PMC:7281900 / 1205-2024 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7281900","sourcedb":"PMC","sourceid":"7281900","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7281900","text":"Differentiated NPCs revealed enlarged cell size in TSC1-Het and Null NPCs, consistent with mTORC1 activation. TSC1-Het and Null NPCs also revealed enhanced proliferation and altered neurite outgrowth in a genotype-dependent manner, which was not reversed by rapamycin. Transcriptome analyses of TSC1-NPCs revealed differentially expressed genes that display a genotype-dependent linear response, that is, genes upregulated/downregulated in Het were further increased/decreased in Null. In particular, genes linked to ASD, epilepsy, and ID were significantly upregulated or downregulated, warranting further investigation. In TSC1-Het and Null NPCs, we also observed basal activation of ERK1/2, which was further activated upon rapamycin treatment. Rapamycin also increased MNK1/2-eIF4E signaling in TSC1-deficient NPCs.","tracks":[{"project":"testtesttest","denotations":[{"id":"T4","span":{"begin":38,"end":42},"obj":"Body_part"},{"id":"T5","span":{"begin":156,"end":169},"obj":"Body_part"},{"id":"T6","span":{"begin":182,"end":189},"obj":"Body_part"}],"attributes":[{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL_0000000"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_2000098"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/GO_0043005"},{"subj":"T4","pred":"source","obj":"testtesttest"},{"subj":"T5","pred":"source","obj":"testtesttest"},{"subj":"T6","pred":"source","obj":"testtesttest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"testtesttest","color":"#93ec9c","default":true}]}]}}