PMC:7094172 / 38513-40936
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T175 | 312-319 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T176 | 335-338 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
| T177 | 1983-1990 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T178 | 1991-1998 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T179 | 2379-2385 | Body_part | denotes | radius | http://purl.org/sig/ont/fma/fma23463 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T124 | 180-183 | Disease | denotes | NTD | http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075 |
| T126 | 232-235 | Disease | denotes | NTD | http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075 |
| T128 | 516-525 | Disease | denotes | hepatitis | http://purl.obolibrary.org/obo/MONDO_0002251 |
| T129 | 2066-2069 | Disease | denotes | NTD | http://purl.obolibrary.org/obo/MONDO_0008449|http://purl.obolibrary.org/obo/MONDO_0018075 |
| T131 | 2408-2417 | Disease | denotes | hepatitis | http://purl.obolibrary.org/obo/MONDO_0002251 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T288 | 192-194 | http://purl.obolibrary.org/obo/CLO_0050050 | denotes | S1 |
| T289 | 266-268 | http://purl.obolibrary.org/obo/CLO_0008922 | denotes | S2 |
| T290 | 266-268 | http://purl.obolibrary.org/obo/CLO_0050052 | denotes | S2 |
| T291 | 482-490 | http://purl.obolibrary.org/obo/CLO_0001658 | denotes | activity |
| T292 | 510-515 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T293 | 526-531 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
| T294 | 541-543 | http://purl.obolibrary.org/obo/CLO_0008933 | denotes | S5 |
| T295 | 612-614 | http://purl.obolibrary.org/obo/CLO_0050050 | denotes | S1 |
| T296 | 732-734 | http://purl.obolibrary.org/obo/CLO_0008922 | denotes | S2 |
| T297 | 732-734 | http://purl.obolibrary.org/obo/CLO_0050052 | denotes | S2 |
| T298 | 989-991 | http://purl.obolibrary.org/obo/CLO_0008285 | denotes | P1 |
| T299 | 2204-2206 | http://purl.obolibrary.org/obo/CLO_0007860 | denotes | MR |
| T300 | 2402-2407 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T301 | 2418-2423 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T362 | 266-268 | Chemical | denotes | S2 | http://purl.obolibrary.org/obo/CHEBI_29387 |
| T363 | 312-319 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T364 | 381-383 | Chemical | denotes | S3 | http://purl.obolibrary.org/obo/CHEBI_29388 |
| T365 | 428-437 | Chemical | denotes | antiviral | http://purl.obolibrary.org/obo/CHEBI_22587 |
| T366 | 467-469 | Chemical | denotes | S4 | http://purl.obolibrary.org/obo/CHEBI_29401 |
| T367 | 472-481 | Chemical | denotes | antiviral | http://purl.obolibrary.org/obo/CHEBI_22587 |
| T368 | 541-543 | Chemical | denotes | S5 | http://purl.obolibrary.org/obo/CHEBI_29386 |
| T369 | 732-734 | Chemical | denotes | S2 | http://purl.obolibrary.org/obo/CHEBI_29387 |
| T370 | 787-789 | Chemical | denotes | S3 | http://purl.obolibrary.org/obo/CHEBI_29388 |
| T371 | 989-991 | Chemical | denotes | P1 | http://purl.obolibrary.org/obo/CHEBI_60949 |
| T372 | 1258-1261 | Chemical | denotes | Lin | http://purl.obolibrary.org/obo/CHEBI_32386 |
| T373 | 1332-1335 | Chemical | denotes | Lin | http://purl.obolibrary.org/obo/CHEBI_32386 |
| T374 | 1345-1348 | Chemical | denotes | Lin | http://purl.obolibrary.org/obo/CHEBI_32386 |
| T375 | 1472-1480 | Chemical | denotes | reagents | http://purl.obolibrary.org/obo/CHEBI_33893 |
| T376 | 1508-1511 | Chemical | denotes | Lin | http://purl.obolibrary.org/obo/CHEBI_32386 |
| T377 | 1521-1524 | Chemical | denotes | Lin | http://purl.obolibrary.org/obo/CHEBI_32386 |
| T378 | 1585-1588 | Chemical | denotes | Lin | http://purl.obolibrary.org/obo/CHEBI_32386 |
| T379 | 1598-1601 | Chemical | denotes | Lin | http://purl.obolibrary.org/obo/CHEBI_32386 |
| T380 | 1983-1990 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T381 | 1991-1998 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T382 | 2204-2206 | Chemical | denotes | MR | http://purl.obolibrary.org/obo/CHEBI_74698 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T29 | 312-330 | http://purl.obolibrary.org/obo/GO_0050821 | denotes | protein sequesters |
| T30 | 312-330 | http://purl.obolibrary.org/obo/GO_0045185 | denotes | protein sequesters |
LitCovid-PD-HP
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T6 | 516-525 | Phenotype | denotes | hepatitis | http://purl.obolibrary.org/obo/HP_0012115 |
| T7 | 2408-2417 | Phenotype | denotes | hepatitis | http://purl.obolibrary.org/obo/HP_0012115 |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T310 | 0-32 | Sentence | denotes | Supporting Information Available |
