PMC:7151644 / 14741-16042 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7151644","sourcedb":"PMC","sourceid":"7151644","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7151644","text":"To identify the possible etiologic agents of disease in the four pangolins, eight meta-transcriptomic libraries from blood, liver, spleen, lung, kidney, and fecal samples were constructed, generating a total of 306,908,179 paired-end sequence reads. De novo assembly revealed the high abundance of a pestivirus- and coltivirus-like virus in all the meta-transcriptomic libraries of the pangolin 1-Dongyang and 2-Lishui, representing 6–80 and 1–29 per cent of total viral contigs, respectively (Supplementary Table S2). Notably, despite the presence of other putative viruses (Supplementary Table S2), only the pestivirus- and coltivirus-like virus could be successfully identified by PCR. As those putative viral sequences could not be confirmed by PCR and probably were from contamination; hence, they were not possible etiologic agents of disease in four pangolins. Additional assays revealed that the novel pestivirus was only present in 1-Dongyang, whereas the novel coltivirus was only present in 2-Lishui. No potential bacterial and fungal pathogens were found in the libraries generated from 1-Dongyang and 2-Lishui. Finally, no abundant viral, bacterial and fungal sequences were found in the libraries generated from blood and tissue samples of 3-Ruian and 4-Wucheng (Supplementary Table S2).","tracks":[]}