PMC:7100515 / 11267-12701 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T99","span":{"begin":54,"end":58},"obj":"Body_part"},{"id":"T100","span":{"begin":66,"end":71},"obj":"Body_part"},{"id":"T101","span":{"begin":329,"end":339},"obj":"Body_part"},{"id":"T102","span":{"begin":355,"end":360},"obj":"Body_part"},{"id":"T103","span":{"begin":424,"end":429},"obj":"Body_part"},{"id":"T104","span":{"begin":495,"end":500},"obj":"Body_part"},{"id":"T105","span":{"begin":722,"end":730},"obj":"Body_part"},{"id":"T106","span":{"begin":757,"end":762},"obj":"Body_part"},{"id":"T107","span":{"begin":812,"end":820},"obj":"Body_part"},{"id":"T108","span":{"begin":906,"end":914},"obj":"Body_part"},{"id":"T109","span":{"begin":916,"end":921},"obj":"Body_part"},{"id":"T110","span":{"begin":1198,"end":1203},"obj":"Body_part"},{"id":"T111","span":{"begin":1205,"end":1210},"obj":"Body_part"}],"attributes":[{"id":"A99","pred":"fma_id","subj":"T99","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A100","pred":"fma_id","subj":"T100","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A101","pred":"fma_id","subj":"T101","obj":"http://purl.org/sig/ont/fma/fma63877"},{"id":"A102","pred":"fma_id","subj":"T102","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A103","pred":"fma_id","subj":"T103","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A104","pred":"fma_id","subj":"T104","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A105","pred":"fma_id","subj":"T105","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A106","pred":"fma_id","subj":"T106","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A107","pred":"fma_id","subj":"T107","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A108","pred":"fma_id","subj":"T108","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A109","pred":"fma_id","subj":"T109","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A110","pred":"fma_id","subj":"T110","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A111","pred":"fma_id","subj":"T111","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"a, b Entry of SARS-CoV-2 S pseudovirions on indicated cell lines. Cells from human and animal origin were inoculated with SARS-CoV-2 S (red), SARS-CoV S (blue), or VSV-G (gray) pseudovirions. At 48 h post inoculation, transduction efficiency was measured according to luciferase activities. RS, Rhinolophus sinicus bat embryonic fibroblast; BHK/hAPN, BHK cells stably expressing hAPN, the hCoV-229E receptor; 293/hACE2, 293 cells stably expressing hACE2, the SARS-CoV receptor; HeLa/hDPP4, HeLa cells stably expressing hDPP4, the MERS-CoV receptor. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. c Binding of SARS-CoV S and SARS-CoV-2 S proteins to soluble hACE2. HEK293T cells transiently expressing SARS-CoV and SARS-CoV-2 S proteins were incubated with the soluble hACE2 on ice, followed by polyclonal goat anti-hACE2 antibody. Cells were analyzed by flow cytometry. The experiments were repeated at least three times. d Inhibition of SARS-CoV-2 S pseudovirion entry by soluble hACE2. SARS-CoV S, SARS-CoV-2 S, or VSV-G pseudovirions were pre-incubated with soluble hACE2, then mixture were added to 293/hACE2 cells. Cells were lysed 40 h later and pseudoviral transduction was measured. Experiments were done twice and one representative is shown. Error bars indicate SEM of technical triplicates. Source data are provided as a Source Data file."