PMC:7252096 / 87718-88387 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T991","span":{"begin":4,"end":15},"obj":"Body_part"},{"id":"T992","span":{"begin":4,"end":9},"obj":"Body_part"},{"id":"T993","span":{"begin":17,"end":23},"obj":"Body_part"},{"id":"T994","span":{"begin":29,"end":40},"obj":"Body_part"},{"id":"T995","span":{"begin":184,"end":189},"obj":"Body_part"},{"id":"T996","span":{"begin":259,"end":263},"obj":"Body_part"},{"id":"T997","span":{"begin":276,"end":282},"obj":"Body_part"},{"id":"T998","span":{"begin":284,"end":295},"obj":"Body_part"},{"id":"T999","span":{"begin":407,"end":418},"obj":"Body_part"},{"id":"T1000","span":{"begin":427,"end":432},"obj":"Body_part"},{"id":"T1001","span":{"begin":557,"end":562},"obj":"Body_part"},{"id":"T1002","span":{"begin":654,"end":656},"obj":"Body_part"}],"attributes":[{"id":"A991","pred":"fma_id","subj":"T991","obj":"http://purl.org/sig/ont/fma/fma5034"},{"id":"A992","pred":"fma_id","subj":"T992","obj":"http://purl.org/sig/ont/fma/fma9671"},{"id":"A993","pred":"fma_id","subj":"T993","obj":"http://purl.org/sig/ont/fma/fma7196"},{"id":"A994","pred":"fma_id","subj":"T994","obj":"http://purl.org/sig/ont/fma/fma9608"},{"id":"A995","pred":"fma_id","subj":"T995","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A996","pred":"fma_id","subj":"T996","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A997","pred":"fma_id","subj":"T997","obj":"http://purl.org/sig/ont/fma/fma7196"},{"id":"A998","pred":"fma_id","subj":"T998","obj":"http://purl.org/sig/ont/fma/fma9608"},{"id":"A999","pred":"fma_id","subj":"T999","obj":"http://purl.org/sig/ont/fma/fma9608"},{"id":"A1000","pred":"fma_id","subj":"T1000","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A1001","pred":"fma_id","subj":"T1001","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A1002","pred":"fma_id","subj":"T1002","obj":"http://purl.org/sig/ont/fma/fma13444"},{"id":"A1003","pred":"fma_id","subj":"T1002","obj":"http://purl.org/sig/ont/fma/fma68614"}],"text":"All lymph nodes, spleen, and bone marrow were ground through a metal strainer, transferred to a conical in RPMI + 10% FBS, and pelleted by centrifugation at 400 g x 10 min. LN-derived cells were resuspended in RPMI + 10% FBS, counted and prepared as a single cell suspension. Spleen, bone marrow, and PBMCs were subjected to ACK lysis for 10 min at room temperature, quenched with RPMI + 10% FBS. PBMCs and bone marrow derived cells were purified over a ficoll gradient (GE Healthcare) by centrifuging at 400 g for 20 min at room temperature with no brake. Cells were then resuspended in RPMI + 10% FBS, counted, and diluted for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T193","span":{"begin":4,"end":15},"obj":"Body_part"},{"id":"T194","span":{"begin":4,"end":9},"obj":"Body_part"},{"id":"T195","span":{"begin":17,"end":23},"obj":"Body_part"},{"id":"T196","span":{"begin":29,"end":40},"obj":"Body_part"},{"id":"T197","span":{"begin":276,"end":282},"obj":"Body_part"},{"id":"T198","span":{"begin":284,"end":295},"obj":"Body_part"},{"id":"T199","span":{"begin":407,"end":418},"obj":"Body_part"}],"attributes":[{"id":"A193","pred":"uberon_id","subj":"T193","obj":"http://purl.obolibrary.org/obo/UBERON_0000029"},{"id":"A194","pred":"uberon_id","subj":"T194","obj":"http://purl.obolibrary.org/obo/UBERON_0002391"},{"id":"A195","pred":"uberon_id","subj":"T195","obj":"http://purl.obolibrary.org/obo/UBERON_0002106"},{"id":"A196","pred":"uberon_id","subj":"T196","obj":"http://purl.obolibrary.org/obo/UBERON_0002371"},{"id":"A197","pred":"uberon_id","subj":"T197","obj":"http://purl.obolibrary.org/obo/UBERON_0002106"},{"id":"A198","pred":"uberon_id","subj":"T198","obj":"http://purl.obolibrary.org/obo/UBERON_0002371"},{"id":"A199","pred":"uberon_id","subj":"T199","obj":"http://purl.obolibrary.org/obo/UBERON_0002371"}],"text":"All