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PMC:7091888 / 9897-13134 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
329 45-49 Gene denotes ACE2 Gene:59272
330 185-189 Gene denotes ACE2 Gene:59272
331 191-196 Gene denotes hACE2 Gene:59272
332 225-230 Gene denotes hDPP4 Gene:1803
333 291-301 Gene denotes hACE2/293T Gene:59272
334 324-329 Gene denotes hACE2 Gene:59272
335 353-358 Gene denotes hDPP4 Gene:1803
336 409-419 Gene denotes hDPP4/293T Gene:1803
337 442-447 Gene denotes hDPP4 Gene:1803
338 469-474 Gene denotes hACE2 Gene:59272
339 730-735 Gene denotes hACE2 Gene:59272
340 748-758 Gene denotes hACE2/293T Gene:59272
341 819-824 Gene denotes hACE2 Gene:59272
342 953-958 Gene denotes hACE2 Gene:59272
343 1098-1103 Gene denotes hACE2 Gene:59272
344 1498-1503 Gene denotes hACE2 Gene:59272
345 1516-1526 Gene denotes hACE2/293T Gene:59272
346 1715-1720 Gene denotes hACE2 Gene:59272
347 1750-1755 Gene denotes hACE2 Gene:59272
348 1883-1888 Gene denotes hACE2 Gene:59272
349 1898-1903 Gene denotes hACE2 Gene:59272
350 1948-1953 Gene denotes hACE2 Gene:59272
351 2161-2166 Gene denotes hACE2 Gene:59272
352 2550-2555 Gene denotes hACE2 Gene:59272
353 2616-2621 Gene denotes hDPP4 Gene:1803
354 2729-2734 Gene denotes hACE2 Gene:59272
355 2785-2790 Gene denotes hACE2 Gene:59272
356 13-23 Species denotes SARS-CoV-2 Tax:2697049
357 39-44 Species denotes human Tax:9606
358 179-184 Species denotes human Tax:9606
359 688-698 Species denotes SARS-CoV-2 Tax:2697049
360 778-788 Species denotes SARS-CoV-2 Tax:2697049
361 923-931 Species denotes SARS-CoV Tax:694009
362 1456-1466 Species denotes SARS-CoV-2 Tax:2697049
363 1534-1544 Species denotes SARS-CoV-2 Tax:2697049
364 1569-1577 Species denotes SARS-CoV Tax:694009
365 2127-2137 Species denotes SARS-CoV-2 Tax:2697049
366 2198-2208 Species denotes SARS-CoV-2 Tax:2697049
367 2284-2292 Species denotes SARS-CoV Tax:694009
368 2301-2309 Species denotes MERS-CoV Tax:1335626
369 2501-2511 Species denotes SARS-CoV-2 Tax:2697049
370 2527-2535 Species denotes SARS-CoV Tax:694009
371 2695-2705 Species denotes SARS-CoV-2 Tax:2697049
372 2767-2777 Species denotes SARS-CoV-2 Tax:2697049
373 1213-1218 Species denotes Human Tax:9606
374 1687-1692 Species denotes human Tax:9606
375 2232-2237 Gene denotes hDPP4 Gene:1803
376 1664-1668 Chemical denotes FITC MESH:D016650
377 1779-1794 Chemical denotes Alexa-Fluor 647 MESH:C569686
378 1991-2020 Chemical denotes 4’,6-diamidino-2-phenylindole MESH:C007293
379 2022-2026 Chemical denotes DAPI MESH:C007293
380 144-148 CellLine denotes 293T CVCL:0063
381 285-289 CellLine denotes 293T CVCL:0063
382 403-407 CellLine denotes 293T CVCL:0063
383 825-829 CellLine denotes 293T CVCL:0063
384 881-885 CellLine denotes 293T CVCL:0063
385 959-963 CellLine denotes 293T CVCL:0063
386 1017-1021 CellLine denotes 293T CVCL:0063
387 1104-1108 CellLine denotes 293T CVCL:0063
388 1170-1174 CellLine denotes 293T CVCL:0063
389 1954-1958 CellLine denotes 293T CVCL:0063
390 2735-2739 CellLine denotes 293T CVCL:0063
391 2791-2795 CellLine denotes 293T CVCL:0063

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T61 119-123 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T62 149-154 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T63 303-308 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T64 421-426 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T65 505-510 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T66 714-718 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T67 766-770 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T68 793-800 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T69 830-835 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T70 886-891 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T71 936-943 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T72 964-969 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T73 1022-1027 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T74 1074-1081 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T75 1109-1114 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T76 1175-1180 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T77 1219-1222 Body_part denotes IgG http://purl.org/sig/ont/fma/fma62872
T78 1253-1260 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T79 1271-1276 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T80 1308-1313 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T81 1482-1486 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T82 1527-1532 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T83 1549-1556 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T84 1582-1589 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T85 1693-1696 Body_part denotes IgG http://purl.org/sig/ont/fma/fma62872
T86 1697-1705 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T87 1756-1764 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T88 1813-1821 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T89 1859-1866 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T90 1959-1964 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T91 2142-2149 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T92 2314-2322 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T93 2377-2384 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T94 2556-2563 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T95 2622-2629 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T96 2710-2717 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T97 2740-2745 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T98 2796-2801 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T99 2895-2902 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T100 2913-2918 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T101 2950-2955 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 2035-2040 Body_part denotes Scale http://purl.obolibrary.org/obo/UBERON_0002542

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T178 13-21 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T179 13-17 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T180 688-696 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T181 688-692 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T182 778-786 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T183 778-782 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T184 923-931 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T185 923-927 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T186 1456-1464 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T187 1456-1460 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T188 1534-1542 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T189 1534-1538 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T190 1569-1577 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T191 1569-1573 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T192 2127-2135 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T193 2127-2131 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T194 2198-2206 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T195 2198-2202 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T196 2284-2292 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T197 2284-2288 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T198 2501-2509 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T199 2501-2505 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T200 2527-2535 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T201 2527-2531 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T202 2695-2703 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T203 2695-2699 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T204 2767-2775 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T205 2767-2771 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T140 39-44 http://purl.obolibrary.org/obo/NCBITaxon_9606 denotes human
