PMC:7115396 / 13641-16952 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7115396","sourcedb":"PMC","sourceid":"7115396","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7115396","text":"2.7 Safety testing\n\n2.7.1 Assessment of pathogenicity in one-day-old SPF chickens\nThe chickens were separately housed in isolators under consistent conditions, and food and water was provided ad libitum. A total of 120 one-day-old SPF chickens were randomly divided into four groups (n = 30 per group). The chickens in the experimental groups were intranasally inoculated with 200 μL allantoic fluid per chick containing 105EID50 of the rH120, rIBYZ, and rH120-S1/YZ strains. The control group (n = 30) was inoculated with 200 μL sterile PBS via the same route. The morbidity and mortality was followed-up for 14 days. All experimental groups were monitored daily for clinical signs related to IB infection for 14 days post-infection (dpi), including coughing, sneezing, and tracheal rales. Dead chickens were examined for gross tracheal, lung, and kidney lesions.\n\n2.7.2 Tissue tropism of early viral infection in vivo\nA total of 100 1-day-old SPF chickens were assigned to four groups (n = 25 per group). The birds were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively via eye drop and the intranasal route and 200 μL PBS per chick was administered to the control group (n = 25) via the same route. The tracheas, lungs, kidneys, and bursa from the 5 inoculated birds per group were harvested at 1, 3, 5, and 7 dpi, weighed, and collected into 1 mL PBS per sample and frozen at −80 °C. After grinding the samples, the viral RNA was extracted using TRIzol, and the cDNA was obtained by reverse transcription using a PrimeScript RT Master Mix Perfect Real Time Kit (TaKaRa, Otsu, Shiga, Japan). The viral RNA copies from each of the different samples were detected by real-time PCR as described above. All assays were run in triplicate and the copy number for each virus was calculated according to the standard curve.\n\n2.7.3 Histopathology\nAfter inoculation with 105 EID50 dose of the rH120, rIBYZ, and rH120-S1/YZ strains, three infected chickens per group were randomly selected to be euthanized by bleeding, and the tissue pathology was examined in the different groups at 3, 5, 7, 10 dpi. The tracheas, lungs, kidneys, and bursas of three dead or other randomly-selected chickens from the experimental and control groups (inoculated with PBS) were collected at 5 or 7 dpi and further processed for histopathology. The samples were fixed in formalin, embedded in paraffin, cut into 5 μm sections, and stained with hematoxylin and eosin. The slides were examined under light microscopy for the presence of lesions.\n\n2.7.4 Viral shedding\nTo determine the level of viral shedding of the different recombinants, 10 birds per group were inoculated with 200 μL allantoic fluid containing 105 EID50 of the rH120, rIBYZ, or rH120-S1/YZ strains, respectively. 200 μL PBS per chick was administered to the control group via eye drop and the intranasal route. . Oral and cloacal swabs were collected from each bird into 1 mL PBS at 7, 14, 18, 22, 26, and 30 dpi and stored at −80 °C. After freezing and thawing three times and centrifuging at 8,000 × g for 5 min at 4 °C, relative amount of virus present in 200 μL of supernatants of each sample was quantified by RNA extraction, reverse transcription, and real-time PCR as described previously.","divisions":[{"label":"label","span":{"begin":0,"end":3}},{"label":"title","span":{"begin":5,"end":19}},{"label":"sec","span":{"begin":21,"end":866}},{"label":"label","span":{"begin":21,"end":26}},{"label":"title","span":{"begin":28,"end":83}},{"label":"p","span":{"begin":84,"end":866}},{"label":"sec","span":{"begin":868,"end":1889}},{"label":"label","span":{"begin":868,"end":873}},{"label":"title","span":{"begin":875,"end":922}},{"label":"p","span":{"begin":923,"end":1889}},{"label":"sec","span":{"begin":1891,"end":2589}},{"label":"label","span":{"begin":1891,"end":1896}},{"label":"title","span":{"begin":1898,"end":1912}},{"label":"p","span":{"begin":1913,"end":2589}},{"label":"label","span":{"begin":2591,"end":2596}},{"label":"title","span":{"begin":2598,"end":2612}}],"tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T63","span":{"begin":841,"end":845},"obj":"Body_part"},{"id":"T64","span":{"begin":851,"end":857},"obj":"Body_part"},{"id":"T65","span":{"begin":875,"end":881},"obj":"Body_part"},{"id":"T66","span":{"begin":1147,"end":1150},"obj":"Body_part"},{"id":"T67","span":{"begin":1277,"end":1285},"obj":"Body_part"},{"id":"T68","span":{"begin":1287,"end":1292},"obj":"Body_part"},{"id":"T69","span":{"begin":1294,"end":1301},"obj":"Body_part"},{"id":"T70","span":{"begin":1307,"end":1312},"obj":"Body_part"},{"id":"T71","span":{"begin":1497,"end":1500},"obj":"Body_part"},{"id":"T72","span":{"begin":1676,"end":1679},"obj":"Body_part"},{"id":"T73","span":{"begin":2092,"end":2098},"obj":"Body_part"},{"id":"T74","span":{"begin":2170,"end":2178},"obj":"Body_part"},{"id":"T75","span":{"begin":2180,"end":2185},"obj":"Body_part"},{"id":"T76","span":{"begin":2187,"end":2194},"obj":"Body_part"},{"id":"T77","span":{"begin":2891,"end":2894},"obj":"Body_part"},{"id":"T78","span":{"begin":3230,"end":3233},"obj":"Body_part"}],"attributes":[{"id":"A63","pred":"fma_id","subj":"T63","obj":"http://purl.org/sig/ont/fma/fma7195"},{"id":"A64","pred":"fma_id","subj":"T64","obj":"http://purl.org/sig/ont/fma/fma7203"},{"id":"A65","pred":"fma_id","subj":"T65","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A66","pred":"fma_id","subj":"T66","obj":"http://purl.org/sig/ont/fma/fma54448"},{"id":"A67","pred":"fma_id","subj":"T67","obj":"http://purl.org/sig/ont/fma/fma7394"},{"id":"A68","pred":"fma_id","subj":"T68","obj":"http://purl.org/sig/ont/fma/fma68877"},{"id":"A69","pred":"fma_id","subj":"T69","obj":"http://purl.org/sig/ont/fma/fma7203"},{"id":"A70","pred":"fma_id","subj":"T70","obj":"http://purl.org/sig/ont/fma/fma9692"},{"id":"A71","pred":"fma_id","subj":"T71","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A72","pred":"fma_id","subj":"T72","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A73","pred":"fma_id","subj":"T73","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A74","pred":"fma_id","subj":"T74","obj":"http://purl.org/sig/ont/fma/fma7394"},{"id":"A75","pred":"fma_id","subj":"T75","obj":"http://purl.org/sig/ont/fma/fma68877"},{"id":"A76","pred":"fma_id","subj":"T76","obj":"http://purl.org/sig/ont/fma/fma7203"},{"id":"A77","pred":"fma_id","subj":"T77","obj":"http://purl.org/sig/ont/fma/fma54448"},{"id":"A78","pred":"fma_id","subj":"T78","obj":"http://purl.org/sig/ont/fma/fma67095"},{"subj":"T63","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T64","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T65","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T66","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T67","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T68","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T69","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T70","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T71","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T72","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T73","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T74","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T75","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T76","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T77","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T78","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T16","span":{"begin":841,"end":845},"obj":"Body_part"},{"id":"T17","span":{"begin":851,"end":857},"obj":"Body_part"},{"id":"T18","span":{"begin":875,"end":881},"obj":"Body_part"},{"id":"T19","span":{"begin":1147,"end":1150},"obj":"Body_part"},{"id":"T20","span":{"begin":2092,"end":2098},"obj":"Body_part"},{"id":"T21","span":{"begin":2891,"end":2894},"obj":"Body_part"}],"attributes":[{"id":"A16","pred":"uberon_id","subj":"T16","obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"A17","pred":"uberon_id","subj":"T17","obj":"http://purl.obolibrary.org/obo/UBERON_0002113"},{"id":"A18","pred":"uberon_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A19","pred":"uberon_id","subj":"T19","obj":"http://purl.obolibrary.org/obo/UBERON_0000970"},{"id":"A20","pred":"uberon_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A21","pred":"uberon_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/UBERON_0000970"},{"subj":"T16","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T17","