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T104","span":{"begin":14,"end":22},"obj":"Disease"},{"id":"T105","span":{"begin":122,"end":130},"obj":"Disease"},{"id":"T106","span":{"begin":142,"end":150},"obj":"Disease"},{"id":"T107","span":{"begin":291,"end":293},"obj":"Disease"},{"id":"T108","span":{"begin":459,"end":467},"obj":"Disease"},{"id":"T109","span":{"begin":694,"end":702},"obj":"Disease"},{"id":"T110","span":{"begin":709,"end":717},"obj":"Disease"},{"id":"T111","span":{"begin":786,"end":794},"obj":"Disease"},{"id":"T112","span":{"begin":799,"end":807},"obj":"Disease"},{"id":"T113","span":{"begin":1023,"end":1031},"obj":"Disease"},{"id":"T114","span":{"begin":1073,"end":1081},"obj":"Disease"},{"id":"T115","span":{"begin":1085,"end":1093},"obj":"Disease"}],"attributes":[{"id":"A104","pred":"mondo_id","subj":"T104","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A105","pred":"mondo_id","subj":"T105","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A106","pred":"mondo_id","subj":"T106","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A107","pred":"mondo_id","subj":"T107","obj":"http://purl.obolibrary.org/obo/MONDO_0010725"},{"id":"A108","pred":"mondo_id","subj":"T108","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A109","pred":"mondo_id","subj":"T109","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A110","pred":"mondo_id","subj":"T110","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A111","pred":"mondo_id","subj":"T111","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A112","pred":"mondo_id","subj":"T112","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A113","pred":"mondo_id","subj":"T113","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A114","pred":"mondo_id","subj":"T114","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A115","pred":"mondo_id","subj":"T115","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"a, b Entry of SARS-CoV-2 S pseudovirions on indicated cell lines. Cells from human and animal origin were inoculated with SARS-CoV-2 S (red), SARS-CoV S (blue), or VSV-G (gray) pseudovirions. At 48 h post inoculation, transduction efficiency was measured according to luciferase activities. RS, Rhinolophus sinicus bat embryonic fibroblast; BHK/hAPN, BHK cells stably expressing hAPN, the hCoV-229E receptor; 293/hACE2, 293 cells stably expressing hACE2, the SARS-CoV receptor; HeLa/hDPP4, HeLa cells stably expressing hDPP4, the MERS-CoV receptor. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. c Binding of SARS-CoV S and SARS-CoV-2 S proteins to soluble hACE2. HEK293T cells transiently expressing SARS-CoV and SARS-CoV-2 S proteins were incubated with the soluble hACE2 on ice, followed by polyclonal goat anti-hACE2 antibody. Cells were analyzed by flow cytometry. The experiments were repeated at least three times. d Inhibition of SARS-CoV-2 S pseudovirion entry by soluble hACE2. SARS-CoV S, SARS-CoV-2 S, or VSV-G pseudovirions were pre-incubated with soluble hACE2, then mixture were added to 293/hACE2 cells. Cells were lysed 40 h later and pseudoviral transduction was measured. Experiments were done twice and one representative is shown. Error bars indicate SEM of technical triplicates. Source data are provided as a Source Data file."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T197","span":{"begin":0,"end":1},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T198","span":{"begin":3,"end":4},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T199","span":{"begin":54,"end":64},"obj":"http://purl.obolibrary.org/obo/CLO_0000031"},{"id":"T200","span":{"begin":66,"end":71},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T201","span":{"begin":77,"end":82},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T202","span":{"begin":87,"end":93},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_33208"},{"id":"T203","span":{"begin":195,"end":197},"obj":"http://purl.obolibrary.org/obo/CLO_0001382"},{"id":"T204","span":{"begin":279,"end":289},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"},{"id":"T205","span":{"begin":291,"end":293},"obj":"http://purl.obolibrary.org/obo/CLO_0008882