PMC:7100515 / 6354-6727 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T43","span":{"begin":31,"end":38},"obj":"Body_part"},{"id":"T44","span":{"begin":66,"end":71},"obj":"Body_part"},{"id":"T45","span":{"begin":111,"end":118},"obj":"Body_part"},{"id":"T46","span":{"begin":188,"end":195},"obj":"Body_part"},{"id":"T47","span":{"begin":238,"end":249},"obj":"Body_part"},{"id":"T48","span":{"begin":264,"end":285},"obj":"Body_part"},{"id":"T49","span":{"begin":287,"end":289},"obj":"Body_part"},{"id":"T50","span":{"begin":317,"end":328},"obj":"Body_part"},{"id":"T51","span":{"begin":343,"end":350},"obj":"Body_part"}],"attributes":[{"id":"A43","pred":"fma_id","subj":"T43","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A44","pred":"fma_id","subj":"T44","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A45","pred":"fma_id","subj":"T45","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A46","pred":"fma_id","subj":"T46","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A47","pred":"fma_id","subj":"T47","obj":"http://purl.org/sig/ont/fma/fma82739"},{"id":"A48","pred":"fma_id","subj":"T48","obj":"http://purl.org/sig/ont/fma/fma63842"},{"id":"A49","pred":"fma_id","subj":"T49","obj":"http://purl.org/sig/ont/fma/fma63842"},{"id":"A50","pred":"fma_id","subj":"T50","obj":"http://purl.org/sig/ont/fma/fma66835"},{"id":"A51","pred":"fma_id","subj":"T51","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"To enhance expression of the S protein of SARS-CoV-2 in mammalian cells, a codon-optimized cDNA encoding the S protein and 3xFLAG tag was synthesized, and to facilitate incorporation of S protein into lentiviral pseudovirons, the last 19 amino acids containing an endoplasmic reticulum (ER)-retention signal from the cytoplasmic tail of the S protein was removed (Fig. 1a)."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T1","span":{"begin":329,"end":333},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0002415"}],"text":"To enhance expression of the S protein of SARS-CoV-2 in mammalian cells, a codon-optimized cDNA encoding the S protein and 3xFLAG tag was synthesized, and to facilitate incorporation of S protein into lentiviral pseudovirons, the last 19 amino acids containing an endoplasmic reticulum (ER)-retention signal from the cytoplasmic tail of the S protein was removed (Fig. 1a)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T57","span":{"begin":42,"end":50},"obj":"Disease"}],"attributes":[{"id":"A57","pred":"mondo_id","subj":"T57","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"To enhance expression of the S protein of SARS-CoV-2 in mammalian cells, a codon-optimized cDNA encoding the S protein and 3xFLAG tag was synthesized, and to facilitate incorporation of S protein into lentiviral pseudovirons, the last 19 amino acids containing an endoplasmic reticulum (ER)-retention signal from the cytoplasmic tail of the S protein was removed (Fig. 1a)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T93","span":{"begin":66,"end":71},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T94","span":{"begin":73,"end":74},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T95","span":{"begin":301,"end":307},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T96","span":{"begin":329,"end":333},"obj":"http://purl.obolibrary.org/obo/UBERON_0002415"}],"text":"To enhance expression of the S protein of SARS-CoV-2 in mammalian cells, a codon-optimized cDNA encoding the S protein and 3xFLAG tag was synthesized, and to facilitate incorporation of S protein into lentiviral pseudovirons, the last 19 amino acids containing an endoplasmic reticulum (ER)-retention signal from the cytoplasmic tail of the S protein was removed (Fig. 1a)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T57","span":{"begin":31,"end":38},"obj":"Chemical"},{"id":"T58","span":{"begin":111,"end":118},"obj":"Chemical"},{"id":"T59","span":{"begin":188,"end":195},"obj":"Chemical"},{"id":"T60","span":{"begin":238,"end":249},"obj":"Chemical"},{"id":"T61","span":{"begin":238,"end":243},"obj":"Chemical"},{"id":"T62","span":{"begin":244,"end":249},"obj":"Chemical"},{"id":"T63","span":{"begin":343,"end":350},"obj":"Chemical"}],"attributes":[{"id":"A57","pred":"chebi_id","subj":"T57","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A58","pred":"chebi_id","subj":"T58","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A59","pred":"chebi_id","subj":"T59","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A60","pred":"chebi_id","subj":"T60","obj":"http://purl.obolibrary.org/obo/CHEBI_33709"},{"id":"A61","pred":"chebi_id","subj":"T61","obj":"http://purl.obolibrary.org/obo/CHEBI_46882"},{"id":"A62","pred":"chebi_id","subj":"T62","obj":"http://purl.obolibrary.org/obo/CHEBI_37527"},{"id":"A63","pred":"chebi_id","subj":"T63","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"To enhance expression of the S protein of SARS-CoV-2 in mammalian cells, a codon-optimized cDNA encoding the S protein and 3xFLAG tag was synthesized, and to facilitate incorporation of S protein into lentiviral pseudovirons, the last 19 amino acids containing an endoplasmic reticulum (ER)-retention signal from the cytoplasmic tail of the S protein was removed (Fig. 1a)."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T8","span":{"begin":291,"end":300},"obj":"http://purl.obolibrary.org/obo/GO_0051235"}],"text":"To enhance expression of the S protein of SARS-CoV-2 in mammalian cells, a codon-optimized cDNA encoding the S protein and 3xFLAG tag was synthesized, and to facilitate incorporation of S protein into lentiviral pseudovirons, the last 19 amino acids containing an endoplasmic reticulum (ER)-retention signal from the cytoplasmic tail of the S protein was removed (Fig. 1a)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T45","span":{"begin":0,"end":373},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To enhance expression of the S protein of SARS-CoV-2 in mammalian cells, a codon-optimized cDNA encoding the S protein and 3xFLAG tag was synthesized, and to facilitate incorporation of S protein into lentiviral pseudovirons, the last 19 amino acids containing an endoplasmic reticulum (ER)-retention signal from the cytoplasmic tail of the S protein was removed (Fig. 1a)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"279","span":{"begin":29,"end":30},"obj":"Gene"},{"id":"280","span":{"begin":42,"end":52},"obj":"Species"},{"id":"281","span":{"begin":56,"end":65},"obj":"Species"}],"attributes":[{"id":"A279","pred":"tao:has_database_id","subj":"279","obj":"Gene:43740568"},{"id":"A280","pred":"tao:has_database_id","subj":"280","obj":"Tax:2697049"},{"id":"A281","pred":"tao:has_database_id","subj":"281","obj":"Tax:9606"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"To enhance expression of the S protein of SARS-CoV-2 in mammalian cells, a codon-optimized cDNA encoding the S protein and 3xFLAG tag was synthesized, and to facilitate incorporation of S protein into lentiviral pseudovirons, the last 19 amino acids containing an endoplasmic reticulum (ER)-retention signal from the cytoplasmic tail of the S protein was removed (Fig. 1a)."}