PMC:7100515 / 44852-45264 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T401","span":{"begin":185,"end":190},"obj":"Body_part"},{"id":"T402","span":{"begin":260,"end":265},"obj":"Body_part"}],"attributes":[{"id":"A401","pred":"fma_id","subj":"T401","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A402","pred":"fma_id","subj":"T402","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Briefly, lentiviruses pseudotyped with SARS-CoV-2 S, SARS-CoV S or VSV-G were pre-incubated with serially diluted soluble hACE2 for 1 h on ice. The mixture were then added on 293/hACE2 cells, followed by centrifugation inoculation for 1 h at room temperature. Cells were fed with fresh medium 6 h later and lysed at 48 h post inoculation. Pseudoviral transduction was measured according to luciferase activities."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T369","span":{"begin":39,"end":47},"obj":"Disease"},{"id":"T370","span":{"begin":53,"end":61},"obj":"Disease"}],"attributes":[{"id":"A369","pred":"mondo_id","subj":"T369","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A370","pred":"mondo_id","subj":"T370","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Briefly, lentiviruses pseudotyped with SARS-CoV-2 S, SARS-CoV S or VSV-G were pre-incubated with serially diluted soluble hACE2 for 1 h on ice. The mixture were then added on 293/hACE2 cells, followed by centrifugation inoculation for 1 h at room temperature. Cells were fed with fresh medium 6 h later and lysed at 48 h post inoculation. Pseudoviral transduction was measured according to luciferase activities."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T990","span":{"begin":175,"end":178},"obj":"http://purl.obolibrary.org/obo/CLO_0001230"},{"id":"T991","span":{"begin":175,"end":178},"obj":"http://purl.obolibrary.org/obo/CLO_0037237"},{"id":"T992","span":{"begin":175,"end":178},"obj":"http://purl.obolibrary.org/obo/CLO_0050903"},{"id":"T993","span":{"begin":175,"end":178},"obj":"http://purl.obolibrary.org/obo/CLO_0054249"},{"id":"T994","span":{"begin":175,"end":178},"obj":"http://purl.obolibrary.org/obo/CLO_0054250"},{"id":"T995","span":{"begin":175,"end":178},"obj":"http://purl.obolibrary.org/obo/CLO_0054251"},{"id":"T996","span":{"begin":175,"end":178},"obj":"http://purl.obolibrary.org/obo/CLO_0054252"},{"id":"T997","span":{"begin":185,"end":190},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T998","span":{"begin":260,"end":265},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T999","span":{"begin":316,"end":318},"obj":"http://purl.obolibrary.org/obo/CLO_0001382"},{"id":"T1000","span":{"begin":401,"end":411},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"}],"text":"Briefly, lentiviruses pseudotyped with SARS-CoV-2 S, SARS-CoV S or VSV-G were pre-incubated with serially diluted soluble hACE2 for 1 h on ice. The mixture were then added on 293/hACE2 cells, followed by centrifugation inoculation for 1 h at room temperature. Cells were fed with fresh medium 6 h later and lysed at 48 h post inoculation. Pseudoviral transduction was measured according to luciferase activities."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T401","span":{"begin":148,"end":155},"obj":"Chemical"}],"attributes":[{"id":"A401","pred":"chebi_id","subj":"T401","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"}],"text":"Briefly, lentiviruses pseudotyped with SARS-CoV-2 S, SARS-CoV S or VSV-G were pre-incubated with serially diluted soluble hACE2 for 1 h on ice. The mixture were then added on 293/hACE2 cells, followed by centrifugation inoculation for 1 h at room temperature. Cells were fed with fresh medium 6 h later and lysed at 48 h post inoculation. Pseudoviral transduction was measured according to luciferase activities."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T94","span":{"begin":351,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0009293"}],"text":"Briefly, lentiviruses pseudotyped with SARS-CoV-2 S, SARS-CoV S or VSV-G were pre-incubated with serially diluted soluble hACE2 for 1 h on ice. The mixture were then added on 293/hACE2 cells, followed by centrifugation inoculation for 1 h at room temperature. Cells were fed with fresh medium 6 h later and lysed at 48 h post inoculation. Pseudoviral transduction was measured according to luciferase activities."}

{"project":"LitCovid-sentences","denotations":[{"id":"T324","span":{"begin":0,"end":143},"obj":"Sentence"},{"id":"T325","span":{"begin":144,"end":259},"obj":"Sentence"},{"id":"T326","span":{"begin":260,"end":338},"obj":"Sentence"},{"id":"T327","span":{"begin":339,"end":412},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Briefly, lentiviruses pseudotyped with SARS-CoV-2 S, SARS-CoV S or VSV-G were pre-incubated with serially diluted soluble hACE2 for 1 h on ice. The mixture were then added on 293/hACE2 cells, followed by centrifugation inoculation for 1 h at room temperature. Cells were fed with fresh medium 6 h later and lysed at 48 h post inoculation. Pseudoviral transduction was measured according to luciferase activities."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1735","span":{"begin":122,"end":127},"obj":"Gene"},{"id":"1736","span":{"begin":179,"end":184},"obj":"Gene"},{"id":"1737","span":{"begin":50,"end":51},"obj":"Gene"},{"id":"1738","span":{"begin":39,"end":49},"obj":"Species"},{"id":"1739","span":{"begin":53,"end":61},"obj":"Species"}],"attributes":[{"id":"A1735","pred":"tao:has_database_id","subj":"1735","obj":"Gene:59272"},{"id":"A1736","pred":"tao:has_database_id","subj":"1736","obj":"Gene:59272"},{"id":"A1737","pred":"tao:has_database_id","subj":"1737","obj":"Gene:43740568"},{"id":"A1738","pred":"tao:has_database_id","subj":"1738","obj":"Tax:2697049"},{"id":"A1739","pred":"tao:has_database_id","subj":"1739","obj":"Tax:694009"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Briefly, lentiviruses pseudotyped with SARS-CoV-2 S, SARS-CoV S or VSV-G were pre-incubated with serially diluted soluble hACE2 for 1 h on ice. The mixture were then added on 293/hACE2 cells, followed by centrifugation inoculation for 1 h at room temperature. Cells were fed with fresh medium 6 h later and lysed at 48 h post inoculation. Pseudoviral transduction was measured according to luciferase activities."}