PMC:7100515 / 41889-42448 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T366","span":{"begin":30,"end":37},"obj":"Body_part"},{"id":"T367","span":{"begin":63,"end":68},"obj":"Body_part"},{"id":"T368","span":{"begin":163,"end":170},"obj":"Body_part"},{"id":"T369","span":{"begin":201,"end":206},"obj":"Body_part"},{"id":"T370","span":{"begin":265,"end":270},"obj":"Body_part"},{"id":"T371","span":{"begin":464,"end":467},"obj":"Body_part"},{"id":"T372","span":{"begin":516,"end":521},"obj":"Body_part"}],"attributes":[{"id":"A366","pred":"fma_id","subj":"T366","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A367","pred":"fma_id","subj":"T367","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A368","pred":"fma_id","subj":"T368","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A369","pred":"fma_id","subj":"T369","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A370","pred":"fma_id","subj":"T370","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A371","pred":"fma_id","subj":"T371","obj":"http://purl.org/sig/ont/fma/fma62872"},{"id":"A372","pred":"fma_id","subj":"T372","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Flow cytometric analysis of S protein expression\nBriefly, 293T cells were transfected with 2 μg of plasmids encoding either SARS-CoV-2 S, SARS-CoV S or MERS-CoV S protein using PEI. Forty hours later, cells were detached by using PBS with 1 mM EDTA. After washing, cells were incubated with polyclonal rabbit anti-SARS S1 antibodies T62 (1:200 dilution) (Sinobiological Inc., Beijing, China) for 1 h on ice, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200) (ZSGB-Bio LLC, Beijing, China) for 1 h. Cells were then analyzed by flow cytometry."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T359","span":{"begin":124,"end":132},"obj":"Disease"},{"id":"T360","span":{"begin":138,"end":146},"obj":"Disease"},{"id":"T361","span":{"begin":314,"end":318},"obj":"Disease"}],"attributes":[{"id":"A359","pred":"mondo_id","subj":"T359","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A360","pred":"mondo_id","subj":"T360","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A361","pred":"mondo_id","subj":"T361","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Flow cytometric analysis of S protein expression\nBriefly, 293T cells were transfected with 2 μg of plasmids encoding either SARS-CoV-2 S, SARS-CoV S or MERS-CoV S protein using PEI. Forty hours later, cells were detached by using PBS with 1 mM EDTA. After washing, cells were incubated with polyclonal rabbit anti-SARS S1 antibodies T62 (1:200 dilution) (Sinobiological Inc., Beijing, China) for 1 h on ice, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200) (ZSGB-Bio LLC, Beijing, China) for 1 h. Cells were then analyzed by flow cytometry."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T921","span":{"begin":58,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0050894"},{"id":"T922","span":{"begin":58,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0051650"},{"id":"T923","span":{"begin":58,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0052052"},{"id":"T924","span":{"begin":63,"end":68},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T925","span":{"begin":201,"end":206},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T926","span":{"begin":265,"end":270},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T927","span":{"begin":319,"end":321},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T928","span":{"begin":447,"end":451},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T929","span":{"begin":486,"end":489},"obj":"http://purl.obolibrary.org/obo/CLO_0050167"},{"id":"T930","span":{"begin":516,"end":521},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Flow cytometric analysis of S protein expression\nBriefly, 293T cells were transfected with 2 μg of plasmids encoding either SARS-CoV-2 S, SARS-CoV S or MERS-CoV S protein using PEI. Forty hours later, cells were detached by using PBS with 1 mM EDTA. After washing, cells were incubated with polyclonal rabbit anti-SARS S1 antibodies T62 (1:200 dilution) (Sinobiological Inc., Beijing, China) for 1 h on ice, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200) (ZSGB-Bio LLC, Beijing, China) for 1 h. Cells were then analyzed by flow cytometry."