PMC:7091888 / 23694-24512 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"961","span":{"begin":0,"end":3},"obj":"Chemical"},{"id":"972","span":{"begin":401,"end":409},"obj":"Species"},{"id":"973","span":{"begin":581,"end":592},"obj":"Species"},{"id":"974","span":{"begin":444,"end":449},"obj":"Species"},{"id":"975","span":{"begin":503,"end":508},"obj":"Species"},{"id":"976","span":{"begin":631,"end":636},"obj":"Species"},{"id":"977","span":{"begin":69,"end":72},"obj":"Chemical"},{"id":"978","span":{"begin":165,"end":169},"obj":"Chemical"},{"id":"979","span":{"begin":170,"end":177},"obj":"Chemical"},{"id":"980","span":{"begin":178,"end":181},"obj":"Chemical"},{"id":"981","span":{"begin":204,"end":228},"obj":"Chemical"}],"attributes":[{"id":"A961","pred":"tao:has_database_id","subj":"961","obj":"MESH:D012967"},{"id":"A972","pred":"tao:has_database_id","subj":"972","obj":"Tax:694009"},{"id":"A973","pred":"tao:has_database_id","subj":"973","obj":"Tax:3704"},{"id":"A974","pred":"tao:has_database_id","subj":"974","obj":"Tax:10090"},{"id":"A975","pred":"tao:has_database_id","subj":"975","obj":"Tax:10090"},{"id":"A976","pred":"tao:has_database_id","subj":"976","obj":"Tax:10090"},{"id":"A977","pred":"tao:has_database_id","subj":"977","obj":"MESH:D012967"},{"id":"A979","pred":"tao:has_database_id","subj":"979","obj":"MESH:D005998"},{"id":"A980","pred":"tao:has_database_id","subj":"980","obj":"MESH:D012967"},{"id":"A981","pred":"tao:has_database_id","subj":"981","obj":"MESH:C004692"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"SDS-PAGE and Western blot\nThe purified RBD proteins were analyzed by SDS-PAGE and Western blot as previously described.27,29 Briefly, proteins were separated by 10% Tris-glycine SDS-PAGE and stained with Coomassie brilliant blue or transferred to nitrocellulose membranes. The blots were blocked with 5% fat-free milk in PBS containing 0.5% Tween-20 (PBST) for 2 h at 37 °C and further incubated with SARS-CoV RBD-specific polyclonal antibody (mouse sera, 1:3,000),30 or MERS-CoV RBD-specific antibody (mouse sera, 1:3,000),31 overnight at 4 °C. The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5,000, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature and then visualized with ECL Western blot substrate reagents and Amersham Hyperfilm (GE Healthcare)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T189","span":{"begin":43,"end":51},"obj":"Body_part"},{"id":"T190","span":{"begin":134,"end":142},"obj":"Body_part"},{"id":"T191","span":{"begin":170,"end":177},"obj":"Body_part"},{"id":"T192","span":{"begin":313,"end":317},"obj":"Body_part"},{"id":"T193","span":{"begin":434,"end":442},"obj":"Body_part"},{"id":"T194","span":{"begin":493,"end":501},"obj":"Body_part"},{"id":"T195","span":{"begin":637,"end":640},"obj":"Body_part"}],"attributes":[{"id":"A189","pred":"fma_id","subj":"T189","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A190","pred":"fma_id","subj":"T190","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A191","pred":"fma_id","subj":"T191","obj":"http://purl.org/sig/ont/fma/fma82753"},{"id":"A192","pred":"fma_id","subj":"T192","obj":"http://purl.org/sig/ont/fma/fma62100"},{"id":"A193","pred":"fma_id","subj":"T193","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A194","pred":"fma_id","subj":"T194","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A195","pred":"fma_id","subj":"T195","obj":"http://purl.org/sig/ont/fma/fma62872"}],"text":"SDS-PAGE and Western blot\nThe purified RBD proteins were analyzed by SDS-PAGE and Western blot as previously described.27,29 Briefly, proteins were separated by 10% Tris-glycine SDS-PAGE and stained with Coomassie brilliant blue or transferred to nitrocellulose membranes. The blots were blocked with 5% fat-free milk in PBS containing 0.5% Tween-20 (PBST) for 2 h at 37 °C and further incubated with SARS-CoV RBD-specific polyclonal antibody (mouse sera, 1:3,000),30 or MERS-CoV RBD-specific antibody (mouse sera, 1:3,000),31 overnight at 4 °C. The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5,000, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature and then visualized with ECL Western blot substrate reagents and Amersham Hyperfilm (GE Healthcare)."