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PMC:7252096 / 97020-97588 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T1079 67-69 Body_part denotes v1 http://purl.org/sig/ont/fma/fma13444|http://purl.org/sig/ont/fma/fma68614

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T1591 345-347 http://purl.obolibrary.org/obo/CLO_0009287 denotes TE
T1592 435-436 http://purl.obolibrary.org/obo/CLO_0001020 denotes a

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T64977 9-11 Chemical denotes S3 http://purl.obolibrary.org/obo/CHEBI_29388
T79129 21-23 Chemical denotes S3 http://purl.obolibrary.org/obo/CHEBI_29388
T9601 121-127 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T7108 157-176 Chemical denotes polyethylene glycol http://purl.obolibrary.org/obo/CHEBI_46793
T23785 157-169 Chemical denotes polyethylene http://purl.obolibrary.org/obo/CHEBI_53226|http://purl.obolibrary.org/obo/CHEBI_53227|http://purl.obolibrary.org/obo/CHEBI_60034
T40008 170-176 Chemical denotes glycol http://purl.obolibrary.org/obo/CHEBI_13643
T37602 178-181 Chemical denotes PEG http://purl.obolibrary.org/obo/CHEBI_46793
T47327 283-287 Chemical denotes NaOH http://purl.obolibrary.org/obo/CHEBI_32145
T51634 345-347 Chemical denotes TE http://purl.obolibrary.org/obo/CHEBI_74857
T68917 489-492 Chemical denotes PEG http://purl.obolibrary.org/obo/CHEBI_46793
T99070 502-504 Chemical denotes dN http://purl.obolibrary.org/obo/CHEBI_33793
T16126 510-525 Chemical denotes oligonucleotide http://purl.obolibrary.org/obo/CHEBI_7754

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T81 217-226 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion
T82 384-393 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T83 459-474 http://purl.obolibrary.org/obo/GO_0003887 denotes Klenow Fragment

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T694 0-11 Sentence denotes Seq-Well S3
T695 12-99 Sentence denotes Seq-Well S3 modified the following protocol steps from v1, above (Hughes et al., 2019).
T696 100-190 Sentence denotes First, hybridization buffer was supplanted with 8% (v/v) polyethylene glycol (PEG, Sigma).
T697 191-315 Sentence denotes Second, after exonuclease digestion, bead-associated cDNA was denatured for 5 min in 0.2 mM NaOH with end over end rotation.
T698 316-568 Sentence denotes Next, beads were washed with TE + 0.01% tween-20, and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs, PEG, and the dN-SMRT oligonucleotide to enable random priming off of the beads.

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
2608 505-509 Gene denotes SMRT Gene:9612
2609 157-176 Chemical denotes polyethylene glycol MESH:D011092
2610 178-181 Chemical denotes PEG MESH:D011092
2611 283-287 Chemical denotes NaOH
2612 356-364 Chemical denotes tween-20 MESH:D011136
2613 482-487 Chemical denotes dNTPs
2614 489-492 Chemical denotes PEG MESH:D011092
2615 510-525 Chemical denotes oligonucleotide MESH:D009841