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T18","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T19","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T20","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T21","pred":"source","obj":"LitCovid-PD-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T24","span":{"begin":532,"end":539},"obj":"Disease"},{"id":"T25","span":{"begin":699,"end":708},"obj":"Disease"},{"id":"T26","span":{"begin":726,"end":735},"obj":"Disease"},{"id":"T27","span":{"begin":899,"end":914},"obj":"Disease"},{"id":"T28","span":{"begin":905,"end":914},"obj":"Disease"}],"attributes":[{"id":"A24","pred":"mondo_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/MONDO_0005047"},{"id":"A25","pred":"mondo_id","subj":"T25","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A26","pred":"mondo_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A27","pred":"mondo_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/MONDO_0005108"},{"id":"A28","pred":"mondo_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"subj":"T24","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T25","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T26","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T27","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T28","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T181","span":{"begin":12,"end":19},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T182","span":{"begin":75,"end":83},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T183","span":{"begin":88,"end":96},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T184","span":{"begin":206,"end":207},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T185","span":{"begin":237,"end":245},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T186","span":{"begin":309,"end":320},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T187","span":{"begin":463,"end":465},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T188","span":{"begin":798,"end":806},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T189","span":{"begin":841,"end":845},"obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"T190","span":{"begin":841,"end":845},"obj":"http://www.ebi.ac.uk/efo/EFO_0000934"},{"id":"T191","span":{"begin":851,"end":857},"obj":"http://purl.obolibrary.org/obo/UBERON_0002113"},{"id":"T192","span":{"begin":851,"end":857},"obj":"http://www.ebi.ac.uk/efo/EFO_0000927"},{"id":"T193","span":{"begin":851,"end":857},"obj":"http://www.ebi.ac.uk/efo/EFO_0000929"},{"id":"T194","span":{"begin":923,"end":924},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T195","span":{"begin":952,"end":960},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T196","span":{"begin":1115,"end":1117},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T197","span":{"begin":1147,"end":1150},"obj":"http://www.ebi.ac.uk/efo/EFO_0000827"},{"id":"T198","span":{"begin":1287,"end":1292},"obj":"http://www.ebi.ac.uk/efo/EFO_0000934"},{"id":"T199","span":{"begin":1294,"end":1301},"obj":"http://purl.obolibrary.org/obo/UBERON_0002113"},{"id":"T200","span":{"begin":1294,"end":1301},"obj":"http://www.ebi.ac.uk/efo/EFO_0000927"},{"id":"T201","span":{"begin":1294,"end":1301},"obj":"http://www.ebi.ac.uk/efo/EFO_0000929"},{"id":"T202","span":{"begin":1366,"end":1370},"obj":"http://purl.obolibrary.org/obo/CLO_0001757"},{"id":"T203","span":{"begin":1372,"end":1376},"obj":"http://purl.obolibrary.org/obo/CLO_0001000"},{"id":"T204","span":{"begin":1586,"end":1587},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T205","span":{"begin":1836,"end":1841},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T206","span":{"begin":1982,"end":1984},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T207","span":{"begin":2012,"end":2020},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T208","span":{"begin":2149,"end":2153},"obj":"http://purl.obolibrary.org/obo/CLO_0001000"},{"id":"T209","span":{"begin":2180,"end":2185},"obj":"http://www.ebi.ac.uk/efo/EFO_0000934"},{"id":"T210","span":{"begin":2187,"end":2194},"obj":"http://purl.obolibrary.org/obo/UBERON_0002113"},{"id":"T211","span":{"begin":2187,"end":2194},"obj":"http://www.ebi.ac.uk/efo/EFO_0000927"},{"id":"T212","span":{"begin":2187,"end":2194},"obj":"http://www.ebi.ac.uk/efo/EFO_0000929"},{"id":"T213","span":