PMC:7115396 / 9337-10593 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	fma_id
T44	57-61	Body_part	denotes	gene	http://purl.org/sig/ont/fma/fma74402
T45	273-277	Body_part	denotes	gene	http://purl.org/sig/ont/fma/fma74402
T46	643-647	Body_part	denotes	gene	http://purl.org/sig/ont/fma/fma74402
T47	765-771	Body_part	denotes	genome	http://purl.org/sig/ont/fma/fma84116
T48	955-965	Body_part	denotes	nucleotide	http://purl.org/sig/ont/fma/fma82740
T49	1094-1098	Body_part	denotes	gene	http://purl.org/sig/ont/fma/fma74402

Id	Subject	Object	Predicate	Lexical cue	uberon_id
T11	968-972	Body_part	denotes	tail	http://purl.obolibrary.org/obo/UBERON_0002415

Id	Subject	Object	Predicate	Lexical cue
T120	57-61	http://purl.obolibrary.org/obo/OGG_0000000002	denotes	gene
T121	80-81	http://purl.obolibrary.org/obo/CLO_0001020	denotes	a
T122	108-110	http://purl.obolibrary.org/obo/CLO_0050050	denotes	S1
T123	116-117	http://purl.obolibrary.org/obo/CLO_0001020	denotes	A
T124	143-145	http://purl.obolibrary.org/obo/CLO_0050050	denotes	S1
T125	273-277	http://purl.obolibrary.org/obo/OGG_0000000002	denotes	gene
T126	440-442	http://purl.obolibrary.org/obo/CLO_0050050	denotes	S1
T127	534-536	http://purl.obolibrary.org/obo/CLO_0008922	denotes	S2
T128	534-536	http://purl.obolibrary.org/obo/CLO_0050052	denotes	S2
T129	630-631	http://purl.obolibrary.org/obo/CLO_0001020	denotes	a
T130	643-647	http://purl.obolibrary.org/obo/OGG_0000000002	denotes	gene
T131	650-651	http://purl.obolibrary.org/obo/CLO_0001021	denotes	B
T132	719-721	http://purl.obolibrary.org/obo/CLO_0050050	denotes	S1
T133	726-729	http://purl.obolibrary.org/obo/CLO_0050884	denotes	Ten
T134	852-861	http://purl.obolibrary.org/obo/UBERON_0007651	denotes	junctions
T135	882-883	http://purl.obolibrary.org/obo/CLO_0001020	denotes	a
T136	941-944	http://purl.obolibrary.org/obo/CLO_0009364	denotes	TM1
T137	950-951	http://purl.obolibrary.org/obo/CLO_0001020	denotes	a
T138	968-972	http://purl.obolibrary.org/obo/UBERON_0002415	denotes	tail
T139	1094-1098	http://purl.obolibrary.org/obo/OGG_0000000002	denotes	gene
T140	1166-1168	http://purl.obolibrary.org/obo/CLO_0050050	denotes	S1

Id	Subject	Object	Predicate	Lexical cue	chebi_id
T23	534-536	Chemical	denotes	S2	http://purl.obolibrary.org/obo/CHEBI_29387
T24	955-965	Chemical	denotes	nucleotide	http://purl.obolibrary.org/obo/CHEBI_36976

Id	Subject	Object	Predicate	Lexical cue
T58	226-392	Sentence	denotes	The plasmids pIBYZS and pH120S contained the S gene of IBYZ strain and H120 strain, respectively, were constructed during the establishment of reverse genetic system.
T59	393-561	Sentence	denotes	Primers PS1F and PS1R were used to amplify the S1 fragment of IBYZ and vector fragment, while primers PS2F and PS2R were used to amplify the S2 fragment of H120 strain.
T60	562-725	Sentence	denotes	By overlapping PCR, the pYZS1H120S2 was constructed which contained a chimeric S gene. (B) Strategy for the construction of full-length cDNA clones of rH120-S1/YZ.
T61	726-811	Sentence	denotes	Ten cDNA fragments covering the entire genome of H120 strain was amplified by RT-PCR.
T62	812-1014	Sentence	denotes	Unique Bsa I sites were inserted at the junctions between each clone, a unique T7 start site was inserted at the 5′ end of clone TM1, and a 28-nucleotide T tail was inserted at the 3′ end of clone TM10.
T63	1015-1099	Sentence	denotes	The S fragment in the pMDTM8 plasmid of H120 strain was replaced by chimeric S gene.
T64	1100-1256	Sentence	denotes	By using appropriate ligation strategy, the genomic cDNA of rH120-S1/YZ was assembled by in vitro ligation using appropriate restriction sites as indicated.

Id	Subject	Object	Predicate	Lexical cue
126	188-192	Chemical	denotes	IBYZ
127	213-224	Chemical	denotes	pYZS1H120S2