PMC:7172841 / 26476-28636 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T182","span":{"begin":58,"end":68},"obj":"Body_part"},{"id":"T183","span":{"begin":206,"end":209},"obj":"Body_part"},{"id":"T184","span":{"begin":466,"end":474},"obj":"Body_part"},{"id":"T185","span":{"begin":567,"end":575},"obj":"Body_part"},{"id":"T186","span":{"begin":652,"end":660},"obj":"Body_part"},{"id":"T187","span":{"begin":757,"end":765},"obj":"Body_part"},{"id":"T188","span":{"begin":838,"end":841},"obj":"Body_part"},{"id":"T189","span":{"begin":850,"end":858},"obj":"Body_part"},{"id":"T190","span":{"begin":1032,"end":1040},"obj":"Body_part"},{"id":"T191","span":{"begin":1273,"end":1282},"obj":"Body_part"},{"id":"T192","span":{"begin":1323,"end":1333},"obj":"Body_part"},{"id":"T193","span":{"begin":1487,"end":1492},"obj":"Body_part"},{"id":"T194","span":{"begin":1638,"end":1657},"obj":"Body_part"},{"id":"T195","span":{"begin":1740,"end":1744},"obj":"Body_part"}],"attributes":[{"id":"A182","pred":"fma_id","subj":"T182","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A183","pred":"fma_id","subj":"T183","obj":"http://purl.org/sig/ont/fma/fma84795"},{"id":"A184","pred":"fma_id","subj":"T184","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A185","pred":"fma_id","subj":"T185","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A186","pred":"fma_id","subj":"T186","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A187","pred":"fma_id","subj":"T187","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A188","pred":"fma_id","subj":"T188","obj":"http://purl.org/sig/ont/fma/fma84795"},{"id":"A189","pred":"fma_id","subj":"T189","obj":"http://purl.org/sig/ont/fma/fma62864"},{"id":"A190","pred":"fma_id","subj":"T190","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A191","pred":"fma_id","subj":"T191","obj":"http://purl.org/sig/ont/fma/fma82752"},{"id":"A192","pred":"fma_id","subj":"T192","obj":"http://purl.org/sig/ont/fma/fma62863"},{"id":"A193","pred":"fma_id","subj":"T193","obj":"http://purl.org/sig/ont/fma/fma63083"},{"id":"A194","pred":"fma_id","subj":"T194","obj":"http://purl.org/sig/ont/fma/fma82785"},{"id":"A195","pred":"fma_id","subj":"T195","obj":"http://purl.org/sig/ont/fma/fma86583"}],"text":"Key Resources Table\nREAGENT or RESOURCE SOURCE IDENTIFIER\nAntibodies\nCD14-FITC (RMO52 clone) Beckman Coulter Cat#IM0645U; RRID: AB_130992\nCD45-PC5 (J33 clone) Beckman Coulter Cat#IM2653U; RRID: AB_10641226\nHLA-DR-RD1 (9-49 clone) Beckman Coulter Cat#6604366; RRID: AB_2832962\nIgG1-PE isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07796; RRID: AB_2832963\nIgG1-FITC isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07795; RRID: AB_2832964\nCD3-FITC/CD4-PE Antibody Cocktail (UCHT1/13b8.2 clones) Beckman Coulter Cat#A07733; RRID: AB_2832965\nCD3-FITC/CD8-PE Antibody Cocktail Beckman Coulter Cat#A07734; RRID: AB_2832966\nCD3-FITC/CD(16+56)-PE Antibody Cocktail (UCHT1/3G8/N901 (NKH-1)) Beckman Coulter Cat#A07735; RRID: AB_1575965\nCD19-FITC/CD5-PE Antibody Cocktail Beckman Coulter Cat#IM1346U; RRID: AB_2832967\nQuantibrite Anti-HLA-DR/Anti-Monocyte (L243/MϕP9) BD Biosciences Cat#340827; RRID: AB_400137\nMouse Anti-Human IL-6-PE (AS12 clone) BD Biosciences Cat#340527; RRID: AB_400442\nChemicals, Peptides, and Recombinant Proteins\nVersaLyse Lysing Solution Beckman Coulter A09777\nFixative Solution Beckman Coulter A07800\neBioscience™ Brefeldin A Solution (1000x) Invitrogen 00-4506-51\nIntraPrep Permeabilization Reagent Beckman Coulter A07802\nRPMI 1640 W/ Stable Glutamine W/ 25 MM HEPES Biowest L0496\nLymphosep, Lymphocyte Separation Media Biowest L0560\nPBS Dulbecco’s Phosphate Buffered Saline w/o Magnesium, w/o Calcium Biowest L0615\nFBS Superior; standardized Fetal Bovine Serum, EU-approved Biochrom S0615\nGentamycin Sulfate BioChemica PanReac AppliChem A1492\nPenicillin G Potassium Salt BioChemica PanReac AppliChem A1837\nLipopolysaccharides from Escherichia coli O55:B5 Sigma-Aldrich L2880\nCritical Commercial Assays\nHuman IL-1β uncoated ELISA Invitrogen 88-7261\nHuman TNF-α uncoated ELISA Invitrogen 88-7346\nHuman IL-6 ELISA Invitrogen 88-7066\nHuman IL-17A (homodimer) uncoated ELISA Invitrogen 88-7176\nHuman Ferritin ELISA ORGENTEC Diagnostika GmbH ORG 5FE\nHuman IFN-γ ELISA Diaclone 950.000.192\nSoftware and Algorithms\nGraphPad Prism Graphpad Software https://www.graphpad.com\nSPSS IBM https://www.ibm.com/analytics/spss-statistics-software"}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T21","span":{"begin":1487,"end":1492},"obj":"Body_part"}],"attributes":[{"id":"A21","pred":"uberon_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"Key Resources Table\nREAGENT or RESOURCE SOURCE IDENTIFIER\nAntibodies\nCD14-FITC (RMO52 clone) Beckman Coulter Cat#IM0645U; RRID: AB_130992\nCD45-PC5 (J33 clone) Beckman Coulter Cat#IM2653U; RRID: AB_10641226\nHLA-DR-RD1 (9-49 clone) Beckman Coulter Cat#6604366; RRID: AB_2832962\nIgG1-PE isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07796; RRID: AB_2832963\nIgG1-FITC isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07795; RRID: AB_2832964\nCD3-FITC/CD4-PE Antibody Cocktail (UCHT1/13b8.2 clones) Beckman Coulter Cat#A07733; RRID: AB_2832965\nCD3-FITC/CD8-PE Antibody Cocktail Beckman Coulter Cat#A07734; RRID: AB_2832966\nCD3-FITC/CD(16+56)-PE Antibody Cocktail (UCHT1/3G8/N901 (NKH-1)) Beckman Coulter Cat#A07735; RRID: AB_1575965\nCD19-FITC/CD5-PE Antibody Cocktail Beckman Coulter Cat#IM1346U; RRID: AB_2832967\nQuantibrite Anti-HLA-DR/Anti-Monocyte (L243/MϕP9) BD Biosciences Cat#340827; RRID: AB_400137\nMouse Anti-Human IL-6-PE (AS12 clone) BD Biosciences Cat#340527; RRID: AB_400442\nChemicals, Peptides, and Recombinant Proteins\nVersaLyse Lysing Solution Beckman Coulter A09777\nFixative Solution Beckman Coulter A07800\neBioscience™ Brefeldin A Solution (1000x) Invitrogen 00-4506-51\nIntraPrep Permeabilization Reagent Beckman Coulter A07802\nRPMI 1640 W/ Stable Glutamine W/ 25 MM HEPES Biowest L0496\nLymphosep, Lymphocyte Separation Media Biowest L0560\nPBS Dulbecco’s Phosphate Buffered Saline w/o Magnesium, w/o Calcium Biowest L0615\nFBS Superior; standardized