PMC:7100515 / 39773-41227
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T351 | 10-22 | Body_part | denotes | glycoprotein | http://purl.org/sig/ont/fma/fma62925 |
| T352 | 40-45 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T353 | 113-118 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T354 | 160-172 | Body_part | denotes | glycoprotein | http://purl.org/sig/ont/fma/fma62925 |
| T355 | 724-728 | Body_part | denotes | milk | http://purl.org/sig/ont/fma/fma62100 |
| T356 | 941-949 | Body_part | denotes | proteins | http://purl.org/sig/ont/fma/fma67257 |
| T357 | 997-1005 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T358 | 1031-1039 | Body_part | denotes | proteins | http://purl.org/sig/ont/fma/fma67257 |
| T359 | 1186-1194 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T360 | 1273-1281 | Body_part | denotes | proteins | http://purl.org/sig/ont/fma/fma67257 |
| T361 | 1394-1402 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
LitCovid-PD-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T23 | 724-728 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T351 | 26-34 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T352 | 176-184 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T353 | 188-196 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T354 | 321-327 | Disease | denotes | SDS, 1 | http://purl.obolibrary.org/obo/MONDO_0044204 |
| T355 | 1020-1028 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T356 | 1081-1085 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T357 | 1178-1182 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T358 | 1260-1268 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T889 | 40-45 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T890 | 113-118 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T891 | 462-463 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T892 | 486-490 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
| T893 | 499-503 | http://purl.obolibrary.org/obo/CLO_0001564 | denotes | a 20 |
| T894 | 499-503 | http://purl.obolibrary.org/obo/CLO_0050194 | denotes | a 20 |
| T895 | 526-531 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
| T896 | 633-637 | http://purl.obolibrary.org/obo/CLO_0001550 | denotes | a 10 |
| T897 | 693-702 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membranes |
| T898 | 734-743 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membranes |
| T899 | 981-985 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
| T900 | 1086-1088 | http://purl.obolibrary.org/obo/CLO_0050050 | denotes | S1 |
| T901 | 1153-1158 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T902 | 1183-1185 | http://purl.obolibrary.org/obo/CLO_0050050 | denotes | S1 |
| T903 | 1302-1307 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T904 | 1420-1422 | http://purl.obolibrary.org/obo/CLO_0009141 | denotes | St |
| T905 | 1420-1422 | http://purl.obolibrary.org/obo/CLO_0050980 | denotes | St |
| T906 | 1431-1433 | http://purl.obolibrary.org/obo/CLO_0007815 | denotes | MO |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T334 | 10-22 | Chemical | denotes | glycoprotein | http://purl.obolibrary.org/obo/CHEBI_17089 |
| T335 | 160-172 | Chemical | denotes | glycoprotein | http://purl.obolibrary.org/obo/CHEBI_17089 |
| T336 | 261-267 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T337 | 275-279 | Chemical | denotes | Tris | http://purl.obolibrary.org/obo/CHEBI_9754 |
| T338 | 280-283 | Chemical | denotes | HCl | http://purl.obolibrary.org/obo/CHEBI_17883 |
| T339 | 299-303 | Chemical | denotes | NaCl | http://purl.obolibrary.org/obo/CHEBI_26710 |
| T340 | 310-314 | Chemical | denotes | EDTA | http://purl.obolibrary.org/obo/CHEBI_42191|http://purl.obolibrary.org/obo/CHEBI_64755 |
| T342 | 321-324 | Chemical | denotes | SDS | http://purl.obolibrary.org/obo/CHEBI_8984 |
| T343 | 329-333 | Chemical | denotes | NP40 | http://purl.obolibrary.org/obo/CHEBI_63016 |
| T344 | 337-355 | Chemical | denotes | protease inhibitor | http://purl.obolibrary.org/obo/CHEBI_37670|http://purl.obolibrary.org/obo/CHEBI_60258 |
