PMC:7172841 / 31778-32085 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T213","span":{"begin":31,"end":36},"obj":"Body_part"},{"id":"T214","span":{"begin":285,"end":300},"obj":"Body_part"},{"id":"T215","span":{"begin":295,"end":300},"obj":"Body_part"}],"attributes":[{"id":"A213","pred":"fma_id","subj":"T213","obj":"http://purl.org/sig/ont/fma/fma9670"},{"id":"A214","pred":"fma_id","subj":"T214","obj":"http://purl.org/sig/ont/fma/fma62845"},{"id":"A215","pred":"fma_id","subj":"T215","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"In separate experiments, whole blood was treated with 10 μg/mL brefeldin A (Invitrogen, Carlsbad, CA, USA) for 60 min at 37°C in 5% CO2 followed by incubation in the dark for 15 min at room temperature with anti-CD4 FITC, anti-CD14 FITC (Beckman Coulter) and anti-CD45 PC5 followed by red blood cells lysis."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T29","span":{"begin":31,"end":36},"obj":"Body_part"},{"id":"T30","span":{"begin":289,"end":294},"obj":"Body_part"}],"attributes":[{"id":"A29","pred":"uberon_id","subj":"T29","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A30","pred":"uberon_id","subj":"T30","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"In separate experiments, whole blood was treated with 10 μg/mL brefeldin A (Invitrogen, Carlsbad, CA, USA) for 60 min at 37°C in 5% CO2 followed by incubation in the dark for 15 min at room temperature with anti-CD4 FITC, anti-CD14 FITC (Beckman Coulter) and anti-CD45 PC5 followed by red blood cells lysis."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T284","span":{"begin":25,"end":36},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T285","span":{"begin":25,"end":36},"obj":"http://www.ebi.ac.uk/efo/EFO_0000296"},{"id":"T286","span":{"begin":73,"end":74},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T287","span":{"begin":212,"end":215},"obj":"http://purl.obolibrary.org/obo/PR_000001004"},{"id":"T288","span":{"begin":285,"end":300},"obj":"http://purl.obolibrary.org/obo/CL_0000232"}],"text":"In separate experiments, whole blood was treated with 10 μg/mL brefeldin A (Invitrogen, Carlsbad, CA, USA) for 60 min at 37°C in 5% CO2 followed by incubation in the dark for 15 min at room temperature with anti-CD4 FITC, anti-CD14 FITC (Beckman Coulter) and anti-CD45 PC5 followed by red blood cells lysis."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T245","span":{"begin":63,"end":74},"obj":"Chemical"},{"id":"T246","span":{"begin":132,"end":135},"obj":"Chemical"},{"id":"T247","span":{"begin":216,"end":220},"obj":"Chemical"},{"id":"T248","span":{"begin":232,"end":236},"obj":"Chemical"}],"attributes":[{"id":"A245","pred":"chebi_id","subj":"T245","obj":"http://purl.obolibrary.org/obo/CHEBI_48080"},{"id":"A246","pred":"chebi_id","subj":"T246","obj":"http://purl.obolibrary.org/obo/CHEBI_16526"},{"id":"A247","pred":"chebi_id","subj":"T247","obj":"http://purl.obolibrary.org/obo/CHEBI_37926"},{"id":"A248","pred":"chebi_id","subj":"T248","obj":"http://purl.obolibrary.org/obo/CHEBI_37926"}],"text":"In separate experiments, whole blood was treated with 10 μg/mL brefeldin A (Invitrogen, Carlsbad, CA, USA) for 60 min at 37°C in 5% CO2 followed by incubation in the dark for 15 min at room temperature with anti-CD4 FITC, anti-CD14 FITC (Beckman Coulter) and anti-CD45 PC5 followed by red blood cells lysis."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T34","span":{"begin":301,"end":306},"obj":"http://purl.obolibrary.org/obo/GO_0019835"}],"text":"In separate experiments, whole blood was treated with 10 μg/mL brefeldin A (Invitrogen, Carlsbad, CA, USA) for 60 min at 37°C in 5% CO2 followed by incubation in the dark for 15 min at room temperature with anti-CD4 FITC, anti-CD14 FITC (Beckman Coulter) and anti-CD45 PC5 followed by red blood cells lysis."}