lymph nodes, spleen, and bone marrow were ground through a metal strainer, transferred to a conical in RPMI + 10% FBS, and pelleted by centrifugation at 400 g x 10 min. LN-derived cells were resuspended in RPMI + 10% FBS, counted and prepared as a single cell suspension. Spleen, bone marrow, and PBMCs were subjected to ACK lysis for 10 min at room temperature, quenched with RPMI + 10% FBS. PBMCs and bone marrow derived cells were purified over a ficoll gradient (GE Healthcare) by centrifuging at 400 g for 20 min at room temperature with no brake. Cells were then resuspended in RPMI + 10% FBS, counted, and diluted for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T350","span":{"begin":173,"end":175},"obj":"Disease"}],"attributes":[{"id":"A350","pred":"mondo_id","subj":"T350","obj":"http://purl.obolibrary.org/obo/MONDO_0002486"}],"text":"All lymph nodes, spleen, and bone marrow were ground through a metal strainer, transferred to a conical in RPMI + 10% FBS, and pelleted by centrifugation at 400 g x 10 min. LN-derived cells were resuspended in RPMI + 10% FBS, counted and prepared as a single cell suspension. Spleen, bone marrow, and PBMCs were subjected to ACK lysis for 10 min at room temperature, quenched with RPMI + 10% FBS. PBMCs and bone marrow derived cells were purified over a ficoll gradient (GE Healthcare) by centrifuging at 400 g for 20 min at room temperature with no brake. Cells were then resuspended in RPMI + 10% FBS, counted, and diluted for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1456","span":{"begin":4,"end":15},"obj":"http://purl.obolibrary.org/obo/UBERON_0000029"},{"id":"T1457","span":{"begin":17,"end":23},"obj":"http://purl.obolibrary.org/obo/UBERON_0002106"},{"id":"T1458","span":{"begin":29,"end":33},"obj":"http://purl.obolibrary.org/obo/UBERON_0002481"},{"id":"T1459","span":{"begin":61,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T1460","span":{"begin":94,"end":95},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T1461","span":{"begin":184,"end":189},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1462","span":{"begin":250,"end":251},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T1463","span":{"begin":259,"end":263},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1464","span":{"begin":276,"end":282},"obj":"http://purl.obolibrary.org/obo/UBERON_0002106"},{"id":"T1465","span":{"begin":284,"end":288},"obj":"http://purl.obolibrary.org/obo/UBERON_0002481"},{"id":"T1466","span":{"begin":407,"end":411},"obj":"http://purl.obolibrary.org/obo/UBERON_0002481"},{"id":"T1467","span":{"begin":427,"end":432},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1468","span":{"begin":452,"end":453},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T1469","span":{"begin":557,"end":562},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"All lymph nodes, spleen, and bone marrow were ground through a metal strainer, transferred to a conical in RPMI + 10% FBS, and pelleted by centrifugation at 400 g x 10 min. LN-derived cells were resuspended in RPMI + 10% FBS, counted and prepared as a single cell suspension. Spleen, bone marrow, and PBMCs were subjected to ACK lysis for 10 min at room temperature, quenched with RPMI + 10% FBS. PBMCs and bone marrow derived cells were purified over a ficoll gradient (GE Healthcare) by centrifuging at 400 g for 20 min at room temperature with no brake. Cells were then resuspended in RPMI + 10% FBS, counted, and diluted for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T37446","span":{"begin":173,"end":175},"obj":"Chemical"},{"id":"T49328","span":{"begin":471,"end":473},"obj":"Chemical"}],"attributes":[{"id":"A98409","pred":"chebi_id","subj":"T37446","obj":"http://purl.obolibrary.org/obo/CHEBI_17571"},{"id":"A67265","pred":"chebi_id","subj":"T37446","obj":"http://purl.obolibrary.org/obo/CHEBI_73529"},{"id":"A52471","pred":"chebi_id","subj":"T49328","obj":"http://purl.obolibrary.org/obo/CHEBI_73801"}],"text":"All