PMC:7091888 / 22811-23492 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"946","span":{"begin":387,"end":388},"obj":"Gene"},{"id":"947","span":{"begin":237,"end":238},"obj":"Gene"},{"id":"948","span":{"begin":78,"end":88},"obj":"Species"},{"id":"949","span":{"begin":90,"end":98},"obj":"Species"},{"id":"950","span":{"begin":104,"end":112},"obj":"Species"},{"id":"951","span":{"begin":226,"end":236},"obj":"Species"},{"id":"952","span":{"begin":268,"end":276},"obj":"Species"},{"id":"953","span":{"begin":311,"end":319},"obj":"Species"},{"id":"954","span":{"begin":376,"end":386},"obj":"Species"},{"id":"955","span":{"begin":437,"end":445},"obj":"Species"},{"id":"956","span":{"begin":499,"end":507},"obj":"Species"}],"attributes":[{"id":"A946","pred":"tao:has_database_id","subj":"946","obj":"Gene:43740568"},{"id":"A947","pred":"tao:has_database_id","subj":"947","obj":"Gene:43740568"},{"id":"A948","pred":"tao:has_database_id","subj":"948","obj":"Tax:2697049"},{"id":"A949","pred":"tao:has_database_id","subj":"949","obj":"Tax:694009"},{"id":"A950","pred":"tao:has_database_id","subj":"950","obj":"Tax:1335626"},{"id":"A951","pred":"tao:has_database_id","subj":"951","obj":"Tax:2697049"},{"id":"A952","pred":"tao:has_database_id","subj":"952","obj":"Tax:694009"},{"id":"A953","pred":"tao:has_database_id","subj":"953","obj":"Tax:1335626"},{"id":"A954","pred":"tao:has_database_id","subj":"954","obj":"Tax:2697049"},{"id":"A955","pred":"tao:has_database_id","subj":"955","obj":"Tax:694009"},{"id":"A956","pred":"tao:has_database_id","subj":"956","obj":"Tax:1335626"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"The construction, expression, and purification of recombinant RBD proteins of SARS-CoV-2, SARS-CoV, and MERS-CoV were performed as previously described with some modifications.27,28 Briefly, genes encoding residues 331-524 of SARS-CoV-2 S protein, residues 318-510 of SARS-CoV S protein, or residues 377-588 of MERS-CoV S proteins, were amplified by PCR using codon-optimized SARS-CoV-2 S protein (GenBank accession number: QHR63250.1), SARS-CoV S protein (GenBank accession number: AY278488.2), or MERS-CoV S protein (GenBank accession number: AFS88936.1), as respective template, and fused into pFUSE-hIgG1-Fc2 expression vector (hereinafter named hFc; InvivoGen, San Diego, CA)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T177","span":{"begin":66,"end":74},"obj":"Body_part"},{"id":"T178","span":{"begin":239,"end":246},"obj":"Body_part"},{"id":"T179","span":{"begin":279,"end":286},"obj":"Body_part"},{"id":"T180","span":{"begin":322,"end":330},"obj":"Body_part"},{"id":"T181","span":{"begin":389,"end":396},"obj":"Body_part"},{"id":"T182","span":{"begin":448,"end":455},"obj":"Body_part"},{"id":"T183","span":{"begin":510,"end":517},"obj":"Body_part"}],"attributes":[{"id":"A177","pred":"fma_id","subj":"T177","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A178","pred":"fma_id","subj":"T178","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A179","pred":"fma_id","subj":"T179","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A180","pred":"fma_id","subj":"T180","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A181","pred":"fma_id","subj":"T181","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A182","pred":"fma_id","subj":"T182","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A183","pred":"fma_id","subj":"T183","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"The construction, expression, and purification of recombinant RBD proteins of SARS-CoV-2, SARS-CoV, and MERS-CoV were performed as previously described with some modifications.27,28 Briefly, genes encoding residues 331-524 of SARS-CoV-2 S protein, residues 318-510 of SARS-CoV S protein, or residues 377-588 of MERS-CoV S proteins, were amplified by PCR using codon-optimized SARS-CoV-2 S protein (GenBank accession number: QHR63250.1), SARS-CoV S protein (GenBank accession number: AY278488.2), or MERS-CoV S protein (GenBank accession number: AFS88936.1), as respective template, and fused into pFUSE-hIgG1-Fc2 expression vector (hereinafter named hFc; InvivoGen, San Diego, CA)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T434","span":{"begin":78,"end":86},"obj":"Disease"},{"id":"T435","span":{"begin":78,"end":82},"obj":"Disease"},{"id":"T436","span":{"begin":90,"end":98},"obj":"Disease"},{"id":"T437","span":{"begin":90,"end":94},"obj":"Disease"},{"id":"T438","span":{"begin":226,"end":234},"obj":"Disease"},{"id":"T439","span":{"begin":226,"end":230},"obj":"Disease"},{"id":"T440","span":{"begin":268,"end":276},"obj":"Disease"},{"id":"T441","span":{"begin":268,"end":272},"obj":"Disease"},{"id":"T442","span":{"begin":376,"end":384},"obj":"Disease"},{"id":"T443","span":{"begin":376,"end":380},"obj":"Disease"},{"id":"T444","span":{"begin":437,"end":445},"obj":"Disease"},{"id":"T445","span":{"begin":437,"end":441},"obj":"Disease"}],"attributes":[{"id":"A434","pred":"mondo_id","subj":"T434","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A435","pred":"mondo_id","subj":"T435","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A436","pred":"mondo_id","subj":"T436","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A437","pred":"mondo_id","subj":"T437","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A438","pred":"mondo_id","subj":"T438","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A439","pred":"mondo_id","subj":"T439","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A440","pred":"mondo_id","subj":"T440","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A441","pred":"mondo_id","subj":"T441","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A442","pred":"mondo_id","subj":"T442","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A443","pred":"mondo_id","subj":"T443","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A444","pred":"mondo_id","subj":"T444","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A445","pred":"mondo_id","subj":"T445","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"The