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PMC:7075884 / 34760-35985 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
1197 145-155 Chemical denotes penicillin MESH:D010406
1198 156-168 Chemical denotes streptomycin MESH:D013307
1199 292-295 Chemical denotes CO2 MESH:D002245
1200 428-432 Chemical denotes DPBS MESH:C012939
1201 448-452 Chemical denotes DMEM
1202 43-51 Disease denotes infected MESH:D007239
1203 198-207 Disease denotes infection MESH:D007239

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T274 15-20 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T275 226-230 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T276 305-310 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T277 376-380 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T278 398-403 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T279 485-490 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T280 540-545 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T281 719-723 Body_part denotes milk http://purl.org/sig/ont/fma/fma62100
T282 739-744 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T283 850-855 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T284 896-900 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T12 719-723 Body_part denotes milk http://purl.obolibrary.org/obo/UBERON_0001913
T13 773-777 Body_part denotes tube http://purl.obolibrary.org/obo/UBERON_0000025

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T83 198-207 Disease denotes infection http://purl.obolibrary.org/obo/MONDO_0005550
T84 711-718 Disease denotes sterile http://purl.obolibrary.org/obo/MONDO_0005047
T85 1120-1127 Disease denotes sterile http://purl.obolibrary.org/obo/MONDO_0005047

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T526 10-14 http://purl.obolibrary.org/obo/CLO_0009524 denotes Vero
T527 10-14 http://purl.obolibrary.org/obo/CLO_0050515 denotes Vero
T528 15-20 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T529 180-181 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T530 226-230 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T531 305-310 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T532 325-330 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T533 376-380 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T534 393-397 http://purl.obolibrary.org/obo/CLO_0009524 denotes Vero
T535 393-397 http://purl.obolibrary.org/obo/CLO_0050515 denotes Vero
T536 398-403 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T537 485-490 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T538 540-545 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T539 739-744 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T540 765-769 http://purl.obolibrary.org/obo/CLO_0001557 denotes a 15
T541 773-777 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube
T542 803-808 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T543 845-849 http://purl.obolibrary.org/obo/CLO_0009524 denotes Vero
T544 845-849 http://purl.obolibrary.org/obo/CLO_0050515 denotes Vero
T545 850-855 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T546 896-900 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T547 977-978 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T548 1075-1080 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T842 145-155 Chemical denotes penicillin http://purl.obolibrary.org/obo/CHEBI_17334|http://purl.obolibrary.org/obo/CHEBI_51356
T844 156-168 Chemical denotes streptomycin http://purl.obolibrary.org/obo/CHEBI_17076|http://purl.obolibrary.org/obo/CHEBI_58007
T846 292-295 Chemical denotes CO2 http://purl.obolibrary.org/obo/CHEBI_16526

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T255 0-331 Sentence denotes Confluent Vero cells in 75-cm2 flasks were infected with HSV in 3 ml of 199V (Gibco; 199 medium supplemented with 1% heat-inactivated FBS and 1% penicillin/streptomycin) medium at a multiplicity of infection (MOI) of 0.01 pfu/cell and incubated by gentle shaking for 2 hours at 37 °C with 5% CO2 to allow cells to absorb the virus.
T256 332-433 Sentence denotes After 2 hours, 199 V was aspirated from the cell culture and Vero cells were gently washed with DPBS.
T257 434-578 Sentence denotes 3 ml of fresh DMEM/5% FBS medium was added and the cells were incubated for 2 to 3 days until 100% of the cells display cytopathic effect (CPE).
T258 579-734 Sentence denotes When 100% CPE was observed, the flask was placed at −80 °C for 15 min, then allowed to warm up at room temperature and 3 ml of cold sterile milk was added.
T259 735-794 Sentence denotes The cells were collected into a 15 ml tube and kept on ice.
T260 795-1070 Sentence denotes To free virus particles from the cellular debris, Vero cells were sonicated three times (letting the cell suspension cool on ice for 1 min between sonications), for 30 seconds using a Branson Sonifier with microtip at an output setting of 4 (approximate Power Output of 15%).
T261 1071-1177 Sentence denotes The virus stock was aliquoted and transferred to sterile, screw-capped cryovials and was stored at −80 °C.
T262 1178-1225 Sentence denotes The viral titer was determined by plaque assay.