PMC:7253482 / 27672-28289 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7253482","sourcedb":"PMC","sourceid":"7253482","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7253482","text":"Release and labelling of N-linked glycans\nExcised coronavirus S gel bands were washed alternately with acetonitrile and water before drying in a vacuum centrifuge. The bands were rehydrated with 100 μL of water and incubated with PNGase F at 37 °C overnight. Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied 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