| T311 | 33-823 | Sentence | denotes | The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jmedchem.9b01913.Dimeric conformation of MERS-CoV N-NTD (Figure S1); LibDock pose of dimeric MERS-CoV N-NTD with potent compounds (Figure S2); P3-induced aggregation of the MERS-CoV N protein sequesters the RNA-binding region on the N-CTD dimer (Figure S3); potential target sites for broad-spectrum antiviral compound development (Figure S4); antiviral activity of P3 against MHV (mouse hepatitis virus) (Figure S5); crystallographic data collection and refinement statistics (Table S1); pW43 docking pose with chemical structures, docking scores, and biochemical properties of 17 potential hits (Table S2); primers used for mutagenesis in this study (Table S3); molecular formula strings (PDF) |
| T312 | 824-868 | Sentence | denotes | Molecular Formula Strings_JMC_revision (CSV) |
| T313 | 870-892 | Sentence | denotes | Supplementary Material |
| T314 | 893-913 | Sentence | denotes | jm9b01913_si_001.pdf |
| T315 | 914-934 | Sentence | denotes | jm9b01913_si_002.csv |
| T316 | 936-951 | Sentence | denotes | Accession Codes |
| T317 | 952-1118 | Sentence | denotes | For PDB codes 6KL2 (native), 6KL5 (N-P1 complex), and 6KL6 (N-P3 complex), authors will release the atomic coordinates and experimental data upon article publication. |
| T318 | 1120-1140 | Sentence | denotes | Author Contributions |
| T319 | 1141-1215 | Sentence | denotes | ○ S.-M.L., S.-C.L., J.-N.H., and C.-k.C. contributed equally to this work. |
| T320 | 1217-1237 | Sentence | denotes | Author Contributions |
| T321 | 1238-1244 | Sentence | denotes | Jia N. |
| T322 | 1245-1257 | Sentence | denotes | Hsu, Shih C. |
| T323 | 1258-1270 | Sentence | denotes | Lin, Yong S. |
| T324 | 1271-1288 | Sentence | denotes | Wang, and Ming H. |
| T325 | 1289-1318 | Sentence | denotes | Hou designed research; Jia N. |
| T326 | 1319-1331 | Sentence | denotes | Hsu, Shih C. |
| T327 | 1332-1344 | Sentence | denotes | Lin, Shan M. |
| T328 | 1345-1358 | Sentence | denotes | Lin, Ching M. |
| T329 | 1359-1377 | Sentence | denotes | Chien, and Yong S. |
| T330 | 1378-1410 | Sentence | denotes | Wang performed research; Hung Y. |
| T331 | 1411-1419 | Sentence | denotes | Wu, U S. |
| T332 | 1420-1428 | Sentence | denotes | Jeng, K. |
| T333 | 1429-1451 | Sentence | denotes | Kehn-Hall, and Ming H. |
| T334 | 1452-1507 | Sentence | denotes | Hou contributed new reagents or analytic tools; Shih C. |
| T335 | 1508-1520 | Sentence | denotes | Lin, Shan M. |
| T336 | 1521-1534 | Sentence | denotes | Lin, Ching M. |
| T337 | 1535-1553 | Sentence | denotes | Chien, and Ming H. |
| T338 | 1554-1584 | Sentence | denotes | Hou analyzed the data; Shih C. |
| T339 | 1585-1597 | Sentence | denotes | Lin, Shan M. |
| T340 | 1598-1611 | Sentence | denotes | Lin, Chung K. |
| T341 | 1612-1627 | Sentence | denotes | Chang, Ching M. |
| T342 | 1628-1646 | Sentence | denotes | Chien, and Ming H. |
| T343 | 1647-1667 | Sentence | denotes | Hou wrote the paper. |
| T344 | 1669-1728 | Sentence | denotes | This work was supported by the MOST 106-2628-M-005-001-MY3. |
| T345 | 1730-1782 | Sentence | denotes | The authors declare no competing financial interest. |
| T346 | 1784-1799 | Sentence | denotes | Acknowledgments |
| T347 | 1800-1963 | Sentence | denotes | We thank the National Synchrotron Radiation Research Center (Taiwan) and the synchrotron radiation facility SPring-8 (Japan) for X-ray diffraction data collection. |
| T348 | 1964-1977 | Sentence | denotes | Abbreviations |
| T349 | 1978-2011 | Sentence | denotes | PPIs protein–protein interactions |
| T350 | 2012-2065 | Sentence | denotes | MERS-CoV Middle East Respiratory Syndrome Coronavirus |
| T351 | 2066-2087 | Sentence | denotes | NTD N-terminal domain |
| T352 | 2088-2109 | Sentence | denotes | CTD C-terminal domain |
| T353 | 2110-2124 | Sentence | denotes | N nucleocapsid |
| T354 | 2125-2147 | Sentence | denotes | β-CoVs betacoronavirus |
| T355 | 2148-2169 | Sentence | denotes | RNP ribonucleoprotein |
| T356 | 2170-2203 | Sentence | denotes | SAXS small-angle X-ray scattering |
| T357 | 2204-2228 | Sentence | denotes | MR molecular replacement |
| T358 | 2229-2253 | Sentence | denotes | BSA buried surface areas |
| T359 | 2254-2290 | Sentence | denotes | TPSA topological polar surface areas |
| T360 | 2291-2319 | Sentence | denotes | CC50 cytotoxic concentration |
| T361 | 2320-2348 | Sentence | denotes | EC50 effective concentration |
| T362 | 2349-2373 | Sentence | denotes | D max maximum dimension |
| T363 | 2374-2397 | Sentence | denotes | R g radius of gyration |
| T364 | 2398-2423 | Sentence | denotes | MHV mouse hepatitis virus |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 631 | 169-177 | Species | denotes | MERS-CoV | Tax:1335626 |
| 632 | 221-229 | Species | denotes | MERS-CoV | Tax:1335626 |
| 633 | 301-309 | Species | denotes | MERS-CoV | Tax:1335626 |
| 634 | 510-531 | Species | denotes | mouse hepatitis virus | Tax:11138 |
| 635 | 505-508 | Species | denotes | MHV | Tax:11138 |
| 637 | 1012-1016 | Chemical | denotes | N-P3 | |
| 639 | 2408-2423 | Disease | denotes | hepatitis virus | MESH:D006525 |