construction, expression, and purification of recombinant RBD proteins of SARS-CoV-2, SARS-CoV, and MERS-CoV were performed as previously described with some modifications.27,28 Briefly, genes encoding residues 331-524 of SARS-CoV-2 S protein, residues 318-510 of SARS-CoV S protein, or residues 377-588 of MERS-CoV S proteins, were amplified by PCR using codon-optimized SARS-CoV-2 S protein (GenBank accession number: QHR63250.1), SARS-CoV S protein (GenBank accession number: AY278488.2), or MERS-CoV S protein (GenBank accession number: AFS88936.1), as respective template, and fused into pFUSE-hIgG1-Fc2 expression vector (hereinafter named hFc; InvivoGen, San Diego, CA)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T395","span":{"begin":191,"end":196},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T396","span":{"begin":239,"end":256},"obj":"http://purl.obolibrary.org/obo/CHEBI_33708"},{"id":"T397","span":{"begin":279,"end":299},"obj":"http://purl.obolibrary.org/obo/CHEBI_33708"}],"text":"The construction, expression, and purification of recombinant RBD proteins of SARS-CoV-2, SARS-CoV, and MERS-CoV were performed as previously described with some modifications.27,28 Briefly, genes encoding residues 331-524 of SARS-CoV-2 S protein, residues 318-510 of SARS-CoV S protein, or residues 377-588 of MERS-CoV S proteins, were amplified by PCR using codon-optimized SARS-CoV-2 S protein (GenBank accession number: QHR63250.1), SARS-CoV S protein (GenBank accession number: AY278488.2), or MERS-CoV S protein (GenBank accession number: AFS88936.1), as respective template, and fused into pFUSE-hIgG1-Fc2 expression vector (hereinafter named hFc; InvivoGen, San Diego, CA)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T96","span":{"begin":66,"end":74},"obj":"Chemical"},{"id":"T97","span":{"begin":239,"end":246},"obj":"Chemical"},{"id":"T98","span":{"begin":279,"end":286},"obj":"Chemical"},{"id":"T99","span":{"begin":322,"end":330},"obj":"Chemical"},{"id":"T100","span":{"begin":389,"end":396},"obj":"Chemical"},{"id":"T101","span":{"begin":448,"end":455},"obj":"Chemical"},{"id":"T102","span":{"begin":510,"end":517},"obj":"Chemical"}],"attributes":[{"id":"A96","pred":"chebi_id","subj":"T96","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A97","pred":"chebi_id","subj":"T97","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A98","pred":"chebi_id","subj":"T98","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A99","pred":"chebi_id","subj":"T99","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A100","pred":"chebi_id","subj":"T100","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A101","pred":"chebi_id","subj":"T101","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A102","pred":"chebi_id","subj":"T102","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"The construction, expression, and purification of recombinant RBD proteins of SARS-CoV-2, SARS-CoV, and MERS-CoV were performed as previously described with some modifications.27,28 Briefly, genes encoding residues 331-524 of SARS-CoV-2 S protein, residues 318-510 of SARS-CoV S protein, or residues 377-588 of MERS-CoV S proteins, were amplified by PCR using codon-optimized SARS-CoV-2 S protein (GenBank accession number: QHR63250.1), SARS-CoV S protein (GenBank accession number: AY278488.2), or MERS-CoV S protein (GenBank accession number: AFS88936.1), as respective template, and fused into pFUSE-hIgG1-Fc2 expression vector (hereinafter named hFc; InvivoGen, San Diego, CA)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T137","span":{"begin":0,"end":681},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The construction, expression, and purification of recombinant RBD proteins of SARS-CoV-2, SARS-CoV, and MERS-CoV were performed as previously described with some modifications.27,28 Briefly, genes encoding residues 331-524 of SARS-CoV-2 S protein, residues 318-510 of SARS-CoV S protein, or residues 377-588 of MERS-CoV S proteins, were amplified by PCR using codon-optimized SARS-CoV-2 S protein (GenBank accession number: QHR63250.1), SARS-CoV S protein (GenBank accession number: AY278488.2), or MERS-CoV S protein (GenBank accession number: AFS88936.1), as respective template, and fused into pFUSE-hIgG1-Fc2 expression vector (hereinafter named hFc; InvivoGen, San Diego, CA)."}

{"project":"2_test","denotations":[{"id":"32203189-25240756-19774538","span":{"begin":179,"end":181},"obj":"25240756"}],"text":"The construction, expression, and purification of recombinant RBD proteins of SARS-CoV-2, SARS-CoV, and MERS-CoV were performed as previously described with some modifications.27,28 Briefly, genes encoding residues 331-524 of SARS-CoV-2 S protein, residues 318-510 of SARS-CoV S protein, or residues 377-588 of MERS-CoV S proteins, were amplified by PCR using codon-optimized SARS-CoV-2 S protein (GenBank accession number: QHR63250.1), SARS-CoV S protein (GenBank accession number: AY278488.2), or MERS-CoV S protein (GenBank accession number: AFS88936.1), as respective template, and fused into pFUSE-hIgG1-Fc2 expression vector (hereinafter named hFc; InvivoGen, San Diego, CA)."}