Fetal Bovine Serum, EU-approved Biochrom S0615\nGentamycin Sulfate BioChemica PanReac AppliChem A1492\nPenicillin G Potassium Salt BioChemica PanReac AppliChem A1837\nLipopolysaccharides from Escherichia coli O55:B5 Sigma-Aldrich L2880\nCritical Commercial Assays\nHuman IL-1β uncoated ELISA Invitrogen 88-7261\nHuman TNF-α uncoated ELISA Invitrogen 88-7346\nHuman IL-6 ELISA Invitrogen 88-7066\nHuman IL-17A (homodimer) uncoated ELISA Invitrogen 88-7176\nHuman Ferritin ELISA ORGENTEC Diagnostika GmbH ORG 5FE\nHuman IFN-γ ELISA Diaclone 950.000.192\nSoftware and Algorithms\nGraphPad Prism Graphpad Software https://www.graphpad.com\nSPSS IBM https://www.ibm.com/analytics/spss-statistics-software"}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T229","span":{"begin":871,"end":873},"obj":"Disease"},{"id":"T230","span":{"begin":952,"end":954},"obj":"Disease"}],"attributes":[{"id":"A229","pred":"mondo_id","subj":"T229","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"},{"id":"A230","pred":"mondo_id","subj":"T230","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"}],"text":"Key Resources Table\nREAGENT or RESOURCE SOURCE IDENTIFIER\nAntibodies\nCD14-FITC (RMO52 clone) Beckman Coulter Cat#IM0645U; RRID: AB_130992\nCD45-PC5 (J33 clone) Beckman Coulter Cat#IM2653U; RRID: AB_10641226\nHLA-DR-RD1 (9-49 clone) Beckman Coulter Cat#6604366; RRID: AB_2832962\nIgG1-PE isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07796; RRID: AB_2832963\nIgG1-FITC isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07795; RRID: AB_2832964\nCD3-FITC/CD4-PE Antibody Cocktail (UCHT1/13b8.2 clones) Beckman Coulter Cat#A07733; RRID: AB_2832965\nCD3-FITC/CD8-PE Antibody Cocktail Beckman Coulter Cat#A07734; RRID: AB_2832966\nCD3-FITC/CD(16+56)-PE Antibody Cocktail (UCHT1/3G8/N901 (NKH-1)) Beckman Coulter Cat#A07735; RRID: AB_1575965\nCD19-FITC/CD5-PE Antibody Cocktail Beckman Coulter Cat#IM1346U; RRID: AB_2832967\nQuantibrite Anti-HLA-DR/Anti-Monocyte (L243/MϕP9) BD Biosciences Cat#340827; RRID: AB_400137\nMouse Anti-Human IL-6-PE (AS12 clone) BD Biosciences Cat#340527; RRID: AB_400442\nChemicals, Peptides, and Recombinant Proteins\nVersaLyse Lysing Solution Beckman Coulter A09777\nFixative Solution Beckman Coulter A07800\neBioscience™ Brefeldin A Solution (1000x) Invitrogen 00-4506-51\nIntraPrep Permeabilization Reagent Beckman Coulter A07802\nRPMI 1640 W/ Stable Glutamine W/ 25 MM HEPES Biowest L0496\nLymphosep, Lymphocyte Separation Media Biowest L0560\nPBS Dulbecco’s Phosphate Buffered Saline w/o Magnesium, w/o Calcium Biowest L0615\nFBS Superior; standardized Fetal Bovine Serum, EU-approved Biochrom S0615\nGentamycin Sulfate BioChemica PanReac AppliChem A1492\nPenicillin G Potassium Salt BioChemica PanReac AppliChem A1837\nLipopolysaccharides from Escherichia coli O55:B5 Sigma-Aldrich L2880\nCritical Commercial Assays\nHuman IL-1β uncoated ELISA Invitrogen 88-7261\nHuman TNF-α uncoated ELISA Invitrogen 88-7346\nHuman IL-6 ELISA Invitrogen 88-7066\nHuman IL-17A (homodimer) uncoated ELISA Invitrogen 88-7176\nHuman Ferritin