PMC:7252096 / 99046-99342
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7252096","sourcedb":"PMC","sourceid":"7252096","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7252096","text":"Briefly, RNA from population lysates was purified using AMPure RNA Clean Spri beads (Beckman Coulter) at a 2.2x volume ratio, and mixed with oligo-dT primer, dNTPs (NEB), and RNase inhibitor (Fisher Scientific) at 72°C for 3 min on a thermal cycler to anneal the 3′ primer to polyadenylated mRNA.","tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T2","span":{"begin":9,"end":12},"obj":"Body_part"},{"id":"T3","span":{"begin":63,"end":66},"obj":"Body_part"},{"id":"T4","span":{"begin":291,"end":295},"obj":"Body_part"}],"attributes":[{"id":"A2","pred":"fma_id","subj":"T2","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A3","pred":"fma_id","subj":"T3","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A4","pred":"fma_id","subj":"T4","obj":"http://purl.org/sig/ont/fma/fma67122"},{"subj":"T2","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T3","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T4","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T2","span":{"begin":105,"end":106},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T3","span":{"begin":232,"end":233},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"attributes":[{"subj":"T2","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T3","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T412","span":{"begin":181,"end":190},"obj":"Chemical"}],"attributes":[{"id":"A90879","pred":"chebi_id","subj":"T412","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"subj":"T412","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T713","span":{"begin":0,"end":296},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T713","pred":"source","obj":"LitCovid-sentences"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"2627","span":{"begin":158,"end":163},"obj":"Chemical"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"attributes":[{"subj":"2627","pred":"source","obj":"LitCovid-PubTator"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#93e9ec","default":true},{"id":"LitCovid-PD-CLO","color":"#ecd493"},{"id":"LitCovid-PD-CHEBI","color":"#ba93ec"},{"id":"LitCovid-sentences","color":"#93eca0"},{"id":"LitCovid-PubTator","color":"#ec939f"}]}]}}