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T371","span":{"begin":30,"end":37},"obj":"Chemical"},{"id":"T372","span":{"begin":163,"end":170},"obj":"Chemical"},{"id":"T373","span":{"begin":177,"end":180},"obj":"Chemical"},{"id":"T375","span":{"begin":244,"end":248},"obj":"Chemical"},{"id":"T377","span":{"begin":420,"end":435},"obj":"Chemical"},{"id":"T378","span":{"begin":426,"end":431},"obj":"Chemical"}],"attributes":[{"id":"A371","pred":"chebi_id","subj":"T371","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A372","pred":"chebi_id","subj":"T372","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A373","pred":"chebi_id","subj":"T373","obj":"http://purl.obolibrary.org/obo/CHEBI_144613"},{"id":"A374","pred":"chebi_id","subj":"T373","obj":"http://purl.obolibrary.org/obo/CHEBI_53231"},{"id":"A375","pred":"chebi_id","subj":"T375","obj":"http://purl.obolibrary.org/obo/CHEBI_42191"},{"id":"A376","pred":"chebi_id","subj":"T375","obj":"http://purl.obolibrary.org/obo/CHEBI_64755"},{"id":"A377","pred":"chebi_id","subj":"T377","obj":"http://purl.obolibrary.org/obo/CHEBI_52661"},{"id":"A378","pred":"chebi_id","subj":"T378","obj":"http://purl.obolibrary.org/obo/CHEBI_24061"}],"text":"Flow cytometric analysis of S protein expression\nBriefly, 293T cells were transfected with 2 μg of plasmids encoding either SARS-CoV-2 S, SARS-CoV S or MERS-CoV S protein using PEI. Forty hours later, cells were detached by using PBS with 1 mM EDTA. After washing, cells were incubated with polyclonal rabbit anti-SARS S1 antibodies T62 (1:200 dilution) (Sinobiological Inc., Beijing, China) for 1 h on ice, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200) (ZSGB-Bio LLC, Beijing, China) for 1 h. Cells were then analyzed by flow cytometry."}

{"project":"LitCovid-sentences","denotations":[{"id":"T297","span":{"begin":0,"end":48},"obj":"Sentence"},{"id":"T298","span":{"begin":49,"end":181},"obj":"Sentence"},{"id":"T299","span":{"begin":182,"end":249},"obj":"Sentence"},{"id":"T300","span":{"begin":250,"end":515},"obj":"Sentence"},{"id":"T301","span":{"begin":516,"end":559},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Flow cytometric analysis of S protein expression\nBriefly, 293T cells were transfected with 2 μg of plasmids encoding either SARS-CoV-2 S, SARS-CoV S or MERS-CoV S protein using PEI. Forty hours later, cells were detached by using PBS with 1 mM EDTA. After washing, cells were incubated with polyclonal rabbit anti-SARS S1 antibodies T62 (1:200 dilution) (Sinobiological Inc., Beijing, China) for 1 h on ice, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200) (ZSGB-Bio LLC, Beijing, China) for 1 h. Cells were then analyzed by flow cytometry."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1666","span":{"begin":135,"end":136},"obj":"Gene"},{"id":"1667","span":{"begin":124,"end":134},"obj":"Species"},{"id":"1668","span":{"begin":138,"end":146},"obj":"Species"},{"id":"1669","span":{"begin":152,"end":160},"obj":"Species"},{"id":"1670","span":{"begin":230,"end":233},"obj":"Chemical"},{"id":"1671","span":{"begin":244,"end":248},"obj":"Chemical"},{"id":"1672","span":{"begin":420,"end":435},"obj":"Chemical"},{"id":"1673","span":{"begin":58,"end":62},"obj":"CellLine"}],"attributes":[{"id":"A1666","pred":"tao:has_database_id","subj":"1666","obj":"Gene:43740568"},{"id":"A1667","pred":"tao:has_database_id","subj":"1667","obj":"Tax:2697049"},{"id":"A1668","pred":"tao:has_database_id","subj":"1668","obj":"Tax:694009"},{"id":"A1669","pred":"tao:has_database_id","subj":"1669","obj":"Tax:1335626"},{"id":"A1670","pred":"tao:has_database_id","subj":"1670","obj":"MESH:D007854"},{"id":"A1671","pred":"tao:has_database_id","subj":"1671","obj":"MESH:D004492"},{"id":"A1673","pred":"tao:has_database_id","subj":"1673","obj":"CVCL:0063"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Flow cytometric analysis of S protein expression\nBriefly, 293T cells were transfected with 2 μg of plasmids encoding either SARS-CoV-2 S, SARS-CoV S or MERS-CoV S protein using PEI. Forty hours later, cells were detached by using PBS with 1 mM EDTA. After washing, cells were incubated with polyclonal rabbit anti-SARS S1 antibodies T62 (1:200 dilution) (Sinobiological Inc., Beijing, China) for 1 h on ice, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200) (ZSGB-Bio LLC, Beijing, China) for 1 h. Cells were then analyzed by flow cytometry."}