PMC:7143804 / 60983-61809 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"489","span":{"begin":64,"end":67},"obj":"Chemical"},{"id":"490","span":{"begin":198,"end":203},"obj":"Chemical"},{"id":"491","span":{"begin":622,"end":627},"obj":"Chemical"}],"attributes":[{"id":"A490","pred":"tao:has_database_id","subj":"490","obj":"MESH:D014867"},{"id":"A491","pred":"tao:has_database_id","subj":"491","obj":"MESH:D014867"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Figure 15 Fluorescence signal of DNA-binded EvaGreen dye after MDA reactions. The reactions are performed inside an Eppendorf tube and on-chip. As heating, two different methods are used, namely a water bath (for both the Eppendorf tube and chip) and the on-chip resistive heater (3 chips). The fluorescence intensity cannot be used as a value to quantify the amount of DNA [44]. The two blue lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside an Eppendorf tube. The two orange lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside a water bath heated chip. The three continuous orange lines with asterisks, squares, and diamonds represent the signals obtained after MDA reactions performed inside chips while using the integrated heater."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T148","span":{"begin":34,"end":37},"obj":"Body_part"},{"id":"T149","span":{"begin":371,"end":374},"obj":"Body_part"}],"attributes":[{"id":"A148","pred":"fma_id","subj":"T148","obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"A149","pred":"fma_id","subj":"T149","obj":"http://purl.org/sig/ont/fma/fma74412"}],"text":"Figure 15 Fluorescence signal of DNA-binded EvaGreen dye after MDA reactions. The reactions are performed inside an Eppendorf tube and on-chip. As heating, two different methods are used, namely a water bath (for both the Eppendorf tube and chip) and the on-chip resistive heater (3 chips). The fluorescence intensity cannot be used as a value to quantify the amount of DNA [44]. The two blue lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside an Eppendorf tube. The two orange lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside a water bath heated chip. The three continuous orange lines with asterisks, squares, and diamonds represent the signals obtained after MDA reactions performed inside chips while using the integrated heater."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T24","span":{"begin":127,"end":131},"obj":"Body_part"},{"id":"T25","span":{"begin":233,"end":237},"obj":"Body_part"},{"id":"T26","span":{"begin":503,"end":507},"obj":"Body_part"}],"attributes":[{"id":"A24","pred":"uberon_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/UBERON_0000025"},{"id":"A25","pred":"uberon_id","subj":"T25","obj":"http://purl.obolibrary.org/obo/UBERON_0000025"},{"id":"A26","pred":"uberon_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/UBERON_0000025"}],"text":"Figure 15 Fluorescence signal of DNA-binded EvaGreen dye after MDA reactions. The reactions are performed inside an Eppendorf tube and on-chip. As heating, two different methods are used, namely a water bath (for both the Eppendorf tube and chip) and the on-chip resistive heater (3 chips). The fluorescence intensity cannot be used as a value to quantify the amount of DNA [44]. The two blue lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside an Eppendorf tube. The two orange lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside a water bath heated chip. The three continuous orange lines with asterisks, squares, and diamonds represent the signals obtained after MDA reactions performed inside chips while using the integrated heater."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T466","span":{"begin":24,"end":30},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T467","span":{"begin":127,"end":131},"obj":"http://purl.obolibrary.org/obo/UBERON_0000025"},{"id":"T468","span":{"begin":196,"end":197},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T469","span":{"begin":233,"end":237},"obj":"http://purl.obolibrary.org/obo/UBERON_0000025"},{"id":"T470","span":{"begin":337,"end":338},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T471","span":{"begin":503,"end":507},"obj":"http://purl.obolibrary.org/obo/UBERON_0000025"},{"id":"T472","span":{"begin":620,"end":621},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T473","span":{"begin":732,"end":739},"obj":"http://purl.obolibrary.org/obo/SO_0000418"}],"text":"Figure 