PMC:7094172 / 36339-36881 JSONTXT

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T605","span":{"begin":0,"end":4},"obj":"CL_6"},{"id":"T604","span":{"begin":106,"end":114},"obj":"SP_9"},{"id":"T603","span":{"begin":337,"end":344},"obj":"CHEBI:2511;CHEBI:2511"},{"id":"T602","span":{"begin":413,"end":416},"obj":"CHEBI:16526;CHEBI:16526"},{"id":"T601","span":{"begin":466,"end":474},"obj":"CHEBI:16842;CHEBI:16842"},{"id":"T600","span":{"begin":497,"end":511},"obj":"CHEBI:41688;CHEBI:41688"}],"text":"Vero E6 cells were seeded in 12-well plates and incubated overnight before the assays. Samples containing MERS-CoV were serially diluted 10× with MEM, added to the wells, and incubated for 1 h with agitation every 15 min. After incubation, the inocula were removed and washed with PBS. An overlay medium comprising 2× MEM and 1.5% (w/v) agarose (1:1) was added to the wells followed by incubation at 37 °C and 5% CO2 for 3 days. The plates were fixed with 10% (v/v) formalin containing 0.2% (w/v) crystal violet, and the plaques were counted."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T164","span":{"begin":8,"end":13},"obj":"Body_part"}],"attributes":[{"id":"A164","pred":"fma_id","subj":"T164","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Vero E6 cells were seeded in 12-well plates and incubated overnight before the assays. Samples containing MERS-CoV were serially diluted 10× with MEM, added to the wells, and incubated for 1 h with agitation every 15 min. After incubation, the inocula were removed and washed with PBS. An overlay medium comprising 2× MEM and 1.5% (w/v) agarose (1:1) was added to the wells followed by incubation at 37 °C and 5% CO2 for 3 days. The plates were fixed with 10% (v/v) formalin containing 0.2% (w/v) crystal violet, and the plaques were counted."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T272","span":{"begin":0,"end":13},"obj":"http://purl.obolibrary.org/obo/CLO_0051719"},{"id":"T273","span":{"begin":332,"end":335},"obj":"http://purl.obolibrary.org/obo/CLO_0050644"},{"id":"T274","span":{"begin":346,"end":349},"obj":"http://purl.obolibrary.org/obo/CLO_0053733"},{"id":"T275","span":{"begin":492,"end":495},"obj":"http://purl.obolibrary.org/obo/CLO_0050644"}],"text":"Vero E6 cells were seeded in 12-well plates and incubated overnight before the assays. Samples containing MERS-CoV were serially diluted 10× with MEM, added to the wells, and incubated for 1 h with agitation every 15 min. After incubation, the inocula were removed and washed with PBS. An overlay medium comprising 2× MEM and 1.5% (w/v) agarose (1:1) was added to the wells followed by incubation at 37 °C and 5% CO2 for 3 days. The plates were fixed with 10% (v/v) formalin containing 0.2% (w/v) crystal violet, and the plaques were counted."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T348","span":{"begin":337,"end":344},"obj":"Chemical"},{"id":"T349","span":{"begin":413,"end":416},"obj":"Chemical"},{"id":"T350","span":{"begin":497,"end":511},"obj":"Chemical"}],"attributes":[{"id":"A348","pred":"chebi_id","subj":"T348","obj":"http://purl.obolibrary.org/obo/CHEBI_2511"},{"id":"A349","pred":"chebi_id","subj":"T349","obj":"http://purl.obolibrary.org/obo/CHEBI_16526"},{"id":"A350","pred":"chebi_id","subj":"T350","obj":"http://purl.obolibrary.org/obo/CHEBI_41688"}],"text":"Vero E6 cells were seeded in 12-well plates and incubated overnight before the assays. Samples containing MERS-CoV were serially diluted 10× with MEM, added to the wells, and incubated for 1 h with agitation every 15 min. After incubation, the inocula were removed and washed with PBS. An overlay medium comprising 2× MEM and 1.5% (w/v) agarose (1:1) was added to the wells followed by incubation at 37 °C and 5% CO2 for 3 days. The plates were fixed with 10% (v/v) formalin containing 0.2% (w/v) crystal violet, and the plaques were counted."}

