PMC:7091888 / 27874-28130 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"1099","span":{"begin":56,"end":61},"obj":"Gene"},{"id":"1100","span":{"begin":66,"end":71},"obj":"Gene"}],"attributes":[{"id":"A1099","pred":"tao:has_database_id","subj":"1099","obj":"Gene:59272"},{"id":"A1100","pred":"tao:has_database_id","subj":"1100","obj":"Gene:1803"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T226","span":{"begin":29,"end":36},"obj":"Body_part"},{"id":"T227","span":{"begin":86,"end":94},"obj":"Body_part"},{"id":"T228","span":{"begin":190,"end":193},"obj":"Body_part"},{"id":"T229","span":{"begin":194,"end":202},"obj":"Body_part"}],"attributes":[{"id":"A226","pred":"fma_id","subj":"T226","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A227","pred":"fma_id","subj":"T227","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A228","pred":"fma_id","subj":"T228","obj":"http://purl.org/sig/ont/fma/fma62872"},{"id":"A229","pred":"fma_id","subj":"T229","obj":"http://purl.org/sig/ont/fma/fma62871"}],"text":"After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T482","span":{"begin":81,"end":85},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T483","span":{"begin":107,"end":110},"obj":"http://purl.obolibrary.org/obo/CLO_0008693"},{"id":"T484","span":{"begin":107,"end":110},"obj":"http://purl.obolibrary.org/obo/CLO_0008770"},{"id":"T485","span":{"begin":185,"end":189},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"}],"text":"After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T153","span":{"begin":29,"end":36},"obj":"Chemical"}],"attributes":[{"id":"A153","pred":"chebi_id","subj":"T153","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C."}

{"project":"LitCovid-sentences","denotations":[{"id":"T163","span":{"begin":0,"end":256},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"After four washes, the bound protein was detected using hACE2- or hDPP4-specific goat antibody (0.5 μg/ml, R\u0026D system) for 2 h at 37 °C, followed by incubation with HRP-conjugated anti-goat IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37 °C."}