolibrary.org/obo/UBERON_0000473 denotes testing
T184 191-197 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tested
T185 214-215 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T186 275-277 http://purl.obolibrary.org/obo/CLO_0008922 denotes S2
T187 275-277 http://purl.obolibrary.org/obo/CLO_0050052 denotes S2
T188 484-492 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T189 699-707 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T190 770-772 http://purl.obolibrary.org/obo/CLO_0053733 denotes 11
T191 802-810 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T192 1177-1178 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T193 1225-1232 http://purl.obolibrary.org/obo/UBERON_0000473 denotes testing
T194 1260-1266 http://purl.obolibrary.org/obo/CLO_0001658 denotes active
T195 1282-1284 http://purl.obolibrary.org/obo/CLO_0053733 denotes 11
T196 1309-1310 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T197 1348-1349 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T198 1538-1539 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T199 1662-1663 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T200 1720-1728 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T201 1832-1833 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T202 1903-1911 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T203 1958-1964 http://purl.obolibrary.org/obo/CLO_0001658 denotes active
T204 1997-2002 http://purl.obolibrary.org/obo/NCBITaxon_9606 denotes human
T205 2008-2018 http://purl.obolibrary.org/obo/CLO_0001658 denotes activities
T206 2171-2173 http://purl.obolibrary.org/obo/CLO_0053733 denotes 11
T207 2301-2302 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T208 2334-2342 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T209 2398-2399 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T210 2428-2430 http://purl.obolibrary.org/obo/CLO_0053733 denotes 11
T211 2443-2451 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T212 2495-2496 http://purl.obolibrary.org/obo/CLO_0001020 denotes a

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T401 125-132 Chemical denotes adenine http://purl.obolibrary.org/obo/CHEBI_16708
T402 275-277 Chemical denotes S2 http://purl.obolibrary.org/obo/CHEBI_29387
T403 733-743 Chemical denotes inhibitors http://purl.obolibrary.org/obo/CHEBI_35222
T404 1060-1069 Chemical denotes inhibitor http://purl.obolibrary.org/obo/CHEBI_35222
T405 1070-1080 Chemical denotes sinefungin http://purl.obolibrary.org/obo/CHEBI_45453
T406 1241-1251 Chemical denotes sinefungin http://purl.obolibrary.org/obo/CHEBI_45453
T407 1311-1324 Chemical denotes chlorine atom http://purl.obolibrary.org/obo/CHEBI_23116
T408 1311-1319 Chemical denotes chlorine http://purl.obolibrary.org/obo/CHEBI_29310
T409 1320-1324 Chemical denotes atom http://purl.obolibrary.org/obo/CHEBI_33250
T410 1350-1361 Chemical denotes nitro group http://purl.obolibrary.org/obo/CHEBI_29785
T411 1356-1361 Chemical denotes group http://purl.obolibrary.org/obo/CHEBI_24433
T412 1508-1513 Chemical denotes nitro http://purl.obolibrary.org/obo/CHEBI_29785
T413 1551-1556 Chemical denotes group http://purl.obolibrary.org/obo/CHEBI_24433
T414 1664-1675 Chemical denotes nitro group http://purl.obolibrary.org/obo/CHEBI_29785
T415 1670-1675 Chemical denotes group http://purl.obolibrary.org/obo/CHEBI_24433
T416 1683-1689 Chemical denotes phenyl http://purl.obolibrary.org/obo/CHEBI_30396
T417 1779-1792 Chemical denotes chlorine atom http://purl.obolibrary.org/obo/CHEBI_23116
T418 1779-1787 Chemical denotes chlorine http://purl.obolibrary.org/obo/CHEBI_29310
T419 1788-1792 Chemical denotes atom http://purl.obolibrary.org/obo/CHEBI_33250
T420 1834-1845 Chemical denotes nitro group http://purl.obolibrary.org/obo/CHEBI_29785
T421 1840-1845 Chemical denotes group http://purl.obolibrary.org/obo/CHEBI_24433

2_test

Id Subject Object Predicate Lexical cue
32563813-28676301-29105587 1282-1284 28676301 denotes 11
32563813-28676301-29105588 2428-2430 28676301 denotes 11