{"project":"LitCovid-PD-HP","denotations":[{"id":"T5","span":{"begin":198,"end":207},"obj":"Phenotype"}],"attributes":[{"id":"A5","pred":"hp_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/HP_0000713"}],"text":"Vero E6 cells were seeded in 12-well plates and incubated overnight before the assays. Samples containing MERS-CoV were serially diluted 10× with MEM, added to the wells, and incubated for 1 h with agitation every 15 min. After incubation, the inocula were removed and washed with PBS. An overlay medium comprising 2× MEM and 1.5% (w/v) agarose (1:1) was added to the wells followed by incubation at 37 °C and 5% CO2 for 3 days. The plates were fixed with 10% (v/v) formalin containing 0.2% (w/v) crystal violet, and the plaques were counted."}

{"project":"LitCovid-sentences","denotations":[{"id":"T288","span":{"begin":0,"end":86},"obj":"Sentence"},{"id":"T289","span":{"begin":87,"end":221},"obj":"Sentence"},{"id":"T290","span":{"begin":222,"end":285},"obj":"Sentence"},{"id":"T291","span":{"begin":286,"end":428},"obj":"Sentence"},{"id":"T292","span":{"begin":429,"end":542},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Vero E6 cells were seeded in 12-well plates and incubated overnight before the assays. Samples containing MERS-CoV were serially diluted 10× with MEM, added to the wells, and incubated for 1 h with agitation every 15 min. After incubation, the inocula were removed and washed with PBS. An overlay medium comprising 2× MEM and 1.5% (w/v) agarose (1:1) was added to the wells followed by incubation at 37 °C and 5% CO2 for 3 days. The plates were fixed with 10% (v/v) formalin containing 0.2% (w/v) crystal violet, and the plaques were counted."}

{"project":"LitCovid-PubTator","denotations":[{"id":"576","span":{"begin":106,"end":114},"obj":"Species"},{"id":"577","span":{"begin":281,"end":284},"obj":"Chemical"},{"id":"578","span":{"begin":337,"end":344},"obj":"Chemical"},{"id":"579","span":{"begin":413,"end":416},"obj":"Chemical"},{"id":"580","span":{"begin":466,"end":474},"obj":"Chemical"},{"id":"581","span":{"begin":497,"end":511},"obj":"Chemical"},{"id":"582","span":{"begin":198,"end":207},"obj":"Disease"},{"id":"583","span":{"begin":5,"end":7},"obj":"CellLine"}],"attributes":[{"id":"A576","pred":"tao:has_database_id","subj":"576","obj":"Tax:1335626"},{"id":"A578","pred":"tao:has_database_id","subj":"578","obj":"MESH:D012685"},{"id":"A579","pred":"tao:has_database_id","subj":"579","obj":"MESH:D002245"},{"id":"A580","pred":"tao:has_database_id","subj":"580","obj":"MESH:D005557"},{"id":"A581","pred":"tao:has_database_id","subj":"581","obj":"MESH:D005840"},{"id":"A582","pred":"tao:has_database_id","subj":"582","obj":"MESH:D011595"},{"id":"A583","pred":"tao:has_database_id","subj":"583","obj":"CVCL:4582"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Vero E6 cells were seeded in 12-well plates and incubated overnight before the assays. Samples containing MERS-CoV were serially diluted 10× with MEM, added to the wells, and incubated for 1 h with agitation every 15 min. After incubation, the inocula were removed and washed with PBS. An overlay medium comprising 2× MEM and 1.5% (w/v) agarose (1:1) was added to the wells followed by incubation at 37 °C and 5% CO2 for 3 days. The plates were fixed with 10% (v/v) formalin containing 0.2% (w/v) crystal violet, and the plaques were counted."}