PMC:7100515 / 40477-40707 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T355","span":{"begin":20,"end":24},"obj":"Body_part"}],"attributes":[{"id":"A355","pred":"fma_id","subj":"T355","obj":"http://purl.org/sig/ont/fma/fma62100"}],"text":"After blocked by 5% milk, the membranes were blotted with primary antibodies, followed by incubated with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000) and visualized with Chemiluminescent Reagent (Bio-Rad)."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T23","span":{"begin":20,"end":24},"obj":"Body_part"}],"attributes":[{"id":"A23","pred":"uberon_id","subj":"T23","obj":"http://purl.obolibrary.org/obo/UBERON_0001913"}],"text":"After blocked by 5% milk, the membranes were blotted with primary antibodies, followed by incubated with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000) and visualized with Chemiluminescent Reagent (Bio-Rad)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T898","span":{"begin":30,"end":39},"obj":"http://purl.obolibrary.org/obo/UBERON_0000158"}],"text":"After blocked by 5% milk, the membranes were blotted with primary antibodies, followed by incubated with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000) and visualized with Chemiluminescent Reagent (Bio-Rad)."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T79","span":{"begin":105,"end":133},"obj":"http://purl.obolibrary.org/obo/GO_0004601"}],"text":"After blocked by 5% milk, the membranes were blotted with primary antibodies, followed by incubated with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000) and visualized with Chemiluminescent Reagent (Bio-Rad)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T291","span":{"begin":0,"end":230},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"After blocked by 5% milk, the membranes were blotted with primary antibodies, followed by incubated with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000) and visualized with Chemiluminescent Reagent (Bio-Rad)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1633","span":{"begin":105,"end":116},"obj":"Species"}],"attributes":[{"id":"A1633","pred":"tao:has_database_id","subj":"1633","obj":"Tax:3704"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"After blocked by 5% milk, the membranes were blotted with primary antibodies, followed by incubated with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000) and visualized with Chemiluminescent Reagent (Bio-Rad)."}