{"begin":2248,"end":2256},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9031"},{"id":"T214","span":{"begin":2799,"end":2801},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T215","span":{"begin":2891,"end":2894},"obj":"http://www.ebi.ac.uk/efo/EFO_0000827"},{"id":"T216","span":{"begin":3005,"end":3007},"obj":"http://purl.obolibrary.org/obo/CLO_0050510"},{"id":"T217","span":{"begin":3009,"end":3011},"obj":"http://purl.obolibrary.org/obo/CLO_0050507"},{"id":"T218","span":{"begin":3157,"end":3162},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"}],"attributes":[{"subj":"T181","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T182","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T183","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T184","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T185","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T186","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T187","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T188","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T189","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T190","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T191","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T192","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T193","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T194","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T195","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T196","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T197","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T198","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T199","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T200","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T201","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T202","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T203","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T204","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T205","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T206","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T207","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T208","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T209","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T210","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T211","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T212","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T213","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T214","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T215","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T216","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T217","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T218","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T32","span":{"begin":71,"end":74},"obj":"Chemical"},{"id":"T33","span":{"begin":175,"end":180},"obj":"Chemical"},{"id":"T34","span":{"begin":233,"end":236},"obj":"Chemical"},{"id":"T35","span":{"begin":297,"end":302},"obj":"Chemical"},{"id":"T36","span":{"begin":490,"end":495},"obj":"Chemical"},{"id":"T37","span":{"begin":948,"end":951},"obj":"Chemical"},{"id":"T38","span":{"begin":1002,"end":1007},"obj":"Chemical"},{"id":"T39","span":{"begin":1238,"end":1243},"obj":"Chemical"},{"id":"T40","span":{"begin":1345,"end":1350},"obj":"Chemical"},{"id":"T41","span":{"begin":2025,"end":2030},"obj":"Chemical"},{"id":"T42","span":{"begin":2490,"end":2501},"obj":"Chemical"},{"id":"T43","span":{"begin":2698,"end":2703},"obj":"Chemical"},{"id":"T44","span":{"begin":2881,"end":2886},"obj":"Chemical"}],"attributes":[{"id":"A32","pred":"chebi_id","subj":"T32","obj":"http://purl.obolibrary.org/obo/CHEBI_88542"},{"id":"A33","pred":"chebi_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"},{"id":"A34","pred":"chebi_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/CHEBI_88542"},{"id":"A35","pred":"chebi_id","subj":"T35","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A36","pred":"chebi_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A37","pred":"chebi_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/CHEBI_88542"},{"id":"A38","pred":"chebi_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A39","pred":"chebi_id","subj":"T39","