PMC:7151644 / 19737-21347 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7151644","sourcedb":"PMC","sourceid":"7151644","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7151644","text":"3.4 Phylogenetic relationships of the newly identified viruses\nTo determine the evolutionary relationships among the newly identified and previously recognized viruses, we estimated ML phylogenetic trees based on aa sequences (Figs 4 and 5).\nFigure 4. ML trees based on aa sequences of the entire coding sequences (polyprotein) and NS3 genes of DYPV and other known pestiviruses. The numbers at nodes indicate bootstrap support values after 1,000 replications. Bootstrap values higher than 70 per cent were considered significant and shown on the trees.\nFigure 5. ML trees based on aa sequences of the RdRp genes and putative RNA methyltransferase (VP2) genes of LSPV and other known coltiviruses. The numbers at nodes indicate bootstrap support values after 1,000 replications. Bootstrap values higher than 70 per cent were considered significant and shown on the trees. In phylogenies based on the aa sequences of the entire coding sequences and the NS3 genes, DYPV formed a highly distinct lineage, confirming that it represents a novel pestivirus. Notably, the DYPV lineage occupied the basal position within the Artiodactylous lineage, with those pestiviruses present in rodents and bats forming more divergent lineages. In the phylogenies based on the aa sequences of the RdRp gene and putative RNA methyltransferase gene (VP2; Fig. 5), LSPV also formed a distinct lineage within the genus Coltivirus. Interestingly, however, the virus was most closely related to Shelly headland identified in ticks (I. holocyclus) sampled from the long-nosed bandicoot (a marsupial) in Australia (Harvey et al. 2018).","divisions":[{"label":"title","span":{"begin":0,"end":62}},{"label":"p","span":{"begin":63,"end":241}},{"label":"figure","span":{"begin":242,"end":554}},{"label":"label","span":{"begin":242,"end":251}},{"label":"caption","span":{"begin":253,"end":554}},{"label":"p","span":{"begin":253,"end":554}},{"label":"figure","span":{"begin":555,"end":873}},{"label":"label","span":{"begin":555,"end":564}},{"label":"caption","span":{"begin":566,"end":873}},{"label":"p","span":{"begin":566,"end":873}}],"tracks":[]}