PMC:7151644 / 9545-9898 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7151644","sourcedb":"PMC","sourceid":"7151644","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7151644","text":"The QIAquick Gel Extraction kit (Qiagen) was used to purify the PCR products before sequencing. Purified DNA \u003c700 bp in length was sequenced directly, while those larger than 700 bp were first cloned into a pMD18-T vector (TaKaRa), and then transformed into JM109-143 competent cells. For each sample, at least three clones were selected for sequencing.","tracks":[]}