PMC:7247521 / 18071-20556 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7247521","sourcedb":"PMC","sourceid":"7247521","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7247521","text":"3.2 Functional and pathway enrichment analyses of QFPD targets\nAs shown in Fig. 2 , the 11 enriched GO terms of the targets in all five formulae were found, such as oxidoreductase activity, lipid metabolic process, lipid binding, small molecule metabolic process, homeostatic process, signal transducer activity and cell proliferation. Furthermore, the results of pathway enrichment analysis showed that the 7 KEGG pathways were significantly related to more than 4 formula groups, including dteroid biosynthesis, adipocytokine signaling pathway, neuroactive ligand-receptor interaction, steroid hormone biosynthesis, PPAR signaling pathway, arginine and proline metabolism and ABC transporters (Fig. 3 A). In addition, TTD analysis showed that the 8 diseases were significantly association with more than 3 formula groups, such as acne, Behcet'S disease, benign prostate hyperplasia, intrahepatic cholestasis and brain injury (Fig. 3B). However, the five formulae contained their specific (MSXG, SGMH, XCH, WLS and Others) GO, KEGG and TTD terms. For example, neurological system process and beta-Alanine metabolism terms were specific for MXSG; membrane organization and parasitic infections of the eye terms for SGMH; circadian rhythm and hypertension terms for XCH; nucleic acid binding transcription factor activity, ovarian steroidogenesis and chronic inflammatory diseases terms for WLS; fat digestion and absorption terms for Others.\nFig. 2 Bubble plot of the GO analysis of the five formulae’ targets.\nFig. 3 Bubble plot of the KEGG/TTD analysis of the five formulae’ targets. A: KEGG, B: TTD.\nIn the prediction of miRNAs in QFPD targets, MIR-183 and MIR-130A/B/301 were the highest linking terms to bind the five formulae targets and formulae Others was the highest group to bind miRNAs (Fig. 4 A). In addition, kinase prediction revealed CDK7 were significantly enriched in formulae MSXG, SGMH, XCH, WLS and Others (Fig. 4B). Finally, TF analysis showed that LXR was the highest linking TF to bind the four formulae targets and formulae WLS was the highest group to bind TFs (Fig. 4C).\nFig. 4 The miRNA, kinase and TF analysis of the five formulae’ targets by WebGestalt. Chord plot showing the five formulae’ targets present in the represented enriched miRNA, kinase and TF terms. Outer ring shows miRNA/kinase/TF term and log2 enrichment ratio (left) or five formulae grouping (right). Chords connect miRNA/kinase/TF term with formulae groups. 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