ELISA ORGENTEC Diagnostika GmbH ORG 5FE\nHuman IFN-γ ELISA Diaclone 950.000.192\nSoftware and Algorithms\nGraphPad Prism Graphpad Software https://www.graphpad.com\nSPSS IBM https://www.ibm.com/analytics/spss-statistics-software"}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T237","span":{"begin":450,"end":453},"obj":"http://purl.obolibrary.org/obo/CLO_0052882"},{"id":"T238","span":{"begin":450,"end":453},"obj":"http://purl.obolibrary.org/obo/CLO_0053434"},{"id":"T239","span":{"begin":459,"end":462},"obj":"http://purl.obolibrary.org/obo/PR_000001004"},{"id":"T240","span":{"begin":551,"end":554},"obj":"http://purl.obolibrary.org/obo/CLO_0052882"},{"id":"T241","span":{"begin":551,"end":554},"obj":"http://purl.obolibrary.org/obo/CLO_0053434"},{"id":"T242","span":{"begin":560,"end":563},"obj":"http://purl.obolibrary.org/obo/CLO_0053438"},{"id":"T243","span":{"begin":630,"end":633},"obj":"http://purl.obolibrary.org/obo/CLO_0052882"},{"id":"T244","span":{"begin":630,"end":633},"obj":"http://purl.obolibrary.org/obo/CLO_0053434"},{"id":"T245","span":{"begin":740,"end":744},"obj":"http://purl.obolibrary.org/obo/CLO_0053432"},{"id":"T246","span":{"begin":750,"end":753},"obj":"http://purl.obolibrary.org/obo/PR_000001839"},{"id":"T247","span":{"begin":850,"end":858},"obj":"http://purl.obolibrary.org/obo/CL_0000576"},{"id":"T248","span":{"begin":860,"end":864},"obj":"http://purl.obolibrary.org/obo/CLO_0007180"},{"id":"T249","span":{"begin":867,"end":869},"obj":"http://purl.obolibrary.org/obo/CLO_0008343"},{"id":"T250","span":{"begin":914,"end":919},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"},{"id":"T251","span":{"begin":925,"end":930},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T252","span":{"begin":1006,"end":1014},"obj":"http://purl.obolibrary.org/obo/PR_000018263"},{"id":"T253","span":{"begin":1154,"end":1155},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T254","span":{"begin":1684,"end":1686},"obj":"http://purl.obolibrary.org/obo/CLO_0001818"},{"id":"T255","span":{"begin":1734,"end":1739},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T256","span":{"begin":1780,"end":1785},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T257","span":{"begin":1826,"end":1831},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T258","span":{"begin":1862,"end":1867},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T259","span":{"begin":1921,"end":1926},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T260","span":{"begin":1976,"end":1981},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"}],"text":"Key Resources Table\nREAGENT or RESOURCE SOURCE IDENTIFIER\nAntibodies\nCD14-FITC (RMO52 clone) Beckman Coulter Cat#IM0645U; RRID: AB_130992\nCD45-PC5 (J33 clone) Beckman Coulter Cat#IM2653U; RRID: AB_10641226\nHLA-DR-RD1 (9-49 clone) Beckman Coulter Cat#6604366; RRID: AB_2832962\nIgG1-PE isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07796; RRID: AB_2832963\nIgG1-FITC isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07795; RRID: AB_2832964\nCD3-FITC/CD4-PE Antibody Cocktail (UCHT1/13b8.2 clones) Beckman Coulter Cat#A07733; RRID: AB_2832965\nCD3-FITC/CD8-PE Antibody Cocktail Beckman Coulter Cat#A07734; RRID: AB_2832966\nCD3-FITC/CD(16+56)-PE Antibody Cocktail (UCHT1/3G8/N901 (NKH-1)) Beckman Coulter Cat#A07735; RRID: AB_1575965\nCD19-FITC/CD5-PE Antibody Cocktail Beckman Coulter Cat#IM1346U; RRID: AB_2832967\nQuantibrite Anti-HLA-DR/Anti-Monocyte (L243/MϕP9) BD Biosciences Cat#340827; RRID: AB_400137\nMouse Anti-Human IL-6-PE (AS12 clone) BD Biosciences Cat#340527; RRID: AB_400442\nChemicals, Peptides, and Recombinant Proteins\nVersaLyse Lysing Solution Beckman Coulter A09777\nFixative Solution Beckman Coulter A07800\neBioscience™ Brefeldin A Solution (1000x) Invitrogen 00-4506-51\nIntraPrep Permeabilization Reagent Beckman Coulter A07802\nRPMI 1640 W/ Stable Glutamine W/ 25 MM HEPES Biowest L0496\nLymphosep, Lymphocyte Separation Media Biowest L0560\nPBS Dulbecco’s Phosphate Buffered Saline w/o Magnesium, w/o Calcium Biowest L0615\nFBS Superior; standardized Fetal Bovine Serum, EU-approved Biochrom S0615\nGentamycin Sulfate BioChemica PanReac AppliChem A1492\nPenicillin G Potassium Salt BioChemica PanReac AppliChem A1837\nLipopolysaccharides from Escherichia coli O55:B5 Sigma-Aldrich L2880\nCritical Commercial Assays\nHuman IL-1β uncoated ELISA Invitrogen 88-7261\nHuman TNF-α uncoated ELISA Invitrogen 88-7346\nHuman IL-6 ELISA Invitrogen 88-7066\nHuman IL-17A (homodimer) uncoated ELISA Invitrogen 88-7176\nHuman Ferritin ELISA ORGENTEC Diagnostika GmbH ORG 5FE\nHuman IFN-γ ELISA Diaclone 950.000.192\nSoftware and Algorithms\nGraphPad Prism Graphpad Software https://www.graphpad.com\nSPSS IBM https://www.ibm.com/analytics/spss-statistics-software"}

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RRID: AB_130992\nCD45-PC5 (J33 clone) Beckman Coulter Cat#IM2653U; RRID: AB_10641226\nHLA-DR-RD1 (9-49 clone) Beckman Coulter Cat#6604366; RRID: AB_2832962\nIgG1-PE isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07796; RRID: AB_2832963\nIgG1-FITC isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07795; RRID: AB_2832964\nCD3-FITC/CD4-PE Antibody Cocktail (UCHT1/13b8.2 clones) Beckman Coulter Cat#A07733; RRID: AB_2832965\nCD3-FITC/CD8-PE Antibody Cocktail Beckman Coulter Cat#A07734; RRID: AB_2832966\nCD3-FITC/CD(16+56)-PE Antibody Cocktail (UCHT1/3G8/N901 (NKH-1)) Beckman Coulter Cat#A07735; RRID: AB_1575965\nCD19-FITC/CD5-PE Antibody Cocktail Beckman Coulter Cat#IM1346U; RRID: AB_2832967\nQuantibrite Anti-HLA-DR/Anti-Monocyte (L243/MϕP9) BD Biosciences Cat#340827; RRID: AB_400137\nMouse Anti-Human IL-6-PE (AS12 clone) BD Biosciences Cat#340527; RRID: AB_400442\nChemicals, Peptides, and Recombinant Proteins\nVersaLyse Lysing Solution Beckman Coulter A09777\nFixative Solution Beckman Coulter A07800\neBioscience™ Brefeldin A Solution (1000x) Invitrogen 00-4506-51\nIntraPrep Permeabilization Reagent Beckman Coulter A07802\nRPMI 1640 W/ Stable Glutamine W/ 25 MM HEPES Biowest L0496\nLymphosep, Lymphocyte Separation Media Biowest L0560\nPBS Dulbecco’s Phosphate Buffered Saline w/o