{"project":"LitCovid-sentences","denotations":[{"id":"T252","span":{"begin":0,"end":307},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"In separate experiments, whole blood was treated with 10 μg/mL brefeldin A (Invitrogen, Carlsbad, CA, USA) for 60 min at 37°C in 5% CO2 followed by incubation in the dark for 15 min at room temperature with anti-CD4 FITC, anti-CD14 FITC (Beckman Coulter) and anti-CD45 PC5 followed by red blood cells lysis."}

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T902","span":{"begin":31,"end":36},"obj":"UBERON:0000178"},{"id":"T903","span":{"begin":63,"end":74},"obj":"CHEBI:48080;CHEBI:48080"},{"id":"T904","span":{"begin":132,"end":135},"obj":"CHEBI:16526;CHEBI:16526"},{"id":"T905","span":{"begin":212,"end":215},"obj":"PR:000001004"},{"id":"T906","span":{"begin":216,"end":220},"obj":"CHEBI:37926;CHEBI:37926"},{"id":"T907","span":{"begin":227,"end":231},"obj":"PR:000001889"},{"id":"T908","span":{"begin":232,"end":236},"obj":"CHEBI:37926;CHEBI:37926"},{"id":"T909","span":{"begin":264,"end":268},"obj":"PR:000001006"},{"id":"T910","span":{"begin":269,"end":272},"obj":"PR:000012437"},{"id":"T911","span":{"begin":285,"end":288},"obj":"CL:0000232"},{"id":"T912","span":{"begin":289,"end":294},"obj":"CL:0000232;UBERON:0000178"},{"id":"T913","span":{"begin":295,"end":300},"obj":"CL:0000232;GO:0019835"},{"id":"T914","span":{"begin":301,"end":306},"obj":"GO:0019835"},{"id":"T59635","span":{"begin":31,"end":36},"obj":"UBERON:0000178"},{"id":"T72628","span":{"begin":63,"end":74},"obj":"CHEBI:48080;CHEBI:48080"},{"id":"T55076","span":{"begin":132,"end":135},"obj":"CHEBI:16526;CHEBI:16526"},{"id":"T37643","span":{"begin":212,"end":215},"obj":"PR:000001004"},{"id":"T96160","span":{"begin":216,"end":220},"obj":"CHEBI:37926;CHEBI:37926"},{"id":"T30151","span":{"begin":227,"end":231},"obj":"PR:000001889"},{"id":"T57488","span":{"begin":232,"end":236},"obj":"CHEBI:37926;CHEBI:37926"},{"id":"T70801","span":{"begin":264,"end":268},"obj":"PR:000001006"},{"id":"T18750","span":{"begin":269,"end":272},"obj":"PR:000012437"},{"id":"T27300","span":{"begin":285,"end":288},"obj":"CL:0000232"},{"id":"T32412","span":{"begin":289,"end":294},"obj":"CL:0000232;UBERON:0000178"},{"id":"T3825","span":{"begin":295,"end":300},"obj":"CL:0000232;GO:0019835"},{"id":"T62107","span":{"begin":301,"end":306},"obj":"GO:0019835"}],"text":"In separate experiments, whole blood was treated with 10 μg/mL brefeldin A (Invitrogen, Carlsbad, CA, USA) for 60 min at 37°C in 5% CO2 followed by incubation in the dark for 15 min at room temperature with anti-CD4 FITC, anti-CD14 FITC (Beckman Coulter) and anti-CD45 PC5 followed by red blood cells lysis."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1142","span":{"begin":212,"end":215},"obj":"Gene"},{"id":"1143","span":{"begin":227,"end":231},"obj":"Gene"},{"id":"1144","span":{"begin":264,"end":268},"obj":"Gene"},{"id":"1145","span":{"begin":63,"end":74},"obj":"Chemical"},{"id":"1146","span":{"begin":132,"end":135},"obj":"Chemical"},{"id":"1147","span":{"begin":216,"end":220},"obj":"Chemical"},{"id":"1148","span":{"begin":232,"end":236},"obj":"Chemical"}],"attributes":[{"id":"A1142","pred":"tao:has_database_id","subj":"1142","obj":"Gene:920"},{"id":"A1143","pred":"tao:has_database_id","subj":"1143","obj":"Gene:929"},{"id":"A1144","pred":"tao:has_database_id","subj":"1144","obj":"Gene:5788"},{"id":"A1145","pred":"tao:has_database_id","subj":"1145","obj":"MESH:D020126"},{"id":"A1146","pred":"tao:has_database_id","subj":"1146","obj":"MESH:D002245"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"In separate experiments, whole blood was treated with 10 μg/mL brefeldin A (Invitrogen, Carlsbad, CA, USA) for 60 min at 37°C in 5% CO2 followed by incubation in the dark for 15 min at room temperature with anti-CD4 FITC, anti-CD14 FITC (Beckman Coulter) and anti-CD45 PC5 followed by red blood cells lysis."}