PMC:7094172 / 31249-31656 JSONTXT

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T510","span":{"begin":34,"end":43},"obj":"CHEBI:75958;CHEBI:75958"},{"id":"T509","span":{"begin":85,"end":93},"obj":"SP_9"},{"id":"T508","span":{"begin":120,"end":134},"obj":"CHEBI:64276;CHEBI:64276"},{"id":"T507","span":{"begin":267,"end":271},"obj":"CHEBI:9754;CHEBI:9754"},{"id":"T506","span":{"begin":272,"end":275},"obj":"CHEBI:17883;CHEBI:17883"},{"id":"T505","span":{"begin":338,"end":360},"obj":"CHEBI:8984;CHEBI:8984"},{"id":"T504","span":{"begin":397,"end":400},"obj":"CHEBI:8984;CHEBI:8984"}],"text":"Chemical Cross-Link Assay\nProtein solutions containing 40 μM of wild-type or mutated MERS-CoV N-NTD were incubated with glutaraldehyde at a final concentration of 1% v/v. The reaction was conducted at room temperature for 10 min and quenched with the addition of 1 M Tris-HCl (pH 7.5). The samples were then stored on ice and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T145","span":{"begin":26,"end":33},"obj":"Body_part"}],"attributes":[{"id":"A145","pred":"fma_id","subj":"T145","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"Chemical Cross-Link Assay\nProtein solutions containing 40 μM of wild-type or mutated MERS-CoV N-NTD were incubated with glutaraldehyde at a final concentration of 1% v/v. The reaction was conducted at room temperature for 10 min and quenched with the addition of 1 M Tris-HCl (pH 7.5). The samples were then stored on ice and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T111","span":{"begin":96,"end":99},"obj":"Disease"}],"attributes":[{"id":"A111","pred":"mondo_id","subj":"T111","obj":"http://purl.obolibrary.org/obo/MONDO_0008449"},{"id":"A112","pred":"mondo_id","subj":"T111","obj":"http://purl.obolibrary.org/obo/MONDO_0018075"}],"text":"Chemical Cross-Link Assay\nProtein solutions containing 40 μM of wild-type or mutated MERS-CoV N-NTD were incubated with glutaraldehyde at a final concentration of 1% v/v. The reaction was conducted at room temperature for 10 min and quenched with the addition of 1 M Tris-HCl (pH 7.5). The samples were then stored on ice and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T234","span":{"begin":138,"end":139},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Chemical Cross-Link Assay\nProtein solutions containing 40 μM of wild-type or mutated MERS-CoV N-NTD were incubated with glutaraldehyde at a final concentration of 1% v/v. The reaction was conducted at room temperature for 10 min and quenched with the addition of 1 M Tris-HCl (pH 7.5). The samples were then stored on ice and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T281","span":{"begin":26,"end":33},"obj":"Chemical"},{"id":"T282","span":{"begin":120,"end":134},"obj":"Chemical"},{"id":"T283","span":{"begin":267,"end":271},"obj":"Chemical"},{"id":"T284","span":{"begin":272,"end":275},"obj":"Chemical"},{"id":"T285","span":{"begin":338,"end":360},"obj":"Chemical"},{"id":"T286","span":{"begin":338,"end":344},"obj":"Chemical"},{"id":"T287","span":{"begin":345,"end":360},"obj":"Chemical"},{"id":"T288","span":{"begin":345,"end":352},"obj":"Chemical"},{"id":"T289","span":{"begin":353,"end":360},"obj":"Chemical"},{"id":"T290","span":{"begin":361,"end":375},"obj":"Chemical"},{"id":"T293","span":{"begin":397,"end":400},"obj":"Chemical"}],"attributes":[{"id":"A281","pred":"chebi_id","subj":"T281","obj":"http://purl.obolibrary.org