lymph nodes, spleen, and bone marrow were ground through a metal strainer, transferred to a conical in RPMI + 10% FBS, and pelleted by centrifugation at 400 g x 10 min. LN-derived cells were resuspended in RPMI + 10% FBS, counted and prepared as a single cell suspension. Spleen, bone marrow, and PBMCs were subjected to ACK lysis for 10 min at room temperature, quenched with RPMI + 10% FBS. PBMCs and bone marrow derived cells were purified over a ficoll gradient (GE Healthcare) by centrifuging at 400 g for 20 min at room temperature with no brake. Cells were then resuspended in RPMI + 10% FBS, counted, and diluted for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T57","span":{"begin":329,"end":334},"obj":"http://purl.obolibrary.org/obo/GO_0019835"}],"text":"All lymph nodes, spleen, and bone marrow were ground through a metal strainer, transferred to a conical in RPMI + 10% FBS, and pelleted by centrifugation at 400 g x 10 min. LN-derived cells were resuspended in RPMI + 10% FBS, counted and prepared as a single cell suspension. Spleen, bone marrow, and PBMCs were subjected to ACK lysis for 10 min at room temperature, quenched with RPMI + 10% FBS. PBMCs and bone marrow derived cells were purified over a ficoll gradient (GE Healthcare) by centrifuging at 400 g for 20 min at room temperature with no brake. Cells were then resuspended in RPMI + 10% FBS, counted, and diluted for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T624","span":{"begin":0,"end":172},"obj":"Sentence"},{"id":"T625","span":{"begin":173,"end":275},"obj":"Sentence"},{"id":"T626","span":{"begin":276,"end":396},"obj":"Sentence"},{"id":"T627","span":{"begin":397,"end":556},"obj":"Sentence"},{"id":"T628","span":{"begin":557,"end":669},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"All lymph nodes, spleen, and bone marrow were ground through a metal strainer, transferred to a conical in RPMI + 10% FBS, and pelleted by centrifugation at 400 g x 10 min. LN-derived cells were resuspended in RPMI + 10% FBS, counted and prepared as a single cell suspension. Spleen, bone marrow, and PBMCs were subjected to ACK lysis for 10 min at room temperature, quenched with RPMI + 10% FBS. PBMCs and bone marrow derived cells were purified over a ficoll gradient (GE Healthcare) by centrifuging at 400 g for 20 min at room temperature with no brake. Cells were then resuspended in RPMI + 10% FBS, counted, and diluted for scRNA-seq using Seq-Well v1 (see below)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"2439","span":{"begin":325,"end":328},"obj":"Gene"},{"id":"2440","span":{"begin":107,"end":111},"obj":"Chemical"},{"id":"2441","span":{"begin":210,"end":214},"obj":"Chemical"},{"id":"2442","span":{"begin":381,"end":385},"obj":"Chemical"},{"id":"2443","span":{"begin":588,"end":592},"obj":"Chemical"}],"attributes":[{"id":"A2439","pred":"tao:has_database_id","subj":"2439","obj":"Gene:10188"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"All lymph nodes, spleen, and bone marrow were ground through a metal strainer, transferred to a conical in RPMI + 10% FBS, and pelleted by centrifugation at 400 g x 10 min. LN-derived cells were resuspended in RPMI + 10% FBS, counted and prepared as a single cell suspension. Spleen, bone marrow, and PBMCs were subjected to ACK lysis for 10 min at room temperature, quenched with RPMI + 10% FBS. PBMCs and bone marrow derived cells were purified over a ficoll gradient (GE Healthcare) by centrifuging at 400 g for 20 min at room temperature with no brake. Cells were then resuspended in RPMI + 10% FBS, counted, and diluted for scRNA-seq using Seq-Well v1 (see below)."}