Magnesium, w/o Calcium Biowest L0615\nFBS Superior; standardized Fetal Bovine Serum, EU-approved Biochrom S0615\nGentamycin Sulfate BioChemica PanReac AppliChem A1492\nPenicillin G Potassium Salt BioChemica PanReac AppliChem A1837\nLipopolysaccharides from Escherichia coli O55:B5 Sigma-Aldrich L2880\nCritical Commercial Assays\nHuman IL-1β uncoated ELISA Invitrogen 88-7261\nHuman TNF-α uncoated ELISA Invitrogen 88-7346\nHuman IL-6 ELISA Invitrogen 88-7066\nHuman IL-17A (homodimer) uncoated ELISA Invitrogen 88-7176\nHuman Ferritin ELISA ORGENTEC Diagnostika GmbH ORG 5FE\nHuman IFN-γ ELISA Diaclone 950.000.192\nSoftware and Algorithms\nGraphPad Prism Graphpad Software https://www.graphpad.com\nSPSS IBM https://www.ibm.com/analytics/spss-statistics-software"}

{"project":"LitCovid-PubTator","denotations":[{"id":"1043","span":{"begin":69,"end":73},"obj":"Gene"},{"id":"1044","span":{"begin":459,"end":462},"obj":"Gene"},{"id":"1045","span":{"begin":560,"end":563},"obj":"Gene"},{"id":"1046","span":{"begin":740,"end":744},"obj":"Gene"},{"id":"1047","span":{"begin":750,"end":753},"obj":"Gene"},{"id":"1048","span":{"begin":1740,"end":1745},"obj":"Gene"},{"id":"1049","span":{"begin":1786,"end":1791},"obj":"Gene"},{"id":"1050","span":{"begin":1832,"end":1836},"obj":"Gene"},{"id":"1051","span":{"begin":1868,"end":1874},"obj":"Gene"},{"id":"1052","span":{"begin":1876,"end":1885},"obj":"Gene"},{"id":"1053","span":{"begin":1982,"end":1987},"obj":"Gene"},{"id":"1054","span":{"begin":138,"end":142},"obj":"Gene"},{"id":"1055","span":{"begin":1663,"end":1683},"obj":"Species"},{"id":"1056","span":{"begin":1734,"end":1739},"obj":"Species"},{"id":"1057","span":{"begin":1780,"end":1785},"obj":"Species"},{"id":"1058","span":{"begin":1826,"end":1831},"obj":"Species"},{"id":"1059","span":{"begin":1862,"end":1867},"obj":"Species"},{"id":"1060","span":{"begin":1921,"end":1926},"obj":"Species"},{"id":"1061","span":{"begin":1976,"end":1981},"obj":"Species"},{"id":"1062","span":{"begin":925,"end":930},"obj":"Species"},{"id":"1063","span":{"begin":73,"end":78},"obj":"Chemical"},{"id":"1064","span":{"begin":687,"end":690},"obj":"Disease"},{"id":"1065","span":{"begin":2028,"end":2053},"obj":"Disease"}],"attributes":[{"id":"A1043","pred":"tao:has_database_id","subj":"1043","obj":"Gene:929"},{"id":"A1044","pred":"tao:has_database_id","subj":"1044","obj":"Gene:920"},{"id":"A1045","pred":"tao:has_database_id","subj":"1045","obj":"Gene:925"},{"id":"A1046","pred":"tao:has_database_id","subj":"1046","obj":"Gene:930"},{"id":"A1047","pred":"tao:has_database_id","subj":"1047","obj":"Gene:921"},{"id":"A1048","pred":"tao:has_database_id","subj":"1048","obj":"Gene:3552"},{"id":"A1049","pred":"tao:has_database_id","subj":"1049","obj":"Gene:7124"},{"id":"A1050","pred":"tao:has_database_id","subj":"1050","obj":"Gene:3569"},{"id":"A1051","pred":"tao:has_database_id","subj":"1051","obj":"Gene:3605"},{"id":"A1052","pred":"tao:has_database_id","subj":"1052","obj":"Gene:6647"},{"id":"A1053","pred":"tao:has_database_id","subj":"1053","obj":"Gene:3458"},{"id":"A1054","pred