PMC:7115396 / 16232-16952 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7115396","sourcedb":"PMC","sourceid":"7115396","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7115396","text":"2.7.4 Viral shedding\nTo determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively. 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. . Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C. After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described 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