PMC:7143804 / 42231-43081 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"296","span":{"begin":119,"end":124},"obj":"Chemical"},{"id":"297","span":{"begin":161,"end":163},"obj":"Chemical"},{"id":"298","span":{"begin":264,"end":269},"obj":"Chemical"},{"id":"299","span":{"begin":471,"end":478},"obj":"Chemical"}],"attributes":[{"id":"A296","pred":"tao:has_database_id","subj":"296","obj":"MESH:D014867"},{"id":"A297","pred":"tao:has_database_id","subj":"297","obj":"MESH:D006046"},{"id":"A298","pred":"tao:has_database_id","subj":"298","obj":"MESH:D014867"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"As can be seen in Figure 15, the spectra of the amplification performed inside the chip, and by applying heat with the water bath as well as with the integrated Au-resistive heater, show the same trend as the amplification performed in the Eppendorf and heated by water bath. There is an order of magnitude difference in the fluorescence signal. However, the fluorescence intensity cannot be used as a value to quantify the amount of DNA. EvaGreen is a bis-intercalating cyanine fluorescence dye consisting of two monomeric DNA-binding dyes which are linked by a flexible spacer. These two DNA-binding dyes bind each in between two base pairs, which make them simple and fast, but also nonuniform and non-specific [44,63]. However, with this dye, a simple yes-or-no answer can be obtained if the amplification took place, as can be seen in Figure 15."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T73","span":{"begin":434,"end":437},"obj":"Body_part"},{"id":"T74","span":{"begin":524,"end":527},"obj":"Body_part"},{"id":"T75","span":{"begin":590,"end":593},"obj":"Body_part"}],"attributes":[{"id":"A73","pred":"fma_id","subj":"T73","obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"A74","pred":"fma_id","subj":"T74","obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"A75","pred":"fma_id","subj":"T75","obj":"http://purl.org/sig/ont/fma/fma74412"}],"text":"As can be seen in Figure 15, the spectra of the amplification performed inside the chip, and by applying heat with the water bath as well as with the integrated Au-resistive heater, show the same trend as the amplification performed in the Eppendorf and heated by water bath. There is an order of magnitude difference in the fluorescence signal. However, the fluorescence intensity cannot be used as a value to quantify the amount of DNA. EvaGreen is a bis-intercalating cyanine fluorescence dye consisting of two monomeric DNA-binding dyes which are linked by a flexible spacer. These two DNA-binding dyes bind each in between two base pairs, which make them simple and fast, but also nonuniform and non-specific [44,63]. However, with this dye, a simple yes-or-no answer can be obtained if the amplification took place, as can be seen in Figure 15."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T291","span":{"begin":338,"end":344},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T292","span":{"begin":400,"end":401},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T293","span":{"begin":451,"end":452},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T294","span":{"begin":561,"end":562},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T295","span":{"begin":747,"end":748},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"As can be seen in Figure 15, the spectra of the amplification performed inside the chip, and by applying heat with the water bath as well as with the integrated Au-resistive heater, show the same trend as the amplification performed in the Eppendorf and heated by water bath. There is an order of magnitude difference in the fluorescence signal. However, the fluorescence intensity cannot be used as a value to quantify the amount of DNA. EvaGreen is a bis-intercalating cyanine fluorescence dye consisting of two monomeric DNA-binding dyes which are linked by a flexible spacer. These two DNA-binding dyes bind each in between two base pairs, which make them simple and fast, but also nonuniform and non-specific [44,63]. However, with this dye, a simple yes-or-no answer can be obtained if the amplification took place, as can be seen in Figure 15."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T279","span":{"begin":119,"end":124},"obj":"Chemical"},{"id":"T280","span":{"begin":161,"end":163},"obj":"Chemical"},{"id":"T281","span":{"begin":264,"end":269},"obj":"Chemical"},{"id":"T282","span":{"begin":434,"end":437},"obj":"Chemical"},{"id":"T283","span":{"begin":492,"end":495},"obj":"Chemical"},{"id":"T284","span":{"begin":524,"end":527},"obj":"Chemical"},{"id":"T285","span":{"begin":536,"end":540},"obj":"Chemical"},{"id":"T286","span":{"begin":590,"end":593},"obj":"Chemical"},{"id":"T287","span":{"begin":602,"end":606},"obj":"Chemical"},{"id":"T288","span":{"begin":632,"end":636},"obj":"Chemical"},{"id":"T289","span":{"begin":742,"end":745},"obj":"Chemical"}],"attributes":[{"id":"A279","pred":"chebi_id","subj":"T279","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"},{"id":"A280","pred":"chebi_id","subj":"T280","obj":"http://purl.obolibrary.org/obo/CHEBI_29287"},{"id":"A281","pred":"chebi_id","subj":"T281","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"},{"id":"A282","pred":"chebi_id","subj":"T282","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A283","pred":"chebi_id","subj":"T283","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"},{"id":"A284","pred":"chebi_id","subj":"T284","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A285","pred":"chebi_id","subj":"T285","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"},{"id":"A286","pred":"chebi_id","subj":"T286","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A287","pred":"chebi_id","subj":"T287","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"},{"id":"A288","pred":"chebi_id","subj":"T288","obj":"http://purl.obolibrary.org/obo/CHEBI_22695"},{"id":"A289","pred":"chebi_id","subj":"T289","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"}],"text":"As can be seen in Figure 15, the spectra of the amplification performed inside the chip, and by applying heat with the water bath as well as with the integrated Au-resistive heater, show the same trend as the amplification performed in the Eppendorf and heated by water bath. There is an order of magnitude difference in the fluorescence signal. However, the fluorescence intensity cannot be used as a value to quantify the amount of DNA. EvaGreen is a bis-intercalating cyanine fluorescence dye consisting of two monomeric DNA-binding dyes which are linked by a flexible spacer. These two DNA-binding dyes bind each in between two base pairs, which make them simple and fast, but also nonuniform and non-specific [44,63]. However, with this dye, a simple yes-or-no answer can be obtained if the amplification took place, as can be seen in Figure 15."}

{"project":"LitCovid-sentences","denotations":[{"id":"T328","span":{"begin":0,"end":275},"obj":"Sentence"},{"id":"T329","span":{"begin":276,"end":345},"obj":"Sentence"},{"id":"T330","span":{"begin":346,"end":438},"obj":"Sentence"},{"id":"T331","span":{"begin":439,"end":579},"obj":"Sentence"},{"id":"T332","span":{"begin":580,"end":722},"obj":"Sentence"},{"id":"T333","span":{"begin":723,"end":850},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"As can be seen in Figure 15, the spectra of the amplification performed inside the chip, and by applying heat with the water bath as well as with the integrated Au-resistive heater, show the same trend as the amplification performed in the Eppendorf and heated by water bath. There is an order of magnitude difference in the fluorescence signal. However, the fluorescence intensity cannot be used as a value to quantify the amount of DNA. EvaGreen is a bis-intercalating cyanine fluorescence dye consisting of two monomeric DNA-binding dyes which are linked by a flexible spacer. These two DNA-binding dyes bind each in between two base pairs, which make them simple and fast, but also nonuniform and non-specific [44,63]. However, with this dye, a simple yes-or-no answer can be obtained if the amplification took place, as can be seen in Figure 15."}