"},{"id":"T206","span":{"begin":315,"end":318},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9397"},{"id":"T207","span":{"begin":329,"end":339},"obj":"http://purl.obolibrary.org/obo/CL_0000057"},{"id":"T208","span":{"begin":355,"end":360},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T209","span":{"begin":409,"end":412},"obj":"http://purl.obolibrary.org/obo/CLO_0001230"},{"id":"T210","span":{"begin":409,"end":412},"obj":"http://purl.obolibrary.org/obo/CLO_0037237"},{"id":"T211","span":{"begin":409,"end":412},"obj":"http://purl.obolibrary.org/obo/CLO_0050903"},{"id":"T212","span":{"begin":409,"end":412},"obj":"http://purl.obolibrary.org/obo/CLO_0054249"},{"id":"T213","span":{"begin":409,"end":412},"obj":"http://purl.obolibrary.org/obo/CLO_0054250"},{"id":"T214","span":{"begin":409,"end":412},"obj":"http://purl.obolibrary.org/obo/CLO_0054251"},{"id":"T215","span":{"begin":409,"end":412},"obj":"http://purl.obolibrary.org/obo/CLO_0054252"},{"id":"T216","span":{"begin":420,"end":423},"obj":"http://purl.obolibrary.org/obo/CLO_0001230"},{"id":"T217","span":{"begin":420,"end":423},"obj":"http://purl.obolibrary.org/obo/CLO_0037237"},{"id":"T218","span":{"begin":420,"end":423},"obj":"http://purl.obolibrary.org/obo/CLO_0050903"},{"id":"T219","span":{"begin":420,"end":423},"obj":"http://purl.obolibrary.org/obo/CLO_0054249"},{"id":"T220","span":{"begin":420,"end":423},"obj":"http://purl.obolibrary.org/obo/CLO_0054250"},{"id":"T221","span":{"begin":420,"end":423},"obj":"http://purl.obolibrary.org/obo/CLO_0054251"},{"id":"T222","span":{"begin":420,"end":423},"obj":"http://purl.obolibrary.org/obo/CLO_0054252"},{"id":"T223","span":{"begin":424,"end":429},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T224","span":{"begin":478,"end":482},"obj":"http://purl.obolibrary.org/obo/CLO_0003684"},{"id":"T225","span":{"begin":478,"end":482},"obj":"http://purl.obolibrary.org/obo/CLO_0050910"},{"id":"T226","span":{"begin":490,"end":500},"obj":"http://purl.obolibrary.org/obo/CLO_0054283"},{"id":"T227","span":{"begin":490,"end":500},"obj":"http://purl.obolibrary.org/obo/CLO_0054285"},{"id":"T228","span":{"begin":490,"end":500},"obj":"http://purl.obolibrary.org/obo/CLO_0054286"},{"id":"T229","span":{"begin":490,"end":500},"obj":"http://purl.obolibrary.org/obo/CLO_0054287"},{"id":"T230","span":{"begin":490,"end":500},"obj":"http://purl.obolibrary.org/obo/CLO_0054288"},{"id":"T231","span":{"begin":490,"end":500},"obj":"http://purl.obolibrary.org/obo/CLO_0054289"},{"id":"T232","span":{"begin":490,"end":494},"obj":"http://purl.obolibrary.org/obo/CLO_0003684"},{"id":"T233","span":{"begin":490,"end":494},"obj":"http://purl.obolibrary.org/obo/CLO_0050910"},{"id":"T234","span":{"begin":757,"end":762},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T235","span":{"begin":890,"end":894},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T236","span":{"begin":916,"end":921},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T237","span":{"begin":1188,"end":1191},"obj":"http://purl.obolibrary.org/obo/CLO_0001230"},{"id":"T238","span":{"begin":1188,"end":1191},"obj":"http://purl.obolibrary.org/obo/CLO_0037237"},{"id":"T239","span":{"begin":1188,"end":1191},"obj":"http://purl.obolibrary.org/obo/CLO_0050903"},{"id":"T240","span":{"begin":1188,"end":1191},"obj":"http://purl.obolibrary.org/obo/CLO_0054249"},{"id":"T241","span":{"begin":1188,"end":1191},"obj":"http://purl.obolibrary.org/obo/CLO_0054250"},{"id":"T242","span":{"begin":1188,"end":1191},"obj":"http://purl.obolibrary.org/obo/CLO_0054251"},{"id":"T243","span":{"begin":1188,"end":1191},"obj":"http://purl.obolibrary.org/obo/CLO_0054252"},{"id":"T244","span":{"begin":1198,"end":1203},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T245","span":{"begin":1205,"end":1210},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T246","span":{"begin":1415,"end":1416},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"a, b