PMC:7143804 / 38512-39489
Annnotations
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 270 | 583-586 | Gene | denotes | Rad | Gene:6236 |
| 271 | 540-543 | Gene | denotes | Rad | Gene:6236 |
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T59 | 133-135 | Body_part | denotes | V2 | http://purl.org/sig/ont/fma/fma13443|http://purl.org/sig/ont/fma/fma68615 |
| T61 | 136-139 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
| T62 | 289-296 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T63 | 682-685 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T258 | 257-259 | http://purl.obolibrary.org/obo/CLO_0001000 | denotes | 35 |
| T259 | 297-305 | http://purl.obolibrary.org/obo/CLO_0001658 | denotes | activity |
| T260 | 321-322 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T261 | 353-354 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T262 | 381-387 | http://purl.obolibrary.org/obo/SO_0000418 | denotes | signal |
| T263 | 521-522 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T264 | 768-769 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T265 | 856-858 | http://purl.obolibrary.org/obo/CLO_0001000 | denotes | 35 |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T239 | 57-60 | Chemical | denotes | MDA | http://purl.obolibrary.org/obo/CHEBI_566274 |
| T240 | 136-139 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T241 | 184-187 | Chemical | denotes | dye | http://purl.obolibrary.org/obo/CHEBI_37958 |
| T242 | 289-296 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T243 | 395-398 | Chemical | denotes | MDA | http://purl.obolibrary.org/obo/CHEBI_566274 |
| T244 | 682-685 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T13 | 136-153 | http://purl.obolibrary.org/obo/GO_0006277 | denotes | DNA amplification |
| T14 | 270-281 | http://purl.obolibrary.org/obo/GO_0009056 | denotes | degradation |
| T15 | 682-699 | http://purl.obolibrary.org/obo/GO_0006277 | denotes | DNA amplification |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T300 | 0-188 | Sentence | denotes | First, to determine the temperature window of operation, MDA reactions are performed at 25 °C and 30 °C using the Illustra GenomiPhi V2 DNA amplification kit and EvaGreen fluorescence dye. |
| T301 | 189-338 | Sentence | denotes | From the literature, we know that this reaction does not work above 35 °C due to degradation of the protein activity in presence of a substrate [44]. |
| T302 | 339-485 | Sentence | denotes | In Figure 13, a graph of the fluorescence signal during MDA reactions at 25 °C and 30 °C, together with their non template control (NTC) is shown. |
| T303 | 486-861 | Sentence | denotes | These reactions are carried out in a conventional Bio-Rad CFX96 Touch Real-Time PCR machine (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and the results show that the chosen proof-of-principle DNA amplification reaction is temperature dependent to some extent, but that there is a wide range of temperatures at which the amplification can be performed, i.e., 25°C to 35°C. |
| T304 | 862-977 | Sentence | denotes | This makes the functioning of the integrated resistive heater less critical than the stability shown in Figure 11c. |