PMC:7196351 / 10040-10664 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7196351","sourcedb":"PMC","sourceid":"7196351","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7196351","text":"Numbers and activation of splenic leukocytes were analysed by flowcytometry (FACSAria Fusion, BD Biosciences) as described (12). Splenic cells (106) were incubated in 100 µl FACS buffer (phosphate buffered saline (PBS) plus 0.1% FCS) containing fluorochrome-conjugated antibodies at a concentration of 10 µg/ml. The cells were stained with fluorochrome-coupled antibodies (eBioscience, USA) to CD45, CD3, CD4, CD8, CD19, CD11b, CD11c, F4/80, I-A/I-E and CD69. After incubating with the Abs for 60 min at 4 oC, cells were washed twice and resuspended in FACS buffer. A total of 2 x 104 cells were analysed with flowcytometry.","tracks":[{"project":"2_test","denotations":[{"id":"32373300-25472594-54158469","span":{"begin":124,"end":126},"obj":"25472594"},{"id":"T61906","span":{"begin":124,"end":126},"obj":"25472594"}],"attributes":[{"subj":"32373300-25472594-54158469","pred":"source","obj":"2_test"},{"subj":"T61906","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#c293ec","default":true}]}]}}