PMC:7100515 / 4070-5155 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T16","span":{"begin":37,"end":44},"obj":"Body_part"},{"id":"T17","span":{"begin":103,"end":110},"obj":"Body_part"},{"id":"T18","span":{"begin":340,"end":347},"obj":"Body_part"},{"id":"T19","span":{"begin":351,"end":356},"obj":"Body_part"},{"id":"T20","span":{"begin":392,"end":397},"obj":"Body_part"},{"id":"T21","span":{"begin":461,"end":469},"obj":"Body_part"},{"id":"T22","span":{"begin":504,"end":511},"obj":"Body_part"},{"id":"T23","span":{"begin":512,"end":520},"obj":"Body_part"},{"id":"T24","span":{"begin":607,"end":615},"obj":"Body_part"},{"id":"T25","span":{"begin":653,"end":661},"obj":"Body_part"},{"id":"T26","span":{"begin":687,"end":694},"obj":"Body_part"},{"id":"T27","span":{"begin":814,"end":821},"obj":"Body_part"},{"id":"T28","span":{"begin":822,"end":830},"obj":"Body_part"},{"id":"T29","span":{"begin":903,"end":906},"obj":"Body_part"},{"id":"T30","span":{"begin":935,"end":942},"obj":"Body_part"},{"id":"T31","span":{"begin":969,"end":976},"obj":"Body_part"}],"attributes":[{"id":"A16","pred":"fma_id","subj":"T16","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A17","pred":"fma_id","subj":"T17","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A18","pred":"fma_id","subj":"T18","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A19","pred":"fma_id","subj":"T19","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A20","pred":"fma_id","subj":"T20","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A21","pred":"fma_id","subj":"T21","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A22","pred":"fma_id","subj":"T22","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A23","pred":"fma_id","subj":"T23","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A24","pred":"fma_id","subj":"T24","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A25","pred":"fma_id","subj":"T25","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A26","pred":"fma_id","subj":"T26","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A27","pred":"fma_id","subj":"T27","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A28","pred":"fma_id","subj":"T28","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A29","pred":"fma_id","subj":"T29","obj":"http://purl.org/sig/ont/fma/fma63011"},{"id":"A30","pred":"fma_id","subj":"T30","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A31","pred":"fma_id","subj":"T31","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"Fig. 1 Incorporation of SARS-CoV-2 S protein into pseudovirions.\na Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b–f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g–j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T42","span":{"begin":24,"end":32},"obj":"Disease"},{"id":"T43","span":{"begin":90,"end":98},"obj":"Disease"},{"id":"T44","span":{"begin":215,"end":218},"obj":"Disease"},{"id":"T46","span":{"begin":551,"end":555},"obj":"Disease"},{"id":"T47","span":{"begin":599,"end":603},"obj":"Disease"},{"id":"T48","span":{"begin":861,"end":865},"obj":"Disease"}],"attributes":[{"id":"A42","pred":"mondo_id","subj":"T42","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A43","pred":"mondo_id","subj":"T43","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A44","pred":"mondo_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A45","pred":"mondo_id","subj":"T44","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"},{"id":"A46","pred":"mondo_id","subj":"T46","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A47","pred":"mondo_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A48","pred":"mondo_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Fig. 1 Incorporation of SARS-CoV-2 S protein into pseudovirions.