":"tao:has_database_id","subj":"1054","obj":"Gene:5788"},{"id":"A1055","pred":"tao:has_database_id","subj":"1055","obj":"Tax:2170726"},{"id":"A1056","pred":"tao:has_database_id","subj":"1056","obj":"Tax:9606"},{"id":"A1057","pred":"tao:has_database_id","subj":"1057","obj":"Tax:9606"},{"id":"A1058","pred":"tao:has_database_id","subj":"1058","obj":"Tax:9606"},{"id":"A1059","pred":"tao:has_database_id","subj":"1059","obj":"Tax:9606"},{"id":"A1060","pred":"tao:has_database_id","subj":"1060","obj":"Tax:9606"},{"id":"A1061","pred":"tao:has_database_id","subj":"1061","obj":"Tax:9606"},{"id":"A1062","pred":"tao:has_database_id","subj":"1062","obj":"Tax:9606"},{"id":"A1064","pred":"tao:has_database_id","subj":"1064","obj":"MESH:D020158"},{"id":"A1065","pred":"tao:has_database_id","subj":"1065","obj":"MESH:D007859"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Key Resources Table\nREAGENT or RESOURCE SOURCE IDENTIFIER\nAntibodies\nCD14-FITC (RMO52 clone) Beckman Coulter Cat#IM0645U; RRID: AB_130992\nCD45-PC5 (J33 clone) Beckman Coulter Cat#IM2653U; RRID: AB_10641226\nHLA-DR-RD1 (9-49 clone) Beckman Coulter Cat#6604366; RRID: AB_2832962\nIgG1-PE isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07796; RRID: AB_2832963\nIgG1-FITC isotype control (679.1Mc7 clone) Beckman Coulter Cat#A07795; RRID: AB_2832964\nCD3-FITC/CD4-PE Antibody Cocktail (UCHT1/13b8.2 clones) Beckman Coulter Cat#A07733; RRID: AB_2832965\nCD3-FITC/CD8-PE Antibody Cocktail Beckman Coulter Cat#A07734; RRID: AB_2832966\nCD3-FITC/CD(16+56)-PE Antibody Cocktail (UCHT1/3G8/N901 (NKH-1)) Beckman Coulter Cat#A07735; RRID: AB_1575965\nCD19-FITC/CD5-PE Antibody Cocktail Beckman Coulter Cat#IM1346U; RRID: AB_2832967\nQuantibrite Anti-HLA-DR/Anti-Monocyte (L243/MϕP9) BD Biosciences Cat#340827; RRID: AB_400137\nMouse Anti-Human IL-6-PE (AS12 clone) BD Biosciences Cat#340527; RRID: AB_400442\nChemicals, Peptides, and Recombinant Proteins\nVersaLyse Lysing Solution Beckman Coulter A09777\nFixative Solution Beckman Coulter A07800\neBioscience™ Brefeldin A Solution (1000x) Invitrogen 00-4506-51\nIntraPrep Permeabilization Reagent Beckman Coulter A07802\nRPMI 1640 W/ Stable Glutamine W/ 25 MM HEPES Biowest L0496\nLymphosep, Lymphocyte Separation Media Biowest L0560\nPBS Dulbecco’s Phosphate Buffered Saline w/o Magnesium, w/o Calcium Biowest L0615\nFBS Superior; standardized Fetal Bovine Serum, EU-approved Biochrom S0615\nGentamycin Sulfate BioChemica PanReac AppliChem A1492\nPenicillin G Potassium Salt BioChemica PanReac AppliChem A1837\nLipopolysaccharides from Escherichia coli O55:B5 Sigma-Aldrich L2880\nCritical Commercial Assays\nHuman IL-1β uncoated ELISA Invitrogen 88-7261\nHuman TNF-α uncoated ELISA Invitrogen 88-7346\nHuman IL-6 ELISA Invitrogen 88-7066\nHuman IL-17A (homodimer) uncoated ELISA Invitrogen 88-7176\nHuman Ferritin ELISA ORGENTEC Diagnostika GmbH ORG 5FE\nHuman IFN-γ ELISA Diaclone 950.000.192\nSoftware and Algorithms\nGraphPad Prism Graphpad Software https://www.graphpad.com\nSPSS IBM https://www.ibm.com/analytics/spss-statistics-software"}