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PMC:7075884 / 34736-35985 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
1197 169-179 Chemical denotes penicillin MESH:D010406
1198 180-192 Chemical denotes streptomycin MESH:D013307
1199 316-319 Chemical denotes CO2 MESH:D002245
1200 452-456 Chemical denotes DPBS MESH:C012939
1201 472-476 Chemical denotes DMEM
1202 67-75 Disease denotes infected MESH:D007239
1203 222-231 Disease denotes infection MESH:D007239

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T274 39-44 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T275 250-254 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T276 329-334 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T277 400-404 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T278 422-427 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T279 509-514 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T280 564-569 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T281 743-747 Body_part denotes milk http://purl.org/sig/ont/fma/fma62100
T282 763-768 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T283 874-879 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T284 920-924 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T12 743-747 Body_part denotes milk http://purl.obolibrary.org/obo/UBERON_0001913
T13 797-801 Body_part denotes tube http://purl.obolibrary.org/obo/UBERON_0000025

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T83 222-231 Disease denotes infection http://purl.obolibrary.org/obo/MONDO_0005550
T84 735-742 Disease denotes sterile http://purl.obolibrary.org/obo/MONDO_0005047
T85 1144-1151 Disease denotes sterile http://purl.obolibrary.org/obo/MONDO_0005047

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T526 34-38 http://purl.obolibrary.org/obo/CLO_0009524 denotes Vero
T527 34-38 http://purl.obolibrary.org/obo/CLO_0050515 denotes Vero
T528 39-44 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T529 204-205 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T530 250-254 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T531 329-334 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T532 349-354 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T533 400-404 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T534 417-421 http://purl.obolibrary.org/obo/CLO_0009524 denotes Vero
T535 417-421 http://purl.obolibrary.org/obo/CLO_0050515 denotes Vero
T536 422-427 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T537 509-514 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T538 564-569 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T539 763-768 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T540 789-793 http://purl.obolibrary.org/obo/CLO_0001557 denotes a 15
T541 797-801 http://purl.obolibrary.org/obo/UBERON_0000025 denotes tube
T542 827-832 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T543 869-873 http://purl.obolibrary.org/obo/CLO_0009524 denotes Vero
T544 869-873 http://purl.obolibrary.org/obo/CLO_0050515 denotes Vero
T545 874-879 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T546 920-924 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T547 1001-1002 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T548 1099-1104 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T842 169-179 Chemical denotes penicillin http://purl.obolibrary.org/obo/CHEBI_17334|http://purl.obolibrary.org/obo/CHEBI_51356
T844 180-192 Chemical denotes streptomycin http://purl.obolibrary.org/obo/CHEBI_17076|http://purl.obolibrary.org/obo/CHEBI_58007
T846 316-319 Chemical denotes CO2 http://purl.obolibrary.org/obo/CHEBI_16526

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T254 0-23 Sentence denotes Viral stock preparation
T255 24-355 Sentence denotes Confluent Vero cells in 75-cm2 flasks were infected with HSV in 3 ml of 199V (Gibco; 199 medium supplemented with 1% heat-inactivated FBS and 1% penicillin/streptomycin) medium at a multiplicity of infection (MOI) of 0.01 pfu/cell and incubated by gentle shaking for 2 hours at 37 °C with 5% CO2 to allow cells to absorb the virus.
T256 356-457 Sentence denotes After 2 hours, 199 V was aspirated from the cell culture and Vero cells were gently washed with DPBS.
T257 458-602 Sentence denotes 3 ml of fresh DMEM/5% FBS medium was added and the cells were incubated for 2 to 3 days until 100% of the cells display cytopathic effect (CPE).
T258 603-758 Sentence denotes When 100% CPE was observed, the flask was placed at −80 °C for 15 min, then allowed to warm up at room temperature and 3 ml of cold sterile milk was added.
T259 759-818 Sentence denotes The cells were collected into a 15 ml tube and kept on ice.
T260 819-1094 Sentence denotes To free virus particles from the cellular debris, Vero cells were sonicated three times (letting the cell suspension cool on ice for 1 min between sonications), for 30 seconds using a Branson Sonifier with microtip at an output setting of 4 (approximate Power Output of 15%).
T261 1095-1201 Sentence denotes The virus stock was aliquoted and transferred to sterile, screw-capped cryovials and was stored at −80 °C.
T262 1202-1249 Sentence denotes The viral titer was determined by plaque assay.