\na Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b–f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g–j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T45","span":{"begin":65,"end":66},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T46","span":{"begin":116,"end":117},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T47","span":{"begin":130,"end":132},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T48","span":{"begin":160,"end":162},"obj":"http://purl.obolibrary.org/obo/CLO_0008922"},{"id":"T49","span":{"begin":160,"end":162},"obj":"http://purl.obolibrary.org/obo/CLO_0050052"},{"id":"T50","span":{"begin":164,"end":172},"obj":"http://purl.obolibrary.org/obo/UBERON_0000158"},{"id":"T51","span":{"begin":261,"end":268},"obj":"http://purl.obolibrary.org/obo/PR_000018263"},{"id":"T52","span":{"begin":316,"end":317},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T53","span":{"begin":351,"end":356},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T54","span":{"begin":387,"end":391},"obj":"http://purl.obolibrary.org/obo/CLO_0050894"},{"id":"T55","span":{"begin":387,"end":391},"obj":"http://purl.obolibrary.org/obo/CLO_0051650"},{"id":"T56","span":{"begin":387,"end":391},"obj":"http://purl.obolibrary.org/obo/CLO_0052052"},{"id":"T57","span":{"begin":392,"end":397},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T58","span":{"begin":429,"end":430},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T59","span":{"begin":431,"end":436},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"},{"id":"T60","span":{"begin":484,"end":488},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T61","span":{"begin":556,"end":558},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T62","span":{"begin":577,"end":582},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"},{"id":"T63","span":{"begin":604,"end":606},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T64","span":{"begin":619,"end":624},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"},{"id":"T65","span":{"begin":650,"end":652},"obj":"http://purl.obolibrary.org/obo/CLO_0008922"},{"id":"T66","span":{"begin":650,"end":652},"obj":"http://purl.obolibrary.org/obo/CLO_0050052"},{"id":"T67","span":{"begin":746,"end":747},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T68","span":{"begin":794,"end":798},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T69","span":{"begin":866,"end":868},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T70","span":{"begin":1066,"end":1067},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Fig. 1 Incorporation of SARS-CoV-2 S protein into pseudovirions.\na Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b–f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g–j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T31","span":{"begin":37,"end":44},"obj":"Chemical"},{"id":"T32","span":{"begin":103,"end":110},"obj":"Chemical"},{"id":"T33","span":{"begin":160,"end":162},"obj":"Chemical"},{"id":"T34","span":{"begin":189,"end":191},"obj":"Chemical"},{"id":"T36","span":{"begin":261,"end":268},"obj":"Chemical"},{"id":"T37","span":{"begin":340,"end":347},"obj":"Chemical"},{"id":"T38","span":{"begin":458,"end":460},"obj":"Chemical"},{"id":"T40","span":{"begin":504,"end":511},"obj":"Chemical"},{"id":"T41","span":{"begin":650,"end":652},"obj":"Chemical"},{"id":"T42","span":{"begin":687,"end":694},"obj":"Chemical"},{"id":"T43","span":{"begin":777,"end":779},"obj":"Chemical"},{"id":"T45","span":{"begin":814,"end":821},"obj":"Chemical"},{"id":"T46","span":{"begin":935,"end":942},"obj":"Chemical"},{"id":"T47","span":{"begin":969,"end":976},"obj":"Chemical"}],"attributes":[{"id":"A31","pred":"chebi_id","subj":"T31","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A32","pred":"chebi_id","subj":"T32","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A33","pred":"chebi_id","subj":"T33","obj":"http://purl.obolibrary.org/obo/CHEBI_29387"},{"id":"A34","pred":"chebi_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/CHEBI_55460"},{"id":"A35","pred":"chebi_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/CHEBI_74861"},{"id":"A36","pred":"chebi_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/CHEBI_16670"},{"id":"A37","pred":"chebi_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A38","pred":"chebi_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/CHEBI_34827"},{"id":"A39","pred":"chebi_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/CHEBI_51112"},{"id":"A40","pred":"chebi_id","subj":"T40","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A41","pred":"chebi_id","subj":"T41","obj":"http://purl.obolibrary.org/obo/CHEBI_29387"},{"id":"A42","pred":"chebi_id","subj":"T42","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A43","pred":"chebi_id","subj":"T43","obj":"http://purl.obolibrary.org/obo/CHEBI_34827"},{"id":"A44","pred":"chebi_id","subj":"T43","obj":"http://purl.obolibrary.org/obo/CHEBI_51112"},{"id":"A45","pred":"chebi_id","subj":"T45","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A46","pred":"chebi_id","subj":"T46","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A47","pred":"chebi_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"Fig. 1 Incorporation of SARS-CoV-2 S protein into pseudovirions.