| T346 | 346-355 | Chemical | denotes | inhibitor | http://purl.obolibrary.org/obo/CHEBI_35222 |
| T347 | 505-512 | Chemical | denotes | sucrose | http://purl.obolibrary.org/obo/CHEBI_17992 |
| T348 | 573-579 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T349 | 639-642 | Chemical | denotes | SDS | http://purl.obolibrary.org/obo/CHEBI_8984 |
| T350 | 671-685 | Chemical | denotes | nitrocellulose | http://purl.obolibrary.org/obo/CHEBI_53325 |
| T351 | 941-949 | Chemical | denotes | proteins | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T352 | 1031-1039 | Chemical | denotes | proteins | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T353 | 1273-1281 | Chemical | denotes | proteins | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T354 | 1391-1393 | Chemical | denotes | M2 | http://purl.obolibrary.org/obo/CHEBI_34827|http://purl.obolibrary.org/obo/CHEBI_51112 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T79 | 809-837 | http://purl.obolibrary.org/obo/GO_0004601 | denotes | horseradish peroxidase (HRP) |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T287 | 0-103 | Sentence | denotes | The spike glycoprotein of SARS-CoV-2 in cells and on pseudovirions were detected by using western blot. |
| T288 | 104-366 | Sentence | denotes | Briefly, cells transfected with plasmids encoding spike glycoprotein of SARS-CoV-2, SARS-CoV, MERS-CoV, and MHV were lysed at 40 h post transfection by RIPA buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% SDS, 1% NP40, 1×protease inhibitor cocktail). |
| T289 | 367-580 | Sentence | denotes | To pellet down pseudovirions, the viral supernatants were centrifuged at 25,000 rpm for 2 h in a Beckman SW41 rotor at 4 °C through a 20% sucrose cushion, and virus pellets were resuspended into 30 μl RIPA buffer. |
| T290 | 581-703 | Sentence | denotes | The samples were boiled for 10 min and separated in a 10% SDS-PAGE gel and transferred to nitrocellulose filter membranes. |
| T291 | 704-934 | Sentence | denotes | After blocked by 5% milk, the membranes were blotted with primary antibodies, followed by incubated with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000) and visualized with Chemiluminescent Reagent (Bio-Rad). |
| T292 | 935-1454 | Sentence | denotes | MHV S proteins were detected using polyclonal goat anti-MHV S antibody AO4 (1:2000); SARS-CoV S proteins were blotted with either polyclonal anti-SARS S1 antibodies T62 (1:2000) (Sinobiological Inc, Beijing, China) or mouse monoclonal against SARS S1 antibody MM02 (1:1000) (Sinobiological Inc, Beijing, China), MERS-CoV and SARS-CoV-2 S proteins were detected using mouse monoclonal anti-MERS S (1:1000) (Sinobiological Inc, Beijing, China) and anti-FLAG M2 antibody (1:1000) (Sigma, St. Louis, MO, USA), respectively. |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 1628 | 1271-1272 | Gene | denotes | S | Gene:43740568 |
| 1629 | 26-36 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 1630 | 176-186 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 1631 | 188-196 | Species | denotes | SARS-CoV | Tax:694009 |
| 1632 | 198-206 | Species | denotes | MERS-CoV | Tax:1335626 |
| 1633 | 809-820 | Species | denotes | horseradish | Tax:3704 |
| 1634 | 935-940 | Species | denotes | MHV S | Tax:11145 |
| 1635 | 1020-1028 | Species | denotes | SARS-CoV | Tax:694009 |
| 1636 | 1247-1255 | Species | denotes | MERS-CoV | Tax:1335626 |
| 1637 | 1260-1270 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 1638 | 212-215 | Species | denotes | MHV | Tax:11138 |
| 1639 | 991-994 | Species | denotes | MHV | Tax:11138 |
| 1640 | 1153-1158 | Species | denotes | mouse | Tax:10090 |
| 1641 | 1302-1307 | Species | denotes | mouse | Tax:10090 |
| 1642 | 256-267 | Chemical | denotes | RIPA buffer | |
| 1643 | 275-279 | Chemical | denotes | Tris | |
| 1644 | 280-283 | Chemical | denotes | HCl | MESH:D006851 |
| 1645 | 299-303 | Chemical | denotes | NaCl | MESH:D012965 |
| 1646 | 310-314 | Chemical | denotes | EDTA | MESH:D004492 |
| 1647 | 321-324 | Chemical | denotes | SDS | MESH:D012967 |
| 1648 | 505-512 | Chemical | denotes | sucrose | MESH:D013395 |
| 1649 | 639-642 | Chemical | denotes | SDS | MESH:D012967 |