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A40","pred":"chebi_id","subj":"T40","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A41","pred":"chebi_id","subj":"T41","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A42","pred":"chebi_id","subj":"T42","obj":"http://purl.obolibrary.org/obo/CHEBI_51686"},{"id":"A43","pred":"chebi_id","subj":"T43","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A44","pred":"chebi_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"subj":"T32","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T33","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T34","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T35","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T36","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T37","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T38","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T39","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T40","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T41","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T42","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T43","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T44","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T16","span":{"begin":882,"end":889},"obj":"http://purl.obolibrary.org/obo/GO_0009606"},{"id":"T17","span":{"begin":899,"end":914},"obj":"http://purl.obolibrary.org/obo/GO_0016032"},{"id":"T18","span":{"begin":1558,"end":1579},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T19","span":{"begin":1566,"end":1579},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T20","span":{"begin":2598,"end":2612},"obj":"http://purl.obolibrary.org/obo/GO_0019076"},{"id":"T21","span":{"begin":2639,"end":2653},"obj":"http://purl.obolibrary.org/obo/GO_0019076"},{"id":"T22","span":{"begin":3246,"end":3267},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T23","span":{"begin":3254,"end":3267},"obj":"http://purl.obolibrary.org/obo/GO_0006351"}],"attributes":[{"subj":"T16","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T17","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T18","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T19","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T20","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T21","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T22","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T23","pred":"source","obj":"LitCovid-PD-GO-BP"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T89","span":{"begin":0,"end":19},"obj":"Sentence"},{"id":"T90","span":{"begin":21,"end":83},"obj":"Sentence"},{"id":"T91","span":{"begin":84,"end":205},"obj":"Sentence"},{"id":"T92","span":{"begin":206,"end":304},"obj":"Sentence"},{"id":"T93","span":{"begin":305,"end":477},"obj":"Sentence"},{"id":"T94","span":{"begin":478,"end":563},"obj":"Sentence"},{"id":"T95","span":{"begin":564,"end":620},"obj":"Sentence"},{"id":"T96","span":{"begin":621,"end":792},"obj":"Sentence"},{"id":"T97","span":{"begin":793,"end":866},"obj":"Sentence"},{"id":"T98","span":{"begin":868,"end":922},"obj":"Sentence"},{"id":"T99","span":{"begin":923,"end":1009},"obj":"Sentence"},{"id":"T100","span":{"begin":1010,"end":1272},"obj":"Sentence"},{"id":"T101","span":{"begin":1273,"end":1458},"obj":"Sentence"},{"id":"T102","span":{"begin":1459,"end":1665},"obj":"Sentence"},{"id":"T103","span":{"begin":1666,"end":1772},"obj":"Sentence"},{"id":"T104","span":{"begin":1773,"end":1889},"obj":"Sentence"},{"id":"T105","span":{"begin":1891,"end":1912},"obj":"Sentence"},{"id":"T106","span":{"begin":1913,"end":2165},"obj":"Sentence"},{"id":"T107","span":{"begin":2166,"end":2390},"obj":"Sentence"},{"id":"T108","span":{"begin":2391,"end":2512},"obj":"Sentence"},{"id":"T109","span":{"begin":2513,"end":2589},"obj":"Sentence"},{"id":"T110","span":{"begin":2591,"end":2612},"obj":"Sentence"},{"id":"T111","span":{"begin":2613,"end":2827},"obj":"Sentence"},{"id":"T112","span":{"begin":2828,"end":2927},"obj":"Sentence"},{"id":"T113","span":{"begin":2928,"end":3049},"obj":"Sentence"},{"id":"T114","span":{"begin":3050,"end":3311},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T89","pred":"source","obj":"LitCovid-sentences"},{"subj":"T90","pred":"source","obj":"LitCovid-sentences"},{"subj":"T91","pred":"source","