T141 60-61 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T142 119-129 http://purl.obolibrary.org/obo/CLO_0000031 denotes cell lines
T143 144-148 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T144 144-148 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T145 144-148 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T146 149-154 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T147 179-184 http://purl.obolibrary.org/obo/NCBITaxon_9606 denotes human
T148 285-289 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T149 285-289 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T150 285-289 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T151 297-301 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T152 297-301 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T153 297-301 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T154 303-308 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T155 403-407 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T156 403-407 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T157 403-407 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T158 415-419 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T159 415-419 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T160 415-419 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T161 421-426 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T162 505-510 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T163 656-657 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T164 714-718 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T165 754-758 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T166 754-758 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T167 754-758 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T168 766-776 http://purl.obolibrary.org/obo/CLO_0000031 denotes cell lines
T169 825-829 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T170 825-829 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T171 825-829 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T172 830-835 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T173 837-838 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T174 881-885 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T175 881-885 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T176 881-885 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T177 886-891 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T178 959-963 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T179 959-963 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T180 959-963 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T181 964-969 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T182 971-972 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T183 1017-1021 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T184 1017-1021 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T185 1017-1021 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T186 1022-1027 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T187 1104-1108 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T188 1104-1108 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T189 1104-1108 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T190 1109-1114 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T191 1116-1117 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T192 1170-1174 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T193 1170-1174 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T194 1170-1174 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T195 1175-1180 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T196 1213-1222 http://purl.obolibrary.org/obo/CLO_0053695 denotes Human IgG
T197 1223-1225 http://purl.obolibrary.org/obo/CLO_0052676 denotes Fc
T198 1232-1234 http://purl.obolibrary.org/obo/CLO_0052676 denotes Fc
T199 1271-1276 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T200 1308-1313 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T201 1356-1357 http://purl.obolibrary.org/obo/CLO_0001021 denotes b
T202 1482-1486 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T203 1522-1526 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T204 1522-1526 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T205 1522-1526 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T206 1527-1532 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T207 1628-1629 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T208 1669-1676 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T209 1677-1681 http://purl.obolibrary.org/obo/NCBITaxon_9925 denotes goat
T210 1687-1696 http://purl.obolibrary.org/obo/CLO_0053695 denotes human IgG
T211 1738-1739 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T212 1740-1744 http://purl.obolibrary.org/obo/NCBITaxon_9925 denotes goat
T213 1795-1802 http://purl.obolibrary.org/obo/CLO_0007225 denotes labeled
T214 1808-1812 http://purl.obolibrary.org/obo/NCBITaxon_9925 denotes goat
T215 1823-1830 http://purl.obolibrary.org/obo/CLO_0008776 denotes red) (1
T216 1823-1830 http://purl.obolibrary.org/obo/CLO_0008777 denotes red) (1
T217 1837-1839 http://purl.obolibrary.org/obo/CLO_0052676 denotes Fc
T218 1954-1958 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T219 1954-1958 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T220 1954-1958 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T221 1959-1964 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T222 2356-2362 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tested
T223 2735-2739 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T224 2735-2739 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T225 2735-2739 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T226 2740-2745 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T227 2791-2795 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T228 2791-2795 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T229 2791-2795 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T230 2796-2801 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T231 2913-2918 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T232 2950-2955 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T47 793-800 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T48 936-943 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T49 1074-1081 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T50 1253-1260 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T51 1549-1556 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T52 1582-1589 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T53 1664-1668 Chemical denotes FITC http://purl.obolibrary.org/obo/CHEBI_37926
T54 1785-1790 Chemical denotes Fluor http://purl.obolibrary.org/obo/CHEBI_24061
T55 1859-1866 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T56 2008-2020 Chemical denotes phenylindole http://purl.obolibrary.org/obo/CHEBI_48559
T57 2022-2026 Chemical denotes DAPI http://purl.obolibrary.org/obo/CHEBI_51231
T58 2142-2149 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T59 2314-2322 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T60 2377-2384 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T61 2556-2563 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T62 2622-2629 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T63 2710-2717 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T64 2895-2902 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T52 490-547 Sentence denotes Mock-incubated cells (gray shading) were used as control.
