PMC:7143804 / 38998-39373 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"270","span":{"begin":97,"end":100},"obj":"Gene"},{"id":"271","span":{"begin":54,"end":57},"obj":"Gene"}],"attributes":[{"id":"A270","pred":"tao:has_database_id","subj":"270","obj":"Gene:6236"},{"id":"A271","pred":"tao:has_database_id","subj":"271","obj":"Gene:6236"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"These reactions are carried out in a conventional Bio-Rad CFX96 Touch Real-Time PCR machine (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the results show that the chosen proof-of-principle DNA amplification reaction is temperature dependent to some extent, but that there is a wide range of temperatures at which the amplification can be performed, i.e., 25°C to 35°C."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T63","span":{"begin":196,"end":199},"obj":"Body_part"}],"attributes":[{"id":"A63","pred":"fma_id","subj":"T63","obj":"http://purl.org/sig/ont/fma/fma74412"}],"text":"These reactions are carried out in a conventional Bio-Rad CFX96 Touch Real-Time PCR machine (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the results show that the chosen proof-of-principle DNA amplification reaction is temperature dependent to some extent, but that there is a wide range of temperatures at which the amplification can be performed, i.e., 25°C to 35°C."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T263","span":{"begin":35,"end":36},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T264","span":{"begin":282,"end":283},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T265","span":{"begin":370,"end":372},"obj":"http://purl.obolibrary.org/obo/CLO_0001000"}],"text":"These reactions are carried out in a conventional Bio-Rad CFX96 Touch Real-Time PCR machine (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the results show that the chosen proof-of-principle DNA amplification reaction is temperature dependent to some extent, but that there is a wide range of temperatures at which the amplification can be performed, i.e., 25°C to 35°C."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T244","span":{"begin":196,"end":199},"obj":"Chemical"}],"attributes":[{"id":"A244","pred":"chebi_id","subj":"T244","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"}],"text":"These reactions are carried out in a conventional Bio-Rad CFX96 Touch Real-Time PCR machine (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the results show that the chosen proof-of-principle DNA amplification reaction is temperature dependent to some extent, but that there is a wide range of temperatures at which the amplification can be performed, i.e., 25°C to 35°C."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T15","span":{"begin":196,"end":213},"obj":"http://purl.obolibrary.org/obo/GO_0006277"}],"text":"These reactions are carried out in a conventional Bio-Rad CFX96 Touch Real-Time PCR machine (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the results show that the chosen proof-of-principle DNA amplification reaction is temperature dependent to some extent, but that there is a wide range of temperatures at which the amplification can be performed, i.e., 25°C to 35°C."}

{"project":"LitCovid-sentences","denotations":[{"id":"T303","span":{"begin":0,"end":375},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"These reactions are carried out in a conventional Bio-Rad CFX96 Touch Real-Time PCR machine (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the results show that the chosen proof-of-principle DNA amplification reaction is temperature dependent to some extent, but that there is a wide range of temperatures at which the amplification can be performed, i.e., 25°C to 35°C."}