obj":"LitCovid-sentences"},{"subj":"T92","pred":"source","obj":"LitCovid-sentences"},{"subj":"T93","pred":"source","obj":"LitCovid-sentences"},{"subj":"T94","pred":"source","obj":"LitCovid-sentences"},{"subj":"T95","pred":"source","obj":"LitCovid-sentences"},{"subj":"T96","pred":"source","obj":"LitCovid-sentences"},{"subj":"T97","pred":"source","obj":"LitCovid-sentences"},{"subj":"T98","pred":"source","obj":"LitCovid-sentences"},{"subj":"T99","pred":"source","obj":"LitCovid-sentences"},{"subj":"T100","pred":"source","obj":"LitCovid-sentences"},{"subj":"T101","pred":"source","obj":"LitCovid-sentences"},{"subj":"T102","pred":"source","obj":"LitCovid-sentences"},{"subj":"T103","pred":"source","obj":"LitCovid-sentences"},{"subj":"T104","pred":"source","obj":"LitCovid-sentences"},{"subj":"T105","pred":"source","obj":"LitCovid-sentences"},{"subj":"T106","pred":"source","obj":"LitCovid-sentences"},{"subj":"T107","pred":"source","obj":"LitCovid-sentences"},{"subj":"T108","pred":"source","obj":"LitCovid-sentences"},{"subj":"T109","pred":"source","obj":"LitCovid-sentences"},{"subj":"T110","pred":"source","obj":"LitCovid-sentences"},{"subj":"T111","pred":"source","obj":"LitCovid-sentences"},{"subj":"T112","pred":"source","obj":"LitCovid-sentences"},{"subj":"T113","pred":"source","obj":"LitCovid-sentences"},{"subj":"T114","pred":"source","obj":"LitCovid-sentences"}]},{"project":"LitCovid-PD-HP","denotations":[{"id":"T9","span":{"begin":753,"end":761},"obj":"Phenotype"},{"id":"T10","span":{"begin":786,"end":791},"obj":"Phenotype"},{"id":"T11","span":{"begin":1147,"end":1155},"obj":"Phenotype"},{"id":"T12","span":{"begin":2891,"end":2899},"obj":"Phenotype"}],"attributes":[{"id":"A9","pred":"hp_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/HP_0012735"},{"id":"A10","pred":"hp_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/HP_0030830"},{"id":"A11","pred":"hp_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/HP_0000508"},{"id":"A12","pred":"hp_id","subj":"T12","obj":"http://purl.obolibrary.org/obo/HP_0000508"},{"subj":"T9","pred":"source","obj":"LitCovid-PD-HP"},{"subj":"T10","pred":"source","obj":"LitCovid-PD-HP"},{"subj":"T11","pred":"source","obj":"LitCovid-PD-HP"},{"subj":"T12","pred":"source","obj":"LitCovid-PD-HP"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"178","span":{"begin":88,"end":96},"obj":"Species"},{"id":"179","span":{"begin":237,"end":245},"obj":"Species"},{"id":"180","span":{"begin":309,"end":317},"obj":"Species"},{"id":"181","span":{"begin":406,"end":411},"obj":"Species"},{"id":"182","span":{"begin":798,"end":806},"obj":"Species"},{"id":"183","span":{"begin":175,"end":180},"obj":"Chemical"},{"id":"184","span":{"begin":540,"end":543},"obj":"Chemical"},{"id":"185","span":{"begin":582,"end":591},"obj":"Disease"},{"id":"186","span":{"begin":696,"end":708},"obj":"Disease"},{"id":"187","span":{"begin":726,"end":735},"obj":"Disease"},{"id":"188","span":{"begin":753,"end":761},"obj":"Disease"},{"id":"189","span":{"begin":851,"end":865},"obj":"Disease"},{"id":"191","span":{"begin":899,"end":914},"obj":"Disease"},{"id":"197","span":{"begin":952,"end":960},"obj":"Species"},{"id":"198","span":{"begin":1200,"end":1205},"obj":"Species"},{"id":"199","span":{"begin":1192,"end":1195},"obj":"Chemical"},{"id":"200","span":{"begin":1422,"end":1425},"obj":"Chemical"},{"id":"201","span":{"begin":1521,"end":1527},"obj":"Chemical"},{"id":"212","span":{"begin":2012,"end":2020},"obj":"Species"},{"id":"213","span":{"begin":2248,"end":2256},"obj":"Species"},{"id":"214","span":{"begin":1965,"end":1970},"obj":"Chemical"},{"id":"215","span":{"begin":2315,"end":2318},"obj":"Chemical"},{"id":"216","span":{"begin":2417,"end":2425},"obj":"Chemical"},{"id":"217","span":{"begin":2439,"end":2447},"obj":"Chemical"},{"id":"218","span":{"begin":2490,"end":2501},"obj":"Chemical"},{"id":"219","span":{"begin":2506,"end":2511},"obj":"Chemical"},{"id":"220","span":{"begin":2003,"end":2011},"obj":"Disease"},{"id":"221","span":{"begin":2074,"end":2082},"obj":"Disease"},{"id":"225","span":{"begin":2843,"end":2848},"obj":"Species"},{"id":"226","span":{"begin":2835,"end":2838},"obj":"Chemical"},{"id":"227","span":{"begin":2991,"end":2994},"obj":"Chemical"}],"attributes":[{"id":"A178","pred":"tao:has_database_id","subj":"178","obj":"Tax:9031"},{"id":"A179","pred":"tao:has_database