PMC:7100515 / 41284-41887 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7100515","sourcedb":"PMC","sourceid":"7100515","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7100515","text":"HEK 293/hACE2 cells were pretreated with either lysosomotropic agents (endosome acidification inhibitor NH4Cl and bafilomycin A; PIKfyve inhibitor apilimod and YM201636; calcium channel blocker tetrandrine) or cathepsin inhibitors (cathepsin L inhibitor SID26881509, cathepsin B inhibitor CA-074, and cathepsin inhibitor E64D) for 1 h at 37 °C, then spin-inoculated with pseudovirions in the presence of inhibitor at 1200 × g for 30 min. After overnight incubation, cells were fed with fresh medium without inhibitor. Cells were lysed at 48 h post inoculation and their luciferase activity was 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