/obo/CHEBI_16541"},{"id":"A282","pred":"chebi_id","subj":"T282","obj":"http://purl.obolibrary.org/obo/CHEBI_64276"},{"id":"A283","pred":"chebi_id","subj":"T283","obj":"http://purl.obolibrary.org/obo/CHEBI_9754"},{"id":"A284","pred":"chebi_id","subj":"T284","obj":"http://purl.obolibrary.org/obo/CHEBI_17883"},{"id":"A285","pred":"chebi_id","subj":"T285","obj":"http://purl.obolibrary.org/obo/CHEBI_8984"},{"id":"A286","pred":"chebi_id","subj":"T286","obj":"http://purl.obolibrary.org/obo/CHEBI_26708"},{"id":"A287","pred":"chebi_id","subj":"T287","obj":"http://purl.obolibrary.org/obo/CHEBI_23872"},{"id":"A288","pred":"chebi_id","subj":"T288","obj":"http://purl.obolibrary.org/obo/CHEBI_23870"},{"id":"A289","pred":"chebi_id","subj":"T289","obj":"http://purl.obolibrary.org/obo/CHEBI_16189"},{"id":"A290","pred":"chebi_id","subj":"T290","obj":"http://purl.obolibrary.org/obo/CHEBI_51135"},{"id":"A291","pred":"chebi_id","subj":"T290","obj":"http://purl.obolibrary.org/obo/CHEBI_53656"},{"id":"A292","pred":"chebi_id","subj":"T290","obj":"http://purl.obolibrary.org/obo/CHEBI_60766"},{"id":"A293","pred":"chebi_id","subj":"T293","obj":"http://purl.obolibrary.org/obo/CHEBI_8984"}],"text":"Chemical Cross-Link Assay\nProtein solutions containing 40 μM of wild-type or mutated MERS-CoV N-NTD were incubated with glutaraldehyde at a final concentration of 1% v/v. The reaction was conducted at room temperature for 10 min and quenched with the addition of 1 M Tris-HCl (pH 7.5). The samples were then stored on ice and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T239","span":{"begin":0,"end":25},"obj":"Sentence"},{"id":"T240","span":{"begin":26,"end":170},"obj":"Sentence"},{"id":"T241","span":{"begin":171,"end":285},"obj":"Sentence"},{"id":"T242","span":{"begin":286,"end":407},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Chemical Cross-Link Assay\nProtein solutions containing 40 μM of wild-type or mutated MERS-CoV N-NTD were incubated with glutaraldehyde at a final concentration of 1% v/v. The reaction was conducted at room temperature for 10 min and quenched with the addition of 1 M Tris-HCl (pH 7.5). The samples were then stored on ice and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"494","span":{"begin":85,"end":93},"obj":"Species"},{"id":"495","span":{"begin":120,"end":134},"obj":"Chemical"},{"id":"496","span":{"begin":267,"end":275},"obj":"Chemical"},{"id":"497","span":{"begin":338,"end":360},"obj":"Chemical"},{"id":"498","span":{"begin":361,"end":375},"obj":"Chemical"},{"id":"499","span":{"begin":397,"end":400},"obj":"Chemical"}],"attributes":[{"id":"A494","pred":"tao:has_database_id","subj":"494","obj":"Tax:1335626"},{"id":"A495","pred":"tao:has_database_id","subj":"495","obj":"MESH:D005976"},{"id":"A497","pred":"tao:has_database_id","subj":"497","obj":"MESH:D012967"},{"id":"A498","pred":"tao:has_database_id","subj":"498","obj":"MESH:C016679"},{"id":"A499","pred":"tao:has_database_id","subj":"499","obj":"MESH:D012967"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Chemical Cross-Link Assay\nProtein solutions containing 40 μM of wild-type or mutated MERS-CoV N-NTD were incubated with glutaraldehyde at a final concentration of 1% v/v. The reaction was conducted at room temperature for 10 min and quenched with the addition of 1 M Tris-HCl (pH 7.5). The samples were then stored on ice and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)."}