_id","subj":"179","obj":"Tax:9031"},{"id":"A180","pred":"tao:has_database_id","subj":"180","obj":"Tax:9031"},{"id":"A181","pred":"tao:has_database_id","subj":"181","obj":"Tax:9031"},{"id":"A182","pred":"tao:has_database_id","subj":"182","obj":"Tax:9031"},{"id":"A183","pred":"tao:has_database_id","subj":"183","obj":"MESH:D014867"},{"id":"A184","pred":"tao:has_database_id","subj":"184","obj":"MESH:D007854"},{"id":"A185","pred":"tao:has_database_id","subj":"185","obj":"MESH:D003643"},{"id":"A186","pred":"tao:has_database_id","subj":"186","obj":"MESH:D007239"},{"id":"A187","pred":"tao:has_database_id","subj":"187","obj":"MESH:D007239"},{"id":"A188","pred":"tao:has_database_id","subj":"188","obj":"MESH:D003371"},{"id":"A189","pred":"tao:has_database_id","subj":"189","obj":"MESH:D007674"},{"id":"A191","pred":"tao:has_database_id","subj":"191","obj":"MESH:D001102"},{"id":"A197","pred":"tao:has_database_id","subj":"197","obj":"Tax:9031"},{"id":"A198","pred":"tao:has_database_id","subj":"198","obj":"Tax:9031"},{"id":"A199","pred":"tao:has_database_id","subj":"199","obj":"MESH:D007854"},{"id":"A200","pred":"tao:has_database_id","subj":"200","obj":"MESH:D007854"},{"id":"A201","pred":"tao:has_database_id","subj":"201","obj":"MESH:C411644"},{"id":"A212","pred":"tao:has_database_id","subj":"212","obj":"Tax:9031"},{"id":"A213","pred":"tao:has_database_id","subj":"213","obj":"Tax:9031"},{"id":"A215","pred":"tao:has_database_id","subj":"215","obj":"MESH:D007854"},{"id":"A216","pred":"tao:has_database_id","subj":"216","obj":"MESH:D005557"},{"id":"A217","pred":"tao:has_database_id","subj":"217","obj":"MESH:D010232"},{"id":"A218","pred":"tao:has_database_id","subj":"218","obj":"MESH:D006416"},{"id":"A219","pred":"tao:has_database_id","subj":"219","obj":"MESH:D004801"},{"id":"A220","pred":"tao:has_database_id","subj":"220","obj":"MESH:D007239"},{"id":"A221","pred":"tao:has_database_id","subj":"221","obj":"MESH:D006470"},{"id":"A225","pred":"tao:has_database_id","subj":"225","obj":"Tax:9031"},{"id":"A226","pred":"tao:has_database_id","subj":"226","obj":"MESH:D007854"},{"id":"A227","pred":"tao:has_database_id","subj":"227","obj":"MESH:D007854"},{"subj":"178","pred":"source","obj":"LitCovid-PubTator"},{"subj":"179","pred":"source","obj":"LitCovid-PubTator"},{"subj":"180","pred":"source","obj":"LitCovid-PubTator"},{"subj":"181","pred":"source","obj":"LitCovid-PubTator"},{"subj":"182","pred":"source","obj":"LitCovid-PubTator"},{"subj":"183","pred":"source","obj":"LitCovid-PubTator"},{"subj":"184","pred":"source","obj":"LitCovid-PubTator"},{"subj":"185","pred":"source","obj":"LitCovid-PubTator"},{"subj":"186","pred":"source","obj":"LitCovid-PubTator"},{"subj":"187","pred":"source","obj":"LitCovid-PubTator"},{"subj":"188","pred":"source","obj":"LitCovid-PubTator"},{"subj":"189","pred":"source","obj":"LitCovid-PubTator"},{"subj":"191","pred":"source","obj":"LitCovid-PubTator"},{"subj":"197","pred":"source","obj":"LitCovid-PubTator"},{"subj":"198","pred":"source","obj":"LitCovid-PubTator"},{"subj":"199","pred":"source","obj":"LitCovid-PubTator"},{"subj":"200","pred":"source","obj":"LitCovid-PubTator"},{"subj":"201","pred":"source","obj":"LitCovid-PubTator"},{"subj":"212","pred":"source","obj":"LitCovid-PubTator"},{"subj":"213","pred":"source","obj":"LitCovid-PubTator"},{"subj":"214","pred":"source","obj":"LitCovid-PubTator"},{"subj":"215","pred":"source","obj":"LitCovid-PubTator"},{"subj":"216","pred":"source","obj":"LitCovid-PubTator"},{"subj":"217","pred":"source","obj":"LitCovid-PubTator"},{"subj":"218","pred":"source","obj":"LitCovid-PubTator"},{"subj":"219","pred":"source","obj":"LitCovid-PubTator"},{"subj":"220","pred":"source","obj":"LitCovid-PubTator"},{"subj":"221","pred":"source","obj":"LitCovid-PubTator"},{"subj":"225","pred":"source","obj":"LitCovid-PubTator"},{"subj":"226","pred":"source","obj":"LitCovid-PubTator"},{"subj":"227","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#93ecab","default":true},{"id":"LitCovid-PD-UBERON","color":"#ec9394"},{"id":"LitCovid-PD-MONDO","color":"#93aeec"},{"id":"LitCovid-PD-CLO","color":"#c8ec93"},{"id":"LitCovid-PD-CHEBI","color":"#ec93e2"},{"id":"LitCovid-PD-GO-BP","color":"#93ecdc"},{"id":"LitCovid-sentences","color":"#ecc193"},{"id":"LitCovid-PD-HP","color":"#a793ec"},{"id":"LitCovid-PubTator","color":"#98ec93"}]}]}}