PMC:7100515 / 45266-45811 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T403","span":{"begin":220,"end":228},"obj":"Body_part"},{"id":"T404","span":{"begin":262,"end":267},"obj":"Body_part"},{"id":"T405","span":{"begin":355,"end":360},"obj":"Body_part"}],"attributes":[{"id":"A403","pred":"fma_id","subj":"T403","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A404","pred":"fma_id","subj":"T404","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A405","pred":"fma_id","subj":"T405","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Pseudovirus neutralization assay\nSARS-CoV S, SARS-CoV-2 S, and VSV-G pseudovirions were pre-incubated with serially diluted either polyclonal rabbit anti-SARS S1 antibodies T62 or patient sera for 1 h on ice, then virus-antibody mixture was added onto 293/hACE2 cells in a 96-well plate. After 6 h incubation, the inoculum was replaced with fresh medium. Cells were lysed 40 h later and pseudovirus transduction was measured as previously described. Prior to experiments, patient sera were incubated at 56 °C for 30 min to inactivate complement."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T371","span":{"begin":33,"end":41},"obj":"Disease"},{"id":"T372","span":{"begin":45,"end":53},"obj":"Disease"},{"id":"T373","span":{"begin":154,"end":158},"obj":"Disease"}],"attributes":[{"id":"A371","pred":"mondo_id","subj":"T371","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A372","pred":"mondo_id","subj":"T372","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A373","pred":"mondo_id","subj":"T373","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Pseudovirus neutralization assay\nSARS-CoV S, SARS-CoV-2 S, and VSV-G pseudovirions were pre-incubated with serially diluted either polyclonal rabbit anti-SARS S1 antibodies T62 or patient sera for 1 h on ice, then virus-antibody mixture was added onto 293/hACE2 cells in a 96-well plate. After 6 h incubation, the inoculum was replaced with fresh medium. Cells were lysed 40 h later and pseudovirus transduction was measured as previously described. Prior to experiments, patient sera were incubated at 56 °C for 30 min to inactivate complement."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1001","span":{"begin":159,"end":161},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T1002","span":{"begin":214,"end":219},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T1003","span":{"begin":252,"end":255},"obj":"http://purl.obolibrary.org/obo/CLO_0001230"},{"id":"T1004","span":{"begin":252,"end":255},"obj":"http://purl.obolibrary.org/obo/CLO_0037237"},{"id":"T1005","span":{"begin":252,"end":255},"obj":"http://purl.obolibrary.org/obo/CLO_0050903"},{"id":"T1006","span":{"begin":252,"end":255},"obj":"http://purl.obolibrary.org/obo/CLO_0054249"},{"id":"T1007","span":{"begin":252,"end":255},"obj":"http://purl.obolibrary.org/obo/CLO_0054250"},{"id":"T1008","span":{"begin":252,"end":255},"obj":"http://purl.obolibrary.org/obo/CLO_0054251"},{"id":"T1009","span":{"begin":252,"end":255},"obj":"http://purl.obolibrary.org/obo/CLO_0054252"},{"id":"T1010","span":{"begin":262,"end":267},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1011","span":{"begin":271,"end":272},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T1012","span":{"begin":355,"end":360},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Pseudovirus neutralization assay\nSARS-CoV S, SARS-CoV-2 S, and VSV-G pseudovirions were pre-incubated with serially diluted either polyclonal rabbit anti-SARS S1 antibodies T62 or patient sera for 1 h on ice, then virus-antibody mixture was added onto 293/hACE2 cells in a 96-well plate. After 6 h incubation, the inoculum was replaced with fresh medium. Cells were lysed 40 h later and pseudovirus transduction was measured as previously described. Prior to experiments, patient sera were incubated at 56 °C for 30 min to inactivate complement."