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T3","span":{"begin":313,"end":317},"obj":"Body_part"}],"attributes":[{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0001913"}],"text":"SDS-PAGE and Western blot\nThe purified RBD proteins were analyzed by SDS-PAGE and Western blot as previously described.27,29 Briefly, proteins were separated by 10% Tris-glycine SDS-PAGE and stained with Coomassie brilliant blue or transferred to nitrocellulose membranes. The blots were blocked with 5% fat-free milk in PBS containing 0.5% Tween-20 (PBST) for 2 h at 37 °C and further incubated with SARS-CoV RBD-specific polyclonal antibody (mouse sera, 1:3,000),30 or MERS-CoV RBD-specific antibody (mouse sera, 1:3,000),31 overnight at 4 °C. The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5,000, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature and then visualized with ECL Western blot substrate reagents and Amersham Hyperfilm (GE Healthcare)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T446","span":{"begin":401,"end":409},"obj":"Disease"},{"id":"T447","span":{"begin":401,"end":405},"obj":"Disease"}],"attributes":[{"id":"A446","pred":"mondo_id","subj":"T446","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A447","pred":"mondo_id","subj":"T447","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"SDS-PAGE and Western blot\nThe purified RBD proteins were analyzed by SDS-PAGE and Western blot as previously described.27,29 Briefly, proteins were separated by 10% Tris-glycine SDS-PAGE and stained with Coomassie brilliant blue or transferred to nitrocellulose membranes. The blots were blocked with 5% fat-free milk in PBS containing 0.5% Tween-20 (PBST) for 2 h at 37 °C and further incubated with SARS-CoV RBD-specific polyclonal antibody (mouse sera, 1:3,000),30 or MERS-CoV RBD-specific antibody (mouse sera, 1:3,000),31 overnight at 4 °C. The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5,000, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature and then visualized with ECL Western blot substrate reagents and Amersham Hyperfilm (GE Healthcare)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T406","span":{"begin":262,"end":271},"obj":"http://purl.obolibrary.org/obo/UBERON_0000158"},{"id":"T407","span":{"begin":304,"end":307},"obj":"http://purl.obolibrary.org/obo/UBERON_0001013"},{"id":"T408","span":{"begin":444,"end":449},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"},{"id":"T409","span":{"begin":503,"end":508},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"},{"id":"T410","span":{"begin":540,"end":544},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T411","span":{"begin":621,"end":625},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T412","span":{"begin":631,"end":636},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"}],"text":"SDS-PAGE and Western blot\nThe purified RBD proteins were analyzed by SDS-PAGE and Western blot as previously described.27,29 Briefly, proteins were separated by 10% Tris-glycine SDS-PAGE and stained with Coomassie brilliant blue or transferred to nitrocellulose membranes. The blots were blocked with 5% fat-free milk in PBS containing 0.5% Tween-20 (PBST) for 2 h at 37 °C and further incubated with SARS-CoV RBD-specific polyclonal antibody (mouse sera, 1:3,000),30 or MERS-CoV RBD-specific antibody (mouse sera, 1:3,000),31 overnight at 4 °C. The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5,000, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature and then visualized with ECL Western blot substrate reagents and Amersham Hyperfilm (GE Healthcare)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T109","span":{"begin":0,"end":3},"obj":"Chemical"},{"id":"T110","span":{"begin":43,"end":51},"obj":"Chemical"},{"id":"T111","span":{"begin":69,"end":72},"obj":"Chemical"},{"id":"T112","span":{"begin":134,"end":142},"obj":"Chemical"},{"id":"T113","span":{"begin":165,"end":169},"obj":"Chemical"},{"id":"T114","span":{"begin":170,"end":177},"obj":"Chemical"},{"id":"T117","span":{"begin":178,"end":181},"obj":"Chemical"},{"id":"T118","span":{"begin":247,"end":261},"obj":"Chemical"},{"id":"T119","span":{"begin":686,"end":688},"obj":"Chemical"},{"id":"T122","span":{"begin":770,"end":778},"obj":"Chemical"},{"id":"T123","span":{"begin":803,"end":805},"obj":"Chemical"}],"attributes":[{"id":"A109","pred":"chebi_id","subj":"T109","obj":"http://purl.obolibrary.org/obo/CHEBI_8984"},{"id":"A110","pred":"chebi_id","subj":"T110","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A111","pred":"chebi_id","subj":"T111","obj":"http://purl.obolibrary.org/obo/CHEBI_8984"},{"id":"A112","pred":"chebi_id","subj":"T112","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A113","pred":"chebi_id","subj":"T113","obj":"http://purl.obolibrary.org/obo/CHEBI_9754"},{"id":"A114","pred":"chebi_id","subj":"T114","obj":"http://purl.obolibrary.org/obo/CHEBI_15428"},{"id":"A115","pred":"chebi_id","subj":"T114","obj":"http://purl.obolibrary.org/obo/CHEBI_29947"},{"id":"A116