PMC:7255289 / 6830-7515
Annnotations
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 58 | 16-23 | Species | denotes | E. coli | Tax:562 |
| 59 | 52-60 | Chemical | denotes | LB broth |
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T3 | 9-14 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T4 | 140-145 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T5 | 368-372 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T6 | 595-602 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
LitCovid-PD-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 246-250 | Body_part | denotes | pore | http://purl.obolibrary.org/obo/UBERON_0008915 |
| T2 | 325-329 | Body_part | denotes | pore | http://purl.obolibrary.org/obo/UBERON_0008915 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T15 | 111-113 | Disease | denotes | OD | http://purl.obolibrary.org/obo/MONDO_0017178 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T45 | 0-8 | http://purl.obolibrary.org/obo/NCBITaxon_2 | denotes | Bacteria |
| T46 | 9-14 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T47 | 131-145 | http://purl.obolibrary.org/obo/CL_0000010 | denotes | cultured cells |
| T48 | 193-197 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
| T49 | 236-237 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T50 | 315-316 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T51 | 356-364 | http://purl.obolibrary.org/obo/NCBITaxon_2 | denotes | bacteria |
| T52 | 368-372 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T29 | 595-602 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T41 | 0-126 | Sentence | denotes | Bacteria cells (E. coli) were cultured overnight in LB broth at 37 °C with shaking (180 rpm) until the desired OD was reached. |
| T42 | 127-198 | Sentence | denotes | The cultured cells were pelleted twice at 5,000 x g for 20 min at 4 °C. |
| T43 | 199-263 | Sentence | denotes | The supernatant was filtered through a 0.45 μm pore size filter. |
| T44 | 264-380 | Sentence | denotes | This supernatant was then further filtered through a 0.22 μm pore size filter to remove any bacteria or cell debris. |
| T45 | 381-533 | Sentence | denotes | The OMVs were purified from the filtered supernatant using ExoBacteria OMV Isolation Kits (SBI, Palo Alto, CA) according to the manufacturer's protocol. |
| T46 | 534-628 | Sentence | denotes | Bradford assay (Bio-Rad, Hercules, CA) was used to determine protein content of isolated OMVs. |
| T47 | 629-685 | Sentence | denotes | The sample was aliquoted and stored at −80 °C until use. |