PMC:7115396 / 16254-16952 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T77","span":{"begin":278,"end":281},"obj":"Body_part"},{"id":"T78","span":{"begin":617,"end":620},"obj":"Body_part"}],"attributes":[{"id":"A77","pred":"fma_id","subj":"T77","obj":"http://purl.org/sig/ont/fma/fma54448"},{"id":"A78","pred":"fma_id","subj":"T78","obj":"http://purl.org/sig/ont/fma/fma67095"}],"text":"To determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively. 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. . Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C. After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T21","span":{"begin":278,"end":281},"obj":"Body_part"}],"attributes":[{"id":"A21","pred":"uberon_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/UBERON_0000970"}],"text":"To determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively. 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. . Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C. After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T214","span":{"begin":186,"end":188},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T215","span":{"begin":278,"end":281},"obj":"http://www.ebi.ac.uk/efo/EFO_0000827"},{"id":"T216","span":{"begin":392,"end":394},"obj":"http://purl.obolibrary.org/obo/CLO_0050510"},{"id":"T217","span":{"begin":396,"end":398},"obj":"http://purl.obolibrary.org/obo/CLO_0050507"},{"id":"T218","span":{"begin":544,"end":549},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"}],"text":"To determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively. 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. . Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C. After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T43","span":{"begin":85,"end":90},"obj":"Chemical"},{"id":"T44","span":{"begin":268,"end":273},"obj":"Chemical"}],"attributes":[{"id":"A43","pred":"chebi_id","subj":"T43","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A44","pred":"chebi_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"}],"text":"To determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively. 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. . Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C. After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T21","span":{"begin":26,"end":40},"obj":"http://purl.obolibrary.org/obo/GO_0019076"},{"id":"T22","span":{"begin":633,"end":654},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T23","span":{"begin":641,"end":654},"obj":"http://purl.obolibrary.org/obo/GO_0006351"}],"text":"To determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively. 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. . Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C. After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously."}

{"project":"LitCovid-sentences","denotations":[{"id":"T111","span":{"begin":0,"end":214},"obj":"Sentence"},{"id":"T112","span":{"begin":215,"end":314},"obj":"Sentence"},{"id":"T113","span":{"begin":315,"end":436},"obj":"Sentence"},{"id":"T114","span":{"begin":437,"end":698},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively. 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. . Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C. After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously."}

{"project":"LitCovid-PD-HP","denotations":[{"id":"T12","span":{"begin":278,"end":286},"obj":"Phenotype"}],"attributes":[{"id":"A12","pred":"hp_id","subj":"T12","obj":"http://purl.obolibrary.org/obo/HP_0000508"}],"text":"To determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively. 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. . Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C. After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously."}

{"project":"LitCovid-PubTator","denotations":[{"id":"225","span":{"begin":230,"end":235},"obj":"Species"},{"id":"226","span":{"begin":222,"end":225},"obj":"Chemical"},{"id":"227","span":{"begin":378,"end":381},"obj":"Chemical"}],"attributes":[{"id":"A225","pred":"tao:has_database_id","subj":"225","obj":"Tax:9031"},{"id":"A226","pred":"tao:has_database_id","subj":"226","obj":"MESH:D007854"},{"id":"A227","pred":"tao:has_database_id","subj":"227","obj":"MESH:D007854"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"To determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively. 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. . Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C. After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously."}