T53 548-777 Sentence denotes Representative images and median fluorescence intensity (MFI) ± standard error (s.e.m.) were shown (n = 4). b, c Flow cytometry analysis of SARS-CoV-2 RBD binding to cell-associated hACE2 receptor in hACE2/293T stable cell lines.
T54 778-922 Sentence denotes SARS-CoV-2 RBD protein bound strongly to hACE2/293T cells (b (left panel, red line)), but not to hDPP4/293T cells (c (left panel, violet line)).
T55 923-1060 Sentence denotes SARS-CoV RBD protein bound to hACE2/293T cells (b (middle panel, red line)), but not to hDPP4/293T cells (c (middle panel, violet line)).
T56 1061-1212 Sentence denotes MERS-CoV RBD protein did not bind to hACE2/293T cells (b (right panel, red line)), but rather bound to hDPP4/293T cells (c (right panel, violet line)).
T57 1213-1362 Sentence denotes Human IgG Fc (hIgG-Fc, hereinafter hFc) protein-incubated cells (blue line) and mock-incubated cells (gray shading) were included as controls (b, c).
T58 1363-1533 Sentence denotes Representative images and MFI ± s.e.m. were shown (n = 4). d Immunofluorescence detection of SARS-CoV-2 RBD binding to cell-associated hACE2 receptor in hACE2/293T cells.
T59 1534-1836 Sentence denotes SARS-CoV-2 RBD protein (green) and SARS-CoV RBD protein (green), each of which was fused with a C-terminal hFc, were stained with FITC-labeled goat anti-human IgG antibody (1:500). hACE2 was stained with a goat-anti-hACE2 antibody (5 μg/ml) and Alexa-Fluor 647-labeled anti-goat antibody (red) (1:200).
T60 1837-1965 Sentence denotes Fc-fused MERS-CoV RBD protein did not bind to hACE2, so only hACE2 (red), but not RBD (green), was detected in hACE2/293T cells.
T61 1966-2034 Sentence denotes Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, blue).
T62 2035-2045 Sentence denotes Scale bar:
T63 2046-2052 Sentence denotes 10 μm.
T64 2053-2193 Sentence denotes Representative images are shown. e Detection of dose-dependent binding of SARS-CoV-2 RBD protein to soluble hACE2 (sACE2) receptor by ELISA.
T65 2194-2363 Sentence denotes The SARS-CoV-2 RBD binding to soluble hDPP4 (sDPP4) receptor (f), and the binding of both SARS-CoV RBD and MERS-CoV RBD proteins to sACE2 (e), or sDPP4 (f), were tested.
T66 2364-2385 Sentence denotes Control: hFc protein.
T67 2386-2435 Sentence denotes Data are presented as mean A450 ± s.e.m. (n = 4).
T68 2436-2755 Sentence denotes 50% effective dose (EC50) was calculated for the binding between SARS-CoV-2 RBD (black) or SARS-CoV RBD (red) and hACE2 protein (e, sACE2), or the binding between MERS-CoV RBD and hDPP4 protein (sDPP4, green) (f). g–i Flow cytometry analysis of inhibition of SARS-CoV-2 RBD protein binding to hACE2/293T cells by sACE2.
T69 2756-3004 Sentence denotes Binding of SARS-CoV-2 RBD to hACE2/293T cells (g, h, green line) was blocked by sACE2 (g, black line), but not by sDPP4 (h, red line). hFc protein-incubated cells (blue line) and mock-incubated cells (gray shading) were included as controls (g, h).
T70 3005-3138 Sentence denotes Representative images are shown. i The blocking ability of sACE2 or sDPP4, as described above, was expressed as MFI ± s.e.m. (n = 4).
T71 3139-3177 Sentence denotes Low MFI correlates with high blockage.
T72 3178-3237 Sentence denotes Experiments were repeated twice and yielded similar results