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T402","span":{"begin":229,"end":236},"obj":"Chemical"}],"attributes":[{"id":"A402","pred":"chebi_id","subj":"T402","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"}],"text":"Pseudovirus neutralization assay\nSARS-CoV S, SARS-CoV-2 S, and VSV-G pseudovirions were pre-incubated with serially diluted either polyclonal rabbit anti-SARS S1 antibodies T62 or patient sera for 1 h on ice, then virus-antibody mixture was added onto 293/hACE2 cells in a 96-well plate. After 6 h incubation, the inoculum was replaced with fresh medium. Cells were lysed 40 h later and pseudovirus transduction was measured as previously described. Prior to experiments, patient sera were incubated at 56 °C for 30 min to inactivate complement."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T95","span":{"begin":399,"end":411},"obj":"http://purl.obolibrary.org/obo/GO_0009293"}],"text":"Pseudovirus neutralization assay\nSARS-CoV S, SARS-CoV-2 S, and VSV-G pseudovirions were pre-incubated with serially diluted either polyclonal rabbit anti-SARS S1 antibodies T62 or patient sera for 1 h on ice, then virus-antibody mixture was added onto 293/hACE2 cells in a 96-well plate. After 6 h incubation, the inoculum was replaced with fresh medium. Cells were lysed 40 h later and pseudovirus transduction was measured as previously described. Prior to experiments, patient sera were incubated at 56 °C for 30 min to inactivate complement."}

{"project":"LitCovid-sentences","denotations":[{"id":"T328","span":{"begin":0,"end":32},"obj":"Sentence"},{"id":"T329","span":{"begin":33,"end":287},"obj":"Sentence"},{"id":"T330","span":{"begin":288,"end":354},"obj":"Sentence"},{"id":"T331","span":{"begin":355,"end":449},"obj":"Sentence"},{"id":"T332","span":{"begin":450,"end":545},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Pseudovirus neutralization assay\nSARS-CoV S, SARS-CoV-2 S, and VSV-G pseudovirions were pre-incubated with serially diluted either polyclonal rabbit anti-SARS S1 antibodies T62 or patient sera for 1 h on ice, then virus-antibody mixture was added onto 293/hACE2 cells in a 96-well plate. After 6 h incubation, the inoculum was replaced with fresh medium. Cells were lysed 40 h later and pseudovirus transduction was measured as previously described. Prior to experiments, patient sera were incubated at 56 °C for 30 min to inactivate complement."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1747","span":{"begin":256,"end":261},"obj":"Gene"},{"id":"1748","span":{"begin":56,"end":57},"obj":"Gene"},{"id":"1749","span":{"begin":42,"end":43},"obj":"Gene"},{"id":"1750","span":{"begin":33,"end":41},"obj":"Species"},{"id":"1751","span":{"begin":45,"end":55},"obj":"Species"},{"id":"1752","span":{"begin":180,"end":187},"obj":"Species"},{"id":"1753","span":{"begin":472,"end":479},"obj":"Species"}],"attributes":[{"id":"A1747","pred":"tao:has_database_id","subj":"1747","obj":"Gene:59272"},{"id":"A1748","pred":"tao:has_database_id","subj":"1748","obj":"Gene:43740568"},{"id":"A1749","pred":"tao:has_database_id","subj":"1749","obj":"Gene:43740568"},{"id":"A1750","pred":"tao:has_database_id","subj":"1750","obj":"Tax:694009"},{"id":"A1751","pred":"tao:has_database_id","subj":"1751","obj":"Tax:2697049"},{"id":"A1752","pred":"tao:has_database_id","subj":"1752","obj":"Tax:9606"},{"id":"A1753","pred":"tao:has_database_id","subj":"1753","obj":"Tax:9606"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Pseudovirus neutralization assay\nSARS-CoV S, SARS-CoV-2 S, and VSV-G pseudovirions were pre-incubated with serially diluted either polyclonal rabbit anti-SARS S1 antibodies T62 or patient sera for 1 h on ice, then virus-antibody mixture was added onto 293/hACE2 cells in a 96-well plate. After 6 h incubation, the inoculum was replaced with fresh medium. Cells were lysed 40 h later and pseudovirus transduction was measured as previously described. Prior to experiments, patient sera were incubated at 56 °C for 30 min to inactivate complement."}