Entry of SARS-CoV-2 S pseudovirions on indicated cell lines. Cells from human and animal origin were inoculated with SARS-CoV-2 S (red), SARS-CoV S (blue), or VSV-G (gray) pseudovirions. At 48 h post inoculation, transduction efficiency was measured according to luciferase activities. RS, Rhinolophus sinicus bat embryonic fibroblast; BHK/hAPN, BHK cells stably expressing hAPN, the hCoV-229E receptor; 293/hACE2, 293 cells stably expressing hACE2, the SARS-CoV receptor; HeLa/hDPP4, HeLa cells stably expressing hDPP4, the MERS-CoV receptor. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. c Binding of SARS-CoV S and SARS-CoV-2 S proteins to soluble hACE2. HEK293T cells transiently expressing SARS-CoV and SARS-CoV-2 S proteins were incubated with the soluble hACE2 on ice, followed by polyclonal goat anti-hACE2 antibody. Cells were analyzed by flow cytometry. The experiments were repeated at least three times. d Inhibition of SARS-CoV-2 S pseudovirion entry by soluble hACE2. SARS-CoV S, SARS-CoV-2 S, or VSV-G pseudovirions were pre-incubated with soluble hACE2, then mixture were added to 293/hACE2 cells. Cells were lysed 40 h later and pseudoviral transduction was measured. Experiments were done twice and one representative is shown. Error bars indicate SEM of technical triplicates. Source data are provided as a Source Data file."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T104","span":{"begin":291,"end":293},"obj":"Chemical"},{"id":"T105","span":{"begin":722,"end":730},"obj":"Chemical"},{"id":"T106","span":{"begin":812,"end":820},"obj":"Chemical"},{"id":"T107","span":{"begin":1166,"end":1173},"obj":"Chemical"}],"attributes":[{"id":"A104","pred":"chebi_id","subj":"T104","obj":"http://purl.obolibrary.org/obo/CHEBI_73819"},{"id":"A105","pred":"chebi_id","subj":"T105","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A106","pred":"chebi_id","subj":"T106","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A107","pred":"chebi_id","subj":"T107","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"}],"text":"a, b Entry of SARS-CoV-2 S pseudovirions on indicated cell lines. Cells from human and animal origin were inoculated with SARS-CoV-2 S (red), SARS-CoV S (blue), or VSV-G (gray) pseudovirions. At 48 h post inoculation, transduction efficiency was measured according to luciferase activities. RS, Rhinolophus sinicus bat embryonic fibroblast; BHK/hAPN, BHK cells stably expressing hAPN, the hCoV-229E receptor; 293/hACE2, 293 cells stably expressing hACE2, the SARS-CoV receptor; HeLa/hDPP4, HeLa cells stably expressing hDPP4, the MERS-CoV receptor. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. c Binding of SARS-CoV S and SARS-CoV-2 S proteins to soluble hACE2. HEK293T cells transiently expressing SARS-CoV and SARS-CoV-2 S proteins were incubated with the soluble hACE2 on ice, followed by polyclonal goat anti-hACE2 antibody. Cells were analyzed by flow cytometry. The experiments were repeated at least three times. d Inhibition of SARS-CoV-2 S pseudovirion entry by soluble hACE2. SARS-CoV S, SARS-CoV-2 S, or VSV-G pseudovirions were pre-incubated with soluble hACE2, then mixture were added to 293/hACE2 cells. Cells were lysed 40 h later and pseudoviral transduction was measured. Experiments were done twice and one representative is shown. Error bars indicate SEM of technical triplicates. Source data are provided as a Source Data file."