15 Fluorescence signal of DNA-binded EvaGreen dye after MDA reactions. The reactions are performed inside an Eppendorf tube and on-chip. As heating, two different methods are used, namely a water bath (for both the Eppendorf tube and chip) and the on-chip resistive heater (3 chips). The fluorescence intensity cannot be used as a value to quantify the amount of DNA [44]. The two blue lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside an Eppendorf tube. The two orange lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside a water bath heated chip. The three continuous orange lines with asterisks, squares, and diamonds represent the signals obtained after MDA reactions performed inside chips while using the integrated heater."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T44396","span":{"begin":34,"end":37},"obj":"Chemical"},{"id":"T120","span":{"begin":54,"end":57},"obj":"Chemical"},{"id":"T6916","span":{"begin":64,"end":67},"obj":"Chemical"},{"id":"T61261","span":{"begin":198,"end":203},"obj":"Chemical"},{"id":"T123","span":{"begin":371,"end":374},"obj":"Chemical"},{"id":"T50312","span":{"begin":429,"end":432},"obj":"Chemical"},{"id":"T125","span":{"begin":559,"end":562},"obj":"Chemical"},{"id":"T97169","span":{"begin":622,"end":627},"obj":"Chemical"},{"id":"T57260","span":{"begin":755,"end":758},"obj":"Chemical"}],"attributes":[{"id":"A7515","pred":"chebi_id","subj":"T44396","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A68547","pred":"chebi_id","subj":"T120","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"},{"id":"A72221","pred":"chebi_id","subj":"T6916","obj":"http://purl.obolibrary.org/obo/CHEBI_566274"},{"id":"A96115","pred":"chebi_id","subj":"T61261","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"},{"id":"A76969","pred":"chebi_id","subj":"T123","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A29488","pred":"chebi_id","subj":"T50312","obj":"http://purl.obolibrary.org/obo/CHEBI_566274"},{"id":"A11864","pred":"chebi_id","subj":"T125","obj":"http://purl.obolibrary.org/obo/CHEBI_566274"},{"id":"A33610","pred":"chebi_id","subj":"T97169","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"},{"id":"A52319","pred":"chebi_id","subj":"T57260","obj":"http://purl.obolibrary.org/obo/CHEBI_566274"}],"text":"Figure 15 Fluorescence signal of DNA-binded EvaGreen dye after MDA reactions. The reactions are performed inside an Eppendorf tube and on-chip. As heating, two different methods are used, namely a water bath (for both the Eppendorf tube and chip) and the on-chip resistive heater (3 chips). The fluorescence intensity cannot be used as a value to quantify the amount of DNA [44]. The two blue lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside an Eppendorf tube. The two orange lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside a water bath heated chip. The three continuous orange lines with asterisks, squares, and diamonds represent the signals obtained after MDA reactions performed inside chips while using the integrated heater."}

{"project":"LitCovid-sentences","denotations":[{"id":"T546","span":{"begin":0,"end":78},"obj":"Sentence"},{"id":"T547","span":{"begin":79,"end":144},"obj":"Sentence"},{"id":"T548","span":{"begin":145,"end":291},"obj":"Sentence"},{"id":"T549","span":{"begin":292,"end":380},"obj":"Sentence"},{"id":"T550","span":{"begin":381,"end":508},"obj":"Sentence"},{"id":"T551","span":{"begin":509,"end":645},"obj":"Sentence"},{"id":"T552","span":{"begin":646,"end":826},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Figure 15 Fluorescence signal of DNA-binded EvaGreen dye after MDA reactions. The reactions are performed inside an Eppendorf tube and on-chip. As heating, two different methods are used, namely a water bath (for both the Eppendorf tube and chip) and the on-chip resistive heater (3 chips). The fluorescence intensity cannot be used as a value to quantify the amount of DNA [44]. The two blue lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside an Eppendorf tube. The two orange lines with circular points are the MDA (continuous line) and NTC (dashed line) performed inside a water bath heated chip. The three continuous orange lines with asterisks, squares, and diamonds represent the signals obtained after MDA reactions performed inside chips while using the integrated heater."}