\na Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b–f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g–j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T6","span":{"begin":164,"end":179},"obj":"http://purl.obolibrary.org/obo/GO_0061025"}],"text":"Fig. 1 Incorporation of SARS-CoV-2 S protein into pseudovirions.\na Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b–f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g–j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file."}

{"project":"LitCovid-sentences","denotations":[{"id":"T30","span":{"begin":0,"end":64},"obj":"Sentence"},{"id":"T31","span":{"begin":65,"end":129},"obj":"Sentence"},{"id":"T32","span":{"begin":130,"end":380},"obj":"Sentence"},{"id":"T33","span":{"begin":381,"end":991},"obj":"Sentence"},{"id":"T34","span":{"begin":992,"end":1037},"obj":"Sentence"},{"id":"T35","span":{"begin":1038,"end":1085},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Fig. 1 Incorporation of SARS-CoV-2 S protein into pseudovirions.\na Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b–f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g–j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file."}

{"project":"LitCovid-PubTator","denotations":[{"id":"135","span":{"begin":24,"end":34},"obj":"Species"},{"id":"152","span":{"begin":732,"end":735},"obj":"Gene"},{"id":"153","span":{"begin":903,"end":906},"obj":"Gene"},{"id":"154","span":{"begin":907,"end":910},"obj":"Gene"},{"id":"155","span":{"begin":101,"end":102},"obj":"Gene"},{"id":"156","span":{"begin":728,"end":731},"obj":"Gene"},{"id":"157","span":{"begin":90,"end":100},"obj":"Species"},{"id":"158","span":{"begin":333,"end":337},"obj":"Species"},{"id":"159","span":{"begin":680,"end":684},"obj":"Species"},{"id":"160","span":{"begin":431,"end":436},"obj":"Species"},{"id":"161","span":{"begin":494,"end":497},"obj":"Species"},{"id":"162","span":{"begin":577,"end":582},"obj":"Species"},{"id":"163","span":{"begin":619,"end":624},"obj":"Species"},{"id":"164","span":{"begin":641,"end":649},"obj":"Species"},{"id":"165","span":{"begin":804,"end":807},"obj":"Species"},{"id":"166","span":{"begin":270,"end":274},"obj":"Disease"},{"id":"167","span":{"begin":387,"end":391},"obj":"CellLine"}],"attributes":[{"id":"A135","pred":"tao:has_database_id","subj":"135","obj":"Tax:2697049"},{"id":"A152","pred":"tao:has_database_id","subj":"152","obj":"Gene:140767"},{"id":"A153","pred":"tao:has_database_id","subj":"153","obj":"Gene:155030"},{"id":"A154","pred":"tao:has_database_id","subj":"154","obj":"Gene:140767"},{"id":"A155","pred":"tao:has_database_id","subj":"155","obj":"Gene:43740568"},{"id":"A156","pred":"tao:has_database_id","subj":"156","obj":"Gene:155030"},{"id":"A157","pred":"tao:has_database_id","subj":"157","obj":"Tax:2697049"},{"id":"A158","pred":"tao:has_database_id","subj":"158","obj":"Tax:11118"},{"id":"A159","pred":"tao:has_database_id","subj":"159","obj":"Tax:11118"},{"id":"A160","pred":"tao:has_database_id","subj":"160","obj":"Tax:10090"},{"id":"A161","pred":"tao:has_database_id","subj":"161","obj":"Tax:11138"},{"id":"A162","pred":"tao:has_database_id","subj":"162","obj":"Tax:10090"},{"id":"A163","pred":"tao:has_database_id","subj":"163","obj":"Tax:10090"},{"id":"A164","pred":"tao:has_database_id","subj":"164","obj":"Tax:1335626"},{"id":"A165","pred":"tao:has_database_id","subj":"165","obj":"Tax:11138"},{"id":"A166","pred":"tao:has_database_id","subj":"166","obj":"MESH:D001919"},{"id":"A167","pred":"tao:has_database_id","subj":"167","obj":"CVCL:0063"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Fig. 1 Incorporation of SARS-CoV-2 S protein into pseudovirions.\na Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b–f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g–j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file."}