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T157 0-81 Sentence denotes 2.4 Dose-response testing of selected compounds against SARS-CoV nsp14 and hRNMT
T158 82-279 Sentence denotes To confirm the observed inhibition of some adenine dinucleosides against SARS-CoV nsp14 N7-MTase, we further tested 10 compounds in a dose-response assay (Table 2 , Supporting Information Fig. S2).
T159 280-403 Sentence denotes The selection of these compounds was based on their percentage inhibition higher than 50% against SARS-CoV nsp14 (Table 1).
T160 404-513 Sentence denotes After pre-incubation with increasing concentrations of dinucleosides, the MTase activity was measured by FBA.
T161 514-708 Sentence denotes The IC50 of compounds, deduced from Hill slope equation (Y = 100/(1+((X/IC50)^Hillslope) curve-fitting, ranged from 0.6 μM to 70.4 μM on SARS-CoV nsp14 and 10.9 μM to > 500 μM on hRNMT activity.
T162 709-963 Sentence denotes Among all the potential inhibitors, three compounds 2, 6 and 11 showed the lowest inhibitory activity on SARS-CoV nsp14 with IC50 > 40 μM, the others have IC50 values in the micromolar range except 13 that showed an IC50 value in the submicromolar range.
T163 964-1081 Sentence denotes The potency of the derivative 13 to inhibit SARS-CoV nsp14 was comparable to the broad spectrum inhibitor sinefungin.
T164 1082-1286 Sentence denotes However, it is noteworthy that compound 13 specifically inhibits the N7-MTase of SARS-CoV with a high 413-fold of specificity in dose-response testing whereas sinefungin is also active against hRNMT [11].
T165 1287-1587 Sentence denotes Remarkably 13 bearing a chlorine atom in “para” position and a nitro group in “meta” position displayed the best inhibition which was 6.5–9.5 fold more potent than the derivatives 10 and 12, respectively that contain the nitro in “ortho” position and a lipophilic group CF3 or MeO in “para” position.
T166 1588-1747 Sentence denotes The comparison of IC50 values of 14 and 13 indicates that the addition of a nitro group in the phenyl ring increased the inhibitory activity by 2.5 fold in 13.
T167 1748-1912 Sentence denotes These results confirm that the chlorine atom in “para” position and the presence of a nitro group seem crucial for submicromolar SARS-CoV nsp14 inhibitory activity.
T168 1913-2019 Sentence denotes Table 2 Comparison of IC50 values of 10 most active compounds on SARS-CoV nsp14 and human RNMT activities.
T169 2020-2070 Sentence denotes Compounds SARS-CoV nsp14 IC50a (μM) hRNMTIC50 (μM)
T170 2071-2093 Sentence denotes Sinefunginb 0.36 <0.05
T171 2094-2117 Sentence denotes 2 40.6 ± 3.2 10.9 ± 1.0
T172 2118-2135 Sentence denotes 6 55.5 ± 5.1 >500
T173 2136-2152 Sentence denotes 9 2.6 ± 0.2 >500
T174 2153-2170 Sentence denotes 10 3.9 ± 0.4 >500
T175 2171-2197 Sentence denotes 11 70.4 ± 4.9 169.3 ± 30.4
T176 2198-2223 Sentence denotes 12 5.7 ± 0.5 275.9 ± 28.7
T177 2224-2249 Sentence denotes 13 0.6 ± 0.1 247.5 ± 14.9
T178 2250-2266 Sentence denotes 14 1.5 ± 0.1 n.d
T179 2267-2283 Sentence denotes 15 2.4 ± 0.2 n.i
T180 2284-2300 Sentence denotes 16 9.9 ± 0.9 n.d
T181 2301-2397 Sentence denotes a Concentration inhibiting MTase activity by 50%; mean value from three independent experiments.
T182 2398-2432 Sentence denotes b values from the literature [11].
T183 2433-2638 Sentence denotes The MTase activity for IC50 determinations was measured using a filter binding assay as described above. n.i: no inhibition detected at 50 μM; n.d: not determined due to inhibition lower than 50% at 50 μM.

MyTest

Id Subject Object Predicate Lexical cue
32563813-28676301-29105587 1282-1284 28676301 denotes 11
32563813-28676301-29105588 2428-2430 28676301 denotes 11