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PMC:7075884 / 33203-34387 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
1175 534-538 Gene denotes US11 Gene:2703439
1176 764-771 Gene denotes β-actin Gene:728378
1177 4-9 Species denotes HSV-1 Tax:10298
1178 928-939 Species denotes horseradish Tax:3704
1179 108-111 Chemical denotes PBS MESH:D007854
1180 357-371 Chemical denotes polyacrylamide MESH:C016679
1181 450-459 Chemical denotes Tween PBS
1182 987-1044 Chemical denotes 5-bromo-4-chloro-3-indolylphosphate–nitroblue tetrazolium
1183 562-568 Disease denotes Cancer MESH:D009369

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T261 11-16 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T262 231-243 Body_part denotes g of protein http://purl.org/sig/ont/fma/fma61788
T263 236-243 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T264 290-298 Body_part denotes Proteins http://purl.org/sig/ont/fma/fma67257
T265 522-530 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T266 605-613 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T267 680-688 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T268 752-760 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T269 766-771 Body_part denotes actin http://purl.org/sig/ont/fma/fma67843
T270 871-879 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T271 952-959 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T82 562-568 Disease denotes Cancer http://purl.obolibrary.org/obo/MONDO_0004992

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T510 8-16 http://purl.obolibrary.org/obo/CLO_0001757 denotes 1, cells
T511 505-510 http://purl.obolibrary.org/obo/CLO_0007836 denotes mouse
T512 588-593 http://purl.obolibrary.org/obo/CLO_0007836 denotes mouse
T513 636-637 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T514 663-668 http://purl.obolibrary.org/obo/CLO_0007836 denotes mouse
T515 710-711 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T516 735-740 http://purl.obolibrary.org/obo/CLO_0007836 denotes mouse
T517 792-793 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T518 812-821 http://purl.obolibrary.org/obo/UBERON_0000158 denotes Membranes
T519 901-921 http://purl.obolibrary.org/obo/PR_000023540 denotes alkaline phosphatase

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T825 144-150 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T826 155-174 Chemical denotes protease inhibitors http://purl.obolibrary.org/obo/CHEBI_37670|http://purl.obolibrary.org/obo/CHEBI_60258
T828 164-174 Chemical denotes inhibitors http://purl.obolibrary.org/obo/CHEBI_35222
T829 236-243 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T830 357-371 Chemical denotes polyacrylamide http://purl.obolibrary.org/obo/CHEBI_51135|http://purl.obolibrary.org/obo/CHEBI_53656|http://purl.obolibrary.org/obo/CHEBI_60766
T833 406-420 Chemical denotes nitrocellulose http://purl.obolibrary.org/obo/CHEBI_53325
T834 952-959 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T835 989-994 Chemical denotes bromo http://purl.obolibrary.org/obo/CHEBI_22927|http://purl.obolibrary.org/obo/CHEBI_47265
T837 997-1003 Chemical denotes chloro http://purl.obolibrary.org/obo/CHEBI_47853
T838 1108-1116 Chemical denotes reagents http://purl.obolibrary.org/obo/CHEBI_33893

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T141 138-143 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T142 910-921 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T241 0-212 Sentence denotes For HSV-1, cells were harvested at the indicated times, collected by low-speed centrifugation and rinsed in PBS, then resuspended in RIPA lysis buffer and protease inhibitors (Complete Protease Inhibitor; Roche).
T242 213-289 Sentence denotes Approximately 60 μg of protein per sample was subjected to further analysis.
T243 290-500 Sentence denotes Proteins were electrophoretically separated on 8 to 10% denaturing polyacrylamide gels, electrically transferred to nitrocellulose sheets, blocked by 5% BSA in Tween PBS and reacted with the primary antibodies.
T244 501-658 Sentence denotes The mouse monoclonal antibody to US11 (Goodwin Institute for Cancer Research), and the mouse monoclonal antibody to ICP8, were used at a dilution of 1:1,000.
T245 659-730 Sentence denotes The mouse monoclonal antibody to ICP27 was used at a dilution of 1:250.
T246 731-811 Sentence denotes The mouse monoclonal antibody to β-actin (Sigma) was used at a 1:5,000 dilution.
T247 812-951 Sentence denotes Membranes were then reacted with the appropriate secondary antibody conjugated either to alkaline phosphatase or to horseradish peroxidase.
T248 952-1184 Sentence denotes Protein bands were visualized with 5-bromo-4-chloro-3-indolylphosphate–nitroblue tetrazolium (Denville Scientific, Inc.) or with ECL Western Blot detection reagents (Amersham Biosciences) according to the manufacturer’s instruction.

LitCovid-PD-HP

Id Subject Object Predicate Lexical cue hp_id
T8 562-568 Phenotype denotes Cancer http://purl.obolibrary.org/obo/HP_0002664