PMC:7094172 / 19381-20037 JSONTXT

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T731","span":{"begin":38,"end":47},"obj":"CHEBI:35222;CHEBI:35222"},{"id":"T730","span":{"begin":56,"end":64},"obj":"SP_9"},{"id":"T729","span":{"begin":105,"end":113},"obj":"SP_9"},{"id":"T728","span":{"begin":254,"end":258},"obj":"SP_9"},{"id":"T727","span":{"begin":466,"end":474},"obj":"SP_9"},{"id":"T726","span":{"begin":475,"end":495},"obj":"PG_4"},{"id":"T725","span":{"begin":531,"end":551},"obj":"PG_4"},{"id":"T724","span":{"begin":552,"end":563},"obj":"GO:0010467"},{"id":"T723","span":{"begin":621,"end":627},"obj":"GO:0005634"},{"id":"T722","span":{"begin":651,"end":655},"obj":"CHEBI:51231;CHEBI:51231"}],"text":"Figure 4 Compound P3 was a potential inhibitor against MERS-CoV. (A, B) Viral titers (A) and RNA (B) of MERS-CoV measured by plaque assay and RT-qPCR, respectively, decreased after P3 treatment for 48 h. Relative RNA levels were determined by comparing MERS alone at each time point. GAPDH RNA was the internal control. All values are presented as mean ± SE (standard error of mean). One-way Anova was used for statistics (*p \u003c 0.05, **p \u003c 0.01, ***p \u003c 0.001). (C) MERS-CoV nucleocapsid protein decreased after 48 h P3 treatment. Nucleocapsid protein expressions (red) were examined under a confocal microscope at ×680. Nuclei were stained blue with DAPI."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T112","span":{"begin":94,"end":97},"obj":"Body_part"},{"id":"T113","span":{"begin":214,"end":217},"obj":"Body_part"},{"id":"T114","span":{"begin":291,"end":294},"obj":"Body_part"},{"id":"T115","span":{"begin":488,"end":495},"obj":"Body_part"},{"id":"T116","span":{"begin":544,"end":551},"obj":"Body_part"}],"attributes":[{"id":"A112","pred":"fma_id","subj":"T112","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A113","pred":"fma_id","subj":"T113","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A114","pred":"fma_id","subj":"T114","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A115","pred":"fma_id","subj":"T115","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A116","pred":"fma_id","subj":"T116","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"Figure 4 Compound P3 was a potential inhibitor against MERS-CoV. (A, B) Viral titers (A) and RNA (B) of MERS-CoV measured by plaque assay and RT-qPCR, respectively, decreased after P3 treatment for 48 h. Relative RNA levels were determined by comparing MERS alone at each time point. GAPDH RNA was the internal control. All values are presented as mean ± SE (standard error of mean). One-way Anova was used for statistics (*p \u003c 0.05, **p \u003c 0.01, ***p \u003c 0.001). (C) MERS-CoV nucleocapsid protein decreased after 48 h P3 treatment. Nucleocapsid protein expressions (red) were examined under a confocal microscope at ×680. Nuclei were stained blue with DAPI."}

