PMC:7115396 / 10963-12770
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T54 | 32-39 | Body_part | denotes | embryos | http://purl.org/sig/ont/fma/fma296970 |
| T55 | 87-94 | Body_part | denotes | embryos | http://purl.org/sig/ont/fma/fma296970 |
| T56 | 903-906 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
| T57 | 1123-1126 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T150 | 24-31 | http://purl.obolibrary.org/obo/NCBITaxon_9031 | denotes | chicken |
| T151 | 32-39 | http://purl.obolibrary.org/obo/UBERON_0000922 | denotes | embryos |
| T152 | 79-86 | http://purl.obolibrary.org/obo/NCBITaxon_9031 | denotes | chicken |
| T153 | 87-94 | http://purl.obolibrary.org/obo/UBERON_0000922 | denotes | embryos |
| T154 | 96-97 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T155 | 493-494 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T156 | 548-549 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | A |
| T157 | 650-654 | http://purl.obolibrary.org/obo/UBERON_0000473 | denotes | test |
| T158 | 769-771 | http://purl.obolibrary.org/obo/CLO_0050510 | denotes | 18 |
| T159 | 799-806 | http://purl.obolibrary.org/obo/NCBITaxon_9031 | denotes | chicken |
| T160 | 874-876 | http://purl.obolibrary.org/obo/CLO_0050050 | denotes | S1 |
| T161 | 883-884 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T162 | 1081-1083 | http://purl.obolibrary.org/obo/CLO_0050510 | denotes | 18 |
| T163 | 1089-1091 | http://purl.obolibrary.org/obo/CLO_0001313 | denotes | 36 |
| T164 | 1093-1095 | http://purl.obolibrary.org/obo/CLO_0001382 | denotes | 48 |
| T165 | 1312-1313 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T166 | 1754-1759 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T26 | 795-798 | Chemical | denotes | SPF | http://purl.obolibrary.org/obo/CHEBI_88542 |
| T27 | 822-827 | Chemical | denotes | group | http://purl.obolibrary.org/obo/CHEBI_24433 |
| T28 | 1068-1073 | Chemical | denotes | group | http://purl.obolibrary.org/obo/CHEBI_24433 |
| T29 | 1520-1522 | Chemical | denotes | II | http://purl.obolibrary.org/obo/CHEBI_74067 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T9 | 5-11 | http://purl.obolibrary.org/obo/GO_0040007 | denotes | Growth |
| T10 | 61-67 | http://purl.obolibrary.org/obo/GO_0040007 | denotes | growth |
| T11 | 108-129 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | reverse transcription |
| T12 | 116-129 | http://purl.obolibrary.org/obo/GO_0006351 | denotes | transcription |
| T13 | 935-941 | http://purl.obolibrary.org/obo/GO_0040007 | denotes | growth |
| T14 | 1284-1305 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | reverse transcription |
| T15 | 1292-1305 | http://purl.obolibrary.org/obo/GO_0006351 | denotes | transcription |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T68 | 0-39 | Sentence | denotes | 2.5 Growth kinetics in chicken embryos |
| T69 | 40-202 | Sentence | denotes | To examine the viral growth ability in chicken embryos, a real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) method was established. |
| T70 | 203-314 | Sentence | denotes | According to the sequences of IBV from GenBank, primers were designed based on conservative area in the 5′-UTR. |
| T71 | 315-425 | Sentence | denotes | The upstream primer was 5′-CCGTTGCTTGGGCTACCTAGT-3′, and the downstream primer was 5′-CGCCTACCGCTAGATGAACC-3′. |
| T72 | 426-547 | Sentence | denotes | The amplification product was cloned to pMD18-T vector (Takara) as a positive plasmid and its concentration was measured. |
| T73 | 548-655 | Sentence | denotes | A gradient dilution of 5 × 102–5 × 108 copies/μL of the plasmid was used as template for quantitation test. |
| T74 | 656-768 | Sentence | denotes | By plotting the cycle threshold (CT) values against the copies of the plasmid, the standard curve was generated. |
| T75 | 769-960 | Sentence | denotes | 18 10-day-old embryonated SPF chicken eggs (six eggs/group) were inoculated with rH120, rIBYZ, and rH120-S1/YZ at a dose of 107 viral RNA copies/100 μL, and used for growth curve experiments. |
| T76 | 961-1122 | Sentence | denotes | The allantoic fluids were collected separately by syringe from the six inoculated embryonated eggs of each group at 12, 18, 24, 36, 48, 72, 96 h per inoculation. |
| T77 | 1123-1391 | Sentence | denotes | RNA was extracted from the allantoic fluids using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. cDNA was obtained by reverse transcription using a PrimeScript RT Master Mix Perfect Real Time Kit (TaKaRa, Otsu, Shiga, Japan). |
| T78 | 1392-1608 | Sentence | denotes | The viral copies were measured by absolute quantitative method of Real-time PCR, which was performed using SYBR® Premix Ex Taq™ II (TaKaRa, Otsu, Shiga, Japan) on an Applied Biosystems 7500 Fast Real-time PCR System. |
| T79 | 1609-1684 | Sentence | denotes | The standard curve was plotted against the log of the template copy number. |
| T80 | 1685-1807 | Sentence | denotes | All of the assays were run in triplicate and the copy number of each virus was calculated according to the standard curve. |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 145 | 24-31 | Species | denotes | chicken | Tax:9031 |
| 148 | 79-86 | Species | denotes | chicken | Tax:9031 |
| 149 | 233-236 | Species | denotes | IBV | Tax:11120 |
| 152 | 799-806 | Species | denotes | chicken | Tax:9031 |
| 153 | 1173-1179 | Chemical | denotes | TRIzol | MESH:C411644 |