PMC:7075884 / 38459-38646 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T306","span":{"begin":106,"end":110},"obj":"Body_part"}],"attributes":[{"id":"A306","pred":"fma_id","subj":"T306","obj":"http://purl.org/sig/ont/fma/fma74402"}],"text":"Quantitative real-time PCR (qRT-PCR) reactions were performed in a 20 μl solution containing 10 μl TaqMan Gene Expression Master Mix (Life Technologies), 500 nM primers, and 300 nM probe."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T576","span":{"begin":65,"end":69},"obj":"http://purl.obolibrary.org/obo/CLO_0001564"},{"id":"T577","span":{"begin":65,"end":69},"obj":"http://purl.obolibrary.org/obo/CLO_0050194"},{"id":"T578","span":{"begin":106,"end":110},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"}],"text":"Quantitative real-time PCR (qRT-PCR) reactions were performed in a 20 μl solution containing 10 μl TaqMan Gene Expression Master Mix (Life Technologies), 500 nM primers, and 300 nM probe."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T877","span":{"begin":73,"end":81},"obj":"Chemical"},{"id":"T878","span":{"begin":181,"end":186},"obj":"Chemical"}],"attributes":[{"id":"A877","pred":"chebi_id","subj":"T877","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A878","pred":"chebi_id","subj":"T878","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"}],"text":"Quantitative real-time PCR (qRT-PCR) reactions were performed in a 20 μl solution containing 10 μl TaqMan Gene Expression Master Mix (Life Technologies), 500 nM primers, and 300 nM probe."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T147","span":{"begin":106,"end":121},"obj":"http://purl.obolibrary.org/obo/GO_0010467"}],"text":"Quantitative real-time PCR (qRT-PCR) reactions were performed in a 20 μl solution containing 10 μl TaqMan Gene Expression Master Mix (Life Technologies), 500 nM primers, and 300 nM probe."}

{"project":"LitCovid-sentences","denotations":[{"id":"T281","span":{"begin":0,"end":187},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Quantitative real-time PCR (qRT-PCR) reactions were performed in a 20 μl solution containing 10 μl TaqMan Gene Expression Master Mix (Life Technologies), 500 nM primers, and 300 nM probe."}