PMC:7151644 / 9067-9544 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7151644","sourcedb":"PMC","sourceid":"7151644","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7151644","text":"Total RNA was reverse transcribed using a one-step RT-PCR kit (TaKaRa). The viral RNA in ticks was detected by nested PCR targeting the conserved regions of the RNA-dependent RNA polymerase (RdRp) gene of both the pestivirus and coltivirus. To recover complete viral genomes, primers were designed based on the assembled pestivirus and coltivirus contigs obtained by meta-transcriptomics (Supplementary Table S3). The genome termini were determined by 5ʹ/3ʹ RACE kits (TaKaRa).","tracks":[]}