PMC:7100515 / 41229-41887 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T362","span":{"begin":69,"end":74},"obj":"Body_part"},{"id":"T363","span":{"begin":126,"end":134},"obj":"Body_part"},{"id":"T364","span":{"begin":521,"end":526},"obj":"Body_part"},{"id":"T365","span":{"begin":573,"end":578},"obj":"Body_part"}],"attributes":[{"id":"A362","pred":"fma_id","subj":"T362","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A363","pred":"fma_id","subj":"T363","obj":"http://purl.org/sig/ont/fma/fma67180"},{"id":"A364","pred":"fma_id","subj":"T364","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A365","pred":"fma_id","subj":"T365","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Effects of agents and inhibitors on pseudovirion entry\nHEK 293/hACE2 cells were pretreated with either lysosomotropic agents (endosome acidification inhibitor NH4Cl and bafilomycin A; PIKfyve inhibitor apilimod and YM201636; calcium channel blocker tetrandrine) or cathepsin inhibitors (cathepsin L inhibitor SID26881509, cathepsin B inhibitor CA-074, and cathepsin inhibitor E64D) for 1 h at 37 °C, then spin-inoculated with pseudovirions in the presence of inhibitor at 1200 × g for 30 min. After overnight incubation, cells were fed with fresh medium without inhibitor. Cells were lysed at 48 h post inoculation and their luciferase activity was measured."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T907","span":{"begin":59,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0001230"},{"id":"T908","span":{"begin":59,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0037237"},{"id":"T909","span":{"begin":59,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0050903"},{"id":"T910","span":{"begin":59,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0054249"},{"id":"T911","span":{"begin":59,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0054250"},{"id":"T912","span":{"begin":59,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0054251"},{"id":"T913","span":{"begin":59,"end":62},"obj":"http://purl.obolibrary.org/obo/CLO_0054252"},{"id":"T914","span":{"begin":69,"end":74},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T915","span":{"begin":181,"end":182},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T916","span":{"begin":332,"end":333},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T917","span":{"begin":521,"end":526},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T918","span":{"begin":573,"end":578},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T919","span":{"begin":593,"end":595},"obj":"http://purl.obolibrary.org/obo/CLO_0001382"},{"id":"T920","span":{"begin":636,"end":644},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"}],"text":"Effects of agents and inhibitors on pseudovirion entry\nHEK 293/hACE2 cells were pretreated with either lysosomotropic agents (endosome acidification inhibitor NH4Cl and bafilomycin A; PIKfyve inhibitor apilimod and YM201636; calcium channel blocker tetrandrine) or cathepsin inhibitors (cathepsin L inhibitor SID26881509, cathepsin B inhibitor CA-074, and cathepsin inhibitor E64D) for 1 h at 37 °C, then spin-inoculated with pseudovirions in the presence of inhibitor at 1200 × g for 30 min. After overnight incubation, cells were fed with fresh medium without inhibitor. Cells were lysed at 48 h post inoculation and their luciferase activity was measured."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T356","span":{"begin":22,"end":32},"obj":"Chemical"},{"id":"T357","span":{"begin":149,"end":158},"obj":"Chemical"},{"id":"T358","span":{"begin":159,"end":164},"obj":"Chemical"},{"id":"T359","span":{"begin":192,"end":201},"obj":"Chemical"},{"id":"T360","span":{"begin":225,"end":248},"obj":"Chemical"},{"id":"T361","span":{"begin":225,"end":232},"obj":"Chemical"},{"id":"T363","span":{"begin":275,"end":285},"obj":"Chemical"},{"id":"T364","span":{"begin":287,"end":308},"obj":"Chemical"},{"id":"T365","span":{"begin":299,"end":308},"obj":"Chemical"},{"id":"T366","span":{"begin":322,"end":343},"obj":"Chemical"},{"id":"T367","span":{"begin":334,"end":343},"obj":"Chemical"},{"id":"T368","span":{"begin":366,"end":375},"obj":"Chemical"},{"id":"T369","span":{"begin":459,"end":468},"obj":"Chemical"},{"id":"T370","span":{"begin":562,"end":571},"obj":"Chemical"}],"attributes":[{"id":"A356","pred":"chebi_id","subj":"T356","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A357","pred":"chebi_id","subj":"T357","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A358","pred":"chebi_id","subj":"T358","obj":"http://purl.obolibrary.org/obo/CHEBI_31206"},{"id":"A359","pred":"chebi_id","subj":"T359","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A360","pred":"chebi_id","subj":"T360","obj":"http://purl.obolibrary.org/obo/CHEBI_38215"},{"id":"A361","pred":"chebi_id","subj":"T361","obj":"http://purl.obolibrary.org/obo/CHEBI_22984"},{"id":"A362","pred":"chebi_id","subj":"T361","obj":"http://purl.obolibrary.org/obo/CHEBI_29320"},{"id":"A363","pred":"chebi_id","subj":"T363","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A364","pred":"chebi_id","subj":"T364","obj":"http://purl.obolibrary.org/obo/CHEBI_70821"},{"id":"A365","pred":"chebi_id","subj":"T365","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A366","pred":"chebi_id","subj":"T366","obj":"http://purl.obolibrary.org/obo/CHEBI_64932"},{"id":"A367","pred":"chebi_id","subj":"T367","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A368","pred":"chebi_id","subj":"T368","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A369","pred":"chebi_id","subj":"T369","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A370","pred":"chebi_id","subj":"T370","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"}],"text":"Effects of agents and inhibitors on pseudovirion entry\nHEK 293/hACE2 cells were pretreated with either lysosomotropic agents (endosome acidification inhibitor NH4Cl and bafilomycin A; PIKfyve inhibitor apilimod and YM201636; calcium channel blocker tetrandrine) or cathepsin inhibitors (cathepsin L inhibitor SID26881509, cathepsin B inhibitor CA-074, and cathepsin inhibitor E64D) for 1 h at 37 °C, then spin-inoculated with pseudovirions in the presence of inhibitor at 1200 × g for 30 min. After overnight incubation, cells were fed with fresh medium without inhibitor. Cells were lysed at 48 h post inoculation and their luciferase activity was measured."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T80","span":{"begin":135,"end":148},"obj":"http://purl.obolibrary.org/obo/GO_0045851"}],"text":"Effects of agents and inhibitors on pseudovirion entry\nHEK 293/hACE2 cells were pretreated with either lysosomotropic agents (endosome acidification inhibitor NH4Cl and bafilomycin A; PIKfyve inhibitor apilimod and YM201636; calcium channel blocker tetrandrine) or cathepsin inhibitors (cathepsin L inhibitor SID26881509, cathepsin B inhibitor CA-074, and cathepsin inhibitor E64D) for 1 h at 37 °C, then spin-inoculated with pseudovirions in the presence of inhibitor at 1200 × g for 30 min. After overnight incubation, cells were fed with fresh medium without inhibitor. Cells were lysed at 48 h post inoculation and their luciferase activity was measured."}

{"project":"LitCovid-sentences","denotations":[{"id":"T293","span":{"begin":0,"end":54},"obj":"Sentence"},{"id":"T294","span":{"begin":55,"end":492},"obj":"Sentence"},{"id":"T295","span":{"begin":493,"end":572},"obj":"Sentence"},{"id":"T296","span":{"begin":573,"end":658},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Effects of agents and inhibitors on pseudovirion entry\nHEK 293/hACE2 cells were pretreated with either lysosomotropic agents (endosome acidification inhibitor NH4Cl and bafilomycin A; PIKfyve inhibitor apilimod and YM201636; calcium channel blocker tetrandrine) or cathepsin inhibitors (cathepsin L inhibitor SID26881509, cathepsin B inhibitor CA-074, and cathepsin inhibitor E64D) for 1 h at 37 °C, then spin-inoculated with pseudovirions in the presence of inhibitor at 1200 × g for 30 min. After overnight incubation, cells were fed with fresh medium without inhibitor. Cells were lysed at 48 h post inoculation and their luciferase activity was measured."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1654","span":{"begin":63,"end":68},"obj":"Gene"},{"id":"1655","span":{"begin":287,"end":298},"obj":"Gene"},{"id":"1656","span":{"begin":322,"end":333},"obj":"Gene"},{"id":"1657","span":{"begin":55,"end":62},"obj":"CellLine"}],"attributes":[{"id":"A1654","pred":"tao:has_database_id","subj":"1654","obj":"Gene:59272"},{"id":"A1655","pred":"tao:has_database_id","subj":"1655","obj":"Gene:1514"},{"id":"A1656","pred":"tao:has_database_id","subj":"1656","obj":"Gene:1508"},{"id":"A1657","pred":"tao:has_database_id","subj":"1657","obj":"CVCL:0045"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Effects of agents and inhibitors on pseudovirion entry\nHEK 293/hACE2 cells were pretreated with either lysosomotropic agents (endosome acidification inhibitor NH4Cl and bafilomycin A; PIKfyve inhibitor apilimod and YM201636; calcium channel blocker tetrandrine) or cathepsin inhibitors (cathepsin L inhibitor SID26881509, cathepsin B inhibitor CA-074, and cathepsin inhibitor E64D) for 1 h at 37 °C, then spin-inoculated with pseudovirions in the presence of inhibitor at 1200 × g for 30 min. After overnight incubation, cells were fed with fresh medium without inhibitor. Cells were lysed at 48 h post inoculation and their luciferase activity was measured."}