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PMC:7143804 / 12767-13453 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T32 585-588 Body_part denotes DNA http://purl.org/sig/ont/fma/fma74412

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T88 128-129 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T89 294-297 http://purl.obolibrary.org/obo/CLO_0051582 denotes has
T90 533-534 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T91 678-685 http://purl.obolibrary.org/obo/OBI_0000968 denotes devices

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T78 12-15 Chemical denotes MDA http://purl.obolibrary.org/obo/CHEBI_566274
T79 130-136 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T80 585-588 Chemical denotes DNA http://purl.obolibrary.org/obo/CHEBI_16991
T81 601-604 Chemical denotes MDA http://purl.obolibrary.org/obo/CHEBI_566274

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T106 0-244 Sentence denotes Even though MDA is considered an isothermal process, prior to the reaction and to the addition of most reactants, the dsDNA and a buffer are heated up to 95 °C to denature the dsDNA to ssDNA and to give hexamers the initial access to the ssDNA.
T107 245-325 Sentence denotes After the amplification reaction, the polymerase has to be inactivated at 65 °C.
T108 326-469 Sentence denotes However, this does not require fast temperature changes, as would be the case with, for example, the temperature cycling in PCR amplifications.
T109 470-686 Sentence denotes This, together with the robustness of the amplification (it is a self-limiting reaction that amplifies all present DNA [50]) makes MDA perfectly suitable as proof-of-principle amplification reaction for such devices.

2_test

Id Subject Object Predicate Lexical cue
32106462-16786504-69893313 590-592 16786504 denotes 50