{"project":"LitCovid_AGAC","denotations":[{"id":"p35853s3","span":{"begin":466,"end":470},"obj":"Protein"},{"id":"p35853s7","span":{"begin":488,"end":495},"obj":"MPA"},{"id":"p35853s8","span":{"begin":496,"end":505},"obj":"NegReg"}],"text":"Figure 4 Compound P3 was a potential inhibitor against MERS-CoV. (A, B) Viral titers (A) and RNA (B) of MERS-CoV measured by plaque assay and RT-qPCR, respectively, decreased after P3 treatment for 48 h. Relative RNA levels were determined by comparing MERS alone at each time point. GAPDH RNA was the internal control. All values are presented as mean ± SE (standard error of mean). One-way Anova was used for statistics (*p \u003c 0.05, **p \u003c 0.01, ***p \u003c 0.001). (C) MERS-CoV nucleocapsid protein decreased after 48 h P3 treatment. Nucleocapsid protein expressions (red) were examined under a confocal microscope at ×680. Nuclei were stained blue with DAPI."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T69","span":{"begin":356,"end":358},"obj":"Disease"}],"attributes":[{"id":"A69","pred":"mondo_id","subj":"T69","obj":"http://purl.obolibrary.org/obo/MONDO_0002125"}],"text":"Figure 4 Compound P3 was a potential inhibitor against MERS-CoV. (A, B) Viral titers (A) and RNA (B) of MERS-CoV measured by plaque assay and RT-qPCR, respectively, decreased after P3 treatment for 48 h. Relative RNA levels were determined by comparing MERS alone at each time point. GAPDH RNA was the internal control. All values are presented as mean ± SE (standard error of mean). One-way Anova was used for statistics (*p \u003c 0.05, **p \u003c 0.01, ***p \u003c 0.001). (C) MERS-CoV nucleocapsid protein decreased after 48 h P3 treatment. Nucleocapsid protein expressions (red) were examined under a confocal microscope at ×680. Nuclei were stained blue with DAPI."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T137","span":{"begin":26,"end":27},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T138","span":{"begin":67,"end":68},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T139","span":{"begin":70,"end":71},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T140","span":{"begin":87,"end":88},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T141","span":{"begin":99,"end":100},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T142","span":{"begin":199,"end":201},"obj":"http://purl.obolibrary.org/obo/CLO_0001382"},{"id":"T143","span":{"begin":512,"end":514},"obj":"http://purl.obolibrary.org/obo/CLO_0001382"},{"id":"T144","span":{"begin":590,"end":591},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Figure 4 Compound P3 was a potential inhibitor against MERS-CoV. (A, B) Viral titers (A) and RNA (B) of MERS-CoV measured by plaque assay and RT-qPCR, respectively, decreased after P3 treatment for 48 h. Relative RNA levels were determined by comparing MERS alone at each time point. GAPDH RNA was the internal control. All values are presented as mean ± SE (standard error of mean). One-way Anova was used for statistics (*p \u003c 0.05, **p \u003c 0.01, ***p \u003c 0.001). (C) MERS-CoV nucleocapsid protein decreased after 48 h P3 treatment. Nucleocapsid protein expressions (red) were examined under a confocal microscope at ×680. Nuclei were stained blue with DAPI."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T157","span":{"begin":38,"end":47},"obj":"Chemical"},{"id":"T158","span":{"begin":356,"end":358},"obj":"Chemical"},{"id":"T159","span":{"begin":488,"end":495},"obj":"Chemical"},{"id":"T160","span":{"begin":544,"end":551},"obj":"Chemical"},{"id":"T161","span":{"begin":651,"end":655},"obj":"Chemical"}],"attributes":[{"id":"A157","pred":"chebi_id","subj":"T157","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A158","pred":"chebi_id","subj":"T158","obj":"http://purl.obolibrary.org/obo/CHEBI_74813"},{"id":"A159","pred":"chebi_id","subj":"T159","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A160","pred":"chebi_id","subj":"T160","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A161","pred":"chebi_id","subj":"T161","obj":"http://purl.obolibrary.org/obo/CHEBI_51231"}],"text":"Figure 4 Compound P3 was a potential inhibitor against MERS-CoV. (A, B) Viral titers (A) and RNA (B) of MERS-CoV measured by plaque assay and RT-qPCR, respectively, decreased after P3 treatment for 48 h. Relative RNA levels were determined by comparing MERS alone at each time point. GAPDH RNA was the internal control. All values are presented as mean ± SE (standard error of mean). One-way Anova was used for statistics (*p \u003c 0.05, **p \u003c 0.01, ***p \u003c 0.001). (C) MERS-CoV nucleocapsid protein decreased after 48 h P3 treatment. Nucleocapsid protein expressions (red) were examined under a confocal microscope at ×680. Nuclei were stained blue with DAPI."}

{"project":"LitCovid-sentences","denotations":[{"id":"T153","span":{"begin":0,"end":204},"obj":"Sentence"},{"id":"T154","span":{"begin":205,"end":284},"obj":"Sentence"},{"id":"T155","span":{"begin":285,"end":320},"obj":"Sentence"},{"id":"T156","span":{"begin":321,"end":384},"obj":"Sentence"},{"id":"T157","span":{"begin":385,"end":530},"obj":"Sentence"},{"id":"T158","span":{"begin":531,"end":620},"obj":"Sentence"},{"id":"T159","span":{"begin":621,"end":656},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Figure 4 Compound P3 was a potential inhibitor against MERS-CoV. (A, B) Viral titers (A) and RNA (B) of MERS-CoV measured by plaque assay and RT-qPCR, respectively, decreased after P3 treatment for 48 h. Relative RNA levels were determined by comparing MERS alone at each time point. GAPDH RNA was the internal control. All values are presented as mean ± SE (standard error of mean). One-way Anova was used for statistics (*p \u003c 0.05, **p \u003c 0.01, ***p \u003c 0.001). (C) MERS-CoV nucleocapsid protein decreased after 48 h P3 treatment. Nucleocapsid protein expressions (red) were examined under a confocal microscope at ×680. Nuclei were stained blue with DAPI."}

{"project":"LitCovid-PubTator","denotations":[{"id":"322","span":{"begin":285,"end":290},"obj":"Gene"},{"id":"323","span":{"begin":56,"end":64},"obj":"Species"},{"id":"324","span":{"begin":105,"end":113},"obj":"Species"},{"id":"325","span":{"begin":466,"end":474},"obj":"Species"},{"id":"326","span":{"begin":517,"end":519},"obj":"Chemical"},{"id":"327","span":{"begin":651,"end":655},"obj":"Chemical"}],"attributes":[{"id":"A322","pred":"tao:has_database_id","subj":"322","obj":"Gene:2597"},{"id":"A323","pred":"tao:has_database_id","subj":"323","obj":"Tax:1335626"},{"id":"A324","pred":"tao:has_database_id","subj":"324","obj":"Tax:1335626"},{"id":"A325","pred":"tao:has_database_id","subj":"325","obj":"Tax:1335626"},{"id":"A327","pred":"tao:has_database_id","subj":"327","obj":"MESH:C007293"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Figure 4 Compound P3 was a potential inhibitor against MERS-CoV. (A, B) Viral titers (A) and RNA (B) of MERS-CoV measured by plaque assay and RT-qPCR, respectively, decreased after P3 treatment for 48 h. Relative RNA levels were determined by comparing MERS alone at each time point. GAPDH RNA was the internal control. All values are presented as mean ± SE (standard error of mean). One-way Anova was used for statistics (*p \u003c 0.05, **p \u003c 0.01, ***p \u003c 0.001). (C) MERS-CoV nucleocapsid protein decreased after 48 h P3 treatment. Nucleocapsid protein expressions (red) were examined under a confocal microscope at ×680. Nuclei were stained blue with DAPI."}