PMC:7143804 / 13771-14219 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T33","span":{"begin":222,"end":224},"obj":"Body_part"},{"id":"T35","span":{"begin":225,"end":228},"obj":"Body_part"}],"attributes":[{"id":"A33","pred":"fma_id","subj":"T33","obj":"http://purl.org/sig/ont/fma/fma13443"},{"id":"A34","pred":"fma_id","subj":"T33","obj":"http://purl.org/sig/ont/fma/fma68615"},{"id":"A35","pred":"fma_id","subj":"T35","obj":"http://purl.org/sig/ont/fma/fma74412"}],"text":"The reaction chamber is based on the work of Bruijns et al. [36] and its dimensions are chosen in such way that the internal volume of the reaction chamber is the same as the reaction volume of the used Illustra GenomiPhi V2 DNA amplification kit (GE Healthcare Life Sciences, Eindhoven, The Netherlands) together with the EvaGreen dye solution (Biotium, Fremont, CA, USA), while maintaining an as low as possible surface-area-to-volume ratio [44]."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T95","span":{"begin":61,"end":63},"obj":"http://purl.obolibrary.org/obo/CLO_0001313"}],"text":"The reaction chamber is based on the work of Bruijns et al. [36] and its dimensions are chosen in such way that the internal volume of the reaction chamber is the same as the reaction volume of the used Illustra GenomiPhi V2 DNA amplification kit (GE Healthcare Life Sciences, Eindhoven, The Netherlands) together with the EvaGreen dye solution (Biotium, Fremont, CA, USA), while maintaining an as low as possible surface-area-to-volume ratio [44]."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T84","span":{"begin":225,"end":228},"obj":"Chemical"},{"id":"T85","span":{"begin":248,"end":250},"obj":"Chemical"},{"id":"T86","span":{"begin":332,"end":335},"obj":"Chemical"},{"id":"T87","span":{"begin":336,"end":344},"obj":"Chemical"}],"attributes":[{"id":"A84","pred":"chebi_id","subj":"T84","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A85","pred":"chebi_id","subj":"T85","obj":"http://purl.obolibrary.org/obo/CHEBI_73801"},{"id":"A86","pred":"chebi_id","subj":"T86","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"},{"id":"A87","pred":"chebi_id","subj":"T87","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"}],"text":"The reaction chamber is based on the work of Bruijns et al. [36] and its dimensions are chosen in such way that the internal volume of the reaction chamber is the same as the reaction volume of the used Illustra GenomiPhi V2 DNA amplification kit (GE Healthcare Life Sciences, Eindhoven, The Netherlands) together with the EvaGreen dye solution (Biotium, Fremont, CA, USA), while maintaining an as low as possible surface-area-to-volume ratio [44]."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T7","span":{"begin":225,"end":242},"obj":"http://purl.obolibrary.org/obo/GO_0006277"}],"text":"The reaction chamber is based on the work of Bruijns et al. [36] and its dimensions are chosen in such way that the internal volume of the reaction chamber is the same as the reaction volume of the used Illustra GenomiPhi V2 DNA amplification kit (GE Healthcare Life Sciences, Eindhoven, The Netherlands) together with the EvaGreen dye solution (Biotium, Fremont, CA, USA), while maintaining an as low as possible surface-area-to-volume ratio [44]."}

{"project":"LitCovid-sentences","denotations":[{"id":"T117","span":{"begin":0,"end":448},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The reaction chamber is based on the work of Bruijns et al. [36] and its dimensions are chosen in such way that the internal volume of the reaction chamber is the same as the reaction volume of the used Illustra GenomiPhi V2 DNA amplification kit (GE Healthcare Life Sciences, Eindhoven, The Netherlands) together with the EvaGreen dye solution (Biotium, Fremont, CA, USA), while maintaining an as low as possible surface-area-to-volume ratio [44]."}