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T11","span":{"begin":218,"end":230},"obj":"http://purl.obolibrary.org/obo/GO_0009293"},{"id":"T12","span":{"begin":1249,"end":1261},"obj":"http://purl.obolibrary.org/obo/GO_0009293"}],"text":"a, b Entry of SARS-CoV-2 S pseudovirions on indicated cell lines. Cells from human and animal origin were inoculated with SARS-CoV-2 S (red), SARS-CoV S (blue), or VSV-G (gray) pseudovirions. At 48 h post inoculation, transduction efficiency was measured according to luciferase activities. RS, Rhinolophus sinicus bat embryonic fibroblast; BHK/hAPN, BHK cells stably expressing hAPN, the hCoV-229E receptor; 293/hACE2, 293 cells stably expressing hACE2, the SARS-CoV receptor; HeLa/hDPP4, HeLa cells stably expressing hDPP4, the MERS-CoV receptor. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. c Binding of SARS-CoV S and SARS-CoV-2 S proteins to soluble hACE2. HEK293T cells transiently expressing SARS-CoV and SARS-CoV-2 S proteins were incubated with the soluble hACE2 on ice, followed by polyclonal goat anti-hACE2 antibody. Cells were analyzed by flow cytometry. The experiments were repeated at least three times. d Inhibition of SARS-CoV-2 S pseudovirion entry by soluble hACE2. SARS-CoV S, SARS-CoV-2 S, or VSV-G pseudovirions were pre-incubated with soluble hACE2, then mixture were added to 293/hACE2 cells. Cells were lysed 40 h later and pseudoviral transduction was measured. Experiments were done twice and one representative is shown. Error bars indicate SEM of technical triplicates. Source data are provided as a Source Data file."}

{"project":"LitCovid-sentences","denotations":[{"id":"T77","span":{"begin":0,"end":65},"obj":"Sentence"},{"id":"T78","span":{"begin":66,"end":191},"obj":"Sentence"},{"id":"T79","span":{"begin":192,"end":290},"obj":"Sentence"},{"id":"T80","span":{"begin":291,"end":548},"obj":"Sentence"},{"id":"T81","span":{"begin":549,"end":620},"obj":"Sentence"},{"id":"T82","span":{"begin":621,"end":748},"obj":"Sentence"},{"id":"T83","span":{"begin":749,"end":915},"obj":"Sentence"},{"id":"T84","span":{"begin":916,"end":954},"obj":"Sentence"},{"id":"T85","span":{"begin":955,"end":1072},"obj":"Sentence"},{"id":"T86","span":{"begin":1073,"end":1204},"obj":"Sentence"},{"id":"T87","span":{"begin":1205,"end":1275},"obj":"Sentence"},{"id":"T88","span":{"begin":1276,"end":1336},"obj":"Sentence"},{"id":"T89","span":{"begin":1337,"end":1386},"obj":"Sentence"},{"id":"T90","span":{"begin":1387,"end":1434},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"a, b Entry of SARS-CoV-2 S pseudovirions on indicated cell lines. Cells from human and animal origin were inoculated with SARS-CoV-2 S (red), SARS-CoV S (blue), or VSV-G (gray) pseudovirions. At 48 h post inoculation, transduction efficiency was measured according to luciferase activities. RS, Rhinolophus sinicus bat embryonic fibroblast; BHK/hAPN, BHK cells stably expressing hAPN, the hCoV-229E receptor; 293/hACE2, 293 cells stably expressing hACE2, the SARS-CoV receptor; HeLa/hDPP4, HeLa cells stably expressing hDPP4, the MERS-CoV receptor. Experiments were done in triplicates and repeated at least three times. One representative is shown with error bars indicating SEM. c Binding of SARS-CoV S and SARS-CoV-2 S proteins to soluble hACE2. HEK293T cells transiently expressing SARS-CoV and SARS-CoV-2 S proteins were incubated with the soluble hACE2 on ice, followed by polyclonal goat anti-hACE2 antibody. Cells were analyzed by flow cytometry. The experiments were repeated at least three times. d Inhibition of SARS-CoV-2 S pseudovirion entry by soluble hACE2. SARS-CoV S, SARS-CoV-2 S, or VSV-G pseudovirions were pre-incubated with soluble hACE2, then mixture were added to 293/hACE2 cells. Cells were lysed 40 h later and pseudoviral transduction was measured. Experiments were done twice and one representative is shown. Error bars indicate SEM of technical triplicates. Source data are provided as a Source Data file."}

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