","pred":"chebi_id","subj":"T114","obj":"http://purl.obolibrary.org/obo/CHEBI_57305"},{"id":"A117","pred":"chebi_id","subj":"T117","obj":"http://purl.obolibrary.org/obo/CHEBI_8984"},{"id":"A118","pred":"chebi_id","subj":"T118","obj":"http://purl.obolibrary.org/obo/CHEBI_53325"},{"id":"A119","pred":"chebi_id","subj":"T119","obj":"http://purl.obolibrary.org/obo/CHEBI_474859"},{"id":"A120","pred":"chebi_id","subj":"T119","obj":"http://purl.obolibrary.org/obo/CHEBI_73610"},{"id":"A121","pred":"chebi_id","subj":"T119","obj":"http://purl.obolibrary.org/obo/CHEBI_90325"},{"id":"A122","pred":"chebi_id","subj":"T122","obj":"http://purl.obolibrary.org/obo/CHEBI_33893"},{"id":"A123","pred":"chebi_id","subj":"T123","obj":"http://purl.obolibrary.org/obo/CHEBI_73801"}],"text":"SDS-PAGE and Western blot\nThe purified RBD proteins were analyzed by SDS-PAGE and Western blot as previously described.27,29 Briefly, proteins were separated by 10% Tris-glycine SDS-PAGE and stained with Coomassie brilliant blue or transferred to nitrocellulose membranes. The blots were blocked with 5% fat-free milk in PBS containing 0.5% Tween-20 (PBST) for 2 h at 37 °C and further incubated with SARS-CoV RBD-specific polyclonal antibody (mouse sera, 1:3,000),30 or MERS-CoV RBD-specific antibody (mouse sera, 1:3,000),31 overnight at 4 °C. The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5,000, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature and then visualized with ECL Western blot substrate reagents and Amersham Hyperfilm (GE Healthcare)."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T3","span":{"begin":581,"end":609},"obj":"http://purl.obolibrary.org/obo/GO_0004601"}],"text":"SDS-PAGE and Western blot\nThe purified RBD proteins were analyzed by SDS-PAGE and Western blot as previously described.27,29 Briefly, proteins were separated by 10% Tris-glycine SDS-PAGE and stained with Coomassie brilliant blue or transferred to nitrocellulose membranes. The blots were blocked with 5% fat-free milk in PBS containing 0.5% Tween-20 (PBST) for 2 h at 37 °C and further incubated with SARS-CoV RBD-specific polyclonal antibody (mouse sera, 1:3,000),30 or MERS-CoV RBD-specific antibody (mouse sera, 1:3,000),31 overnight at 4 °C. The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5,000, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature and then visualized with ECL Western blot substrate reagents and Amersham Hyperfilm (GE Healthcare)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T139","span":{"begin":0,"end":25},"obj":"Sentence"},{"id":"T140","span":{"begin":26,"end":272},"obj":"Sentence"},{"id":"T141","span":{"begin":273,"end":545},"obj":"Sentence"},{"id":"T142","span":{"begin":546,"end":818},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"SDS-PAGE and Western blot\nThe purified RBD proteins were analyzed by SDS-PAGE and Western blot as previously described.27,29 Briefly, proteins were separated by 10% Tris-glycine SDS-PAGE and stained with Coomassie brilliant blue or transferred to nitrocellulose membranes. The blots were blocked with 5% fat-free milk in PBS containing 0.5% Tween-20 (PBST) for 2 h at 37 °C and further incubated with SARS-CoV RBD-specific polyclonal antibody (mouse sera, 1:3,000),30 or MERS-CoV RBD-specific antibody (mouse sera, 1:3,000),31 overnight at 4 °C. The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5,000, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature and then visualized with ECL Western blot substrate reagents and Amersham Hyperfilm (GE Healthcare)."}

{"project":"2_test","denotations":[{"id":"32203189-19683779-19774539","span":{"begin":122,"end":124},"obj":"19683779"},{"id":"32203189-20374001-19774540","span":{"begin":465,"end":467},"obj":"20374001"},{"id":"32203189-27750111-19774541","span":{"begin":524,"end":526},"obj":"27750111"}],"text":"SDS-PAGE and Western blot\nThe purified RBD proteins were analyzed by SDS-PAGE and Western blot as previously described.27,29 Briefly, proteins were separated by 10% Tris-glycine SDS-PAGE and stained with Coomassie brilliant blue or transferred to nitrocellulose membranes. The blots were blocked with 5% fat-free milk in PBS containing 0.5% Tween-20 (PBST) for 2 h at 37 °C and further incubated with SARS-CoV RBD-specific polyclonal antibody (mouse sera, 1:3,000),30 or MERS-CoV RBD-specific antibody (mouse sera, 1:3,000),31 overnight at 4 °C. The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5,000, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature and then visualized with ECL Western blot substrate reagents and Amersham Hyperfilm (GE Healthcare)."}