PMC:7120874 / 11424-11847 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7120874","sourcedb":"PMC","sourceid":"7120874","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7120874","text":"Denaturation of Serum Proteins\nThaw serum from −70°C freezer immediately before serum fractionation.\nAliquot 20 µL of serum to each well in a 96-well sample plate.\nAdd 30 µL of D1 denaturing buffer to the well containing the serum to denature the serum proteins.\nShake the sample plate on a Micromix 5-01 shaker vigorously (in an amplitude of 7 and form of 20 Hg) for 20 min at 4°C to mix the serum with the D1 buffer well.","divisions":[{"label":"title","span":{"begin":0,"end":30}},{"label":"p","span":{"begin":31,"end":100}},{"label":"p","span":{"begin":101,"end":163}},{"label":"p","span":{"begin":164,"end":262}}],"tracks":[]}