PMC:7075884 / 32213-32587 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"1143","span":{"begin":9,"end":17},"obj":"Disease"}],"attributes":[{"id":"A1143","pred":"tao:has_database_id","subj":"1143","obj":"MESH:D007239"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Briefly, infected Vero cells, 24 hpi were fixed and permeabilized using Cytofix/Cytoperm Solution Kit (BD Biosciences) according to the manufacturer’s instructions and stained with a mouse monoclonal antibody (mAb) specific for flavivirus group envelope proteins (1:250; EMD Millipore; clone D1–4G2-4-15) followed by incubation with an anti-mouse IgG - PE (1:1000 dilution)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T256","span":{"begin":23,"end":28},"obj":"Body_part"},{"id":"T257","span":{"begin":200,"end":208},"obj":"Body_part"},{"id":"T258","span":{"begin":254,"end":262},"obj":"Body_part"},{"id":"T259","span":{"begin":347,"end":350},"obj":"Body_part"}],"attributes":[{"id":"A256","pred":"fma_id","subj":"T256","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A257","pred":"fma_id","subj":"T257","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A258","pred":"fma_id","subj":"T258","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A259","pred":"fma_id","subj":"T259","obj":"http://purl.org/sig/ont/fma/fma62872"}],"text":"Briefly, infected Vero cells, 24 hpi were fixed and permeabilized using Cytofix/Cytoperm Solution Kit (BD Biosciences) according to the manufacturer’s instructions and stained with a mouse monoclonal antibody (mAb) specific for flavivirus group envelope proteins (1:250; EMD Millipore; clone D1–4G2-4-15) followed by incubation with an anti-mouse IgG - PE (1:1000 dilution)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T80","span":{"begin":103,"end":105},"obj":"Disease"}],"attributes":[{"id":"A80","pred":"mondo_id","subj":"T80","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"}],"text":"Briefly, infected Vero cells, 24 hpi were fixed and permeabilized using Cytofix/Cytoperm Solution Kit (BD Biosciences) according to the manufacturer’s instructions and stained with a mouse monoclonal antibody (mAb) specific for flavivirus group envelope proteins (1:250; EMD Millipore; clone D1–4G2-4-15) followed by incubation with an anti-mouse IgG - PE (1:1000 dilution)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T495","span":{"begin":18,"end":22},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T496","span":{"begin":18,"end":22},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T497","span":{"begin":23,"end":28},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T498","span":{"begin":181,"end":182},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T499","span":{"begin":183,"end":188},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"},{"id":"T500","span":{"begin":292,"end":303},"obj":"http://purl.obolibrary.org/obo/CLO_0002662"},{"id":"T501","span":{"begin":341,"end":346},"obj":"http://purl.obolibrary.org/obo/CLO_0007836"}],"text":"Briefly, infected Vero cells, 24 hpi were fixed and permeabilized using Cytofix/Cytoperm Solution Kit (BD Biosciences) according to the manufacturer’s instructions and stained with a mouse monoclonal antibody (mAb) specific for flavivirus group envelope proteins (1:250; EMD Millipore; clone D1–4G2-4-15) followed by incubation with an anti-mouse IgG - PE (1:1000 dilution)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T799","span":{"begin":239,"end":244},"obj":"Chemical"},{"id":"T800","span":{"begin":254,"end":262},"obj":"Chemical"},{"id":"T801","span":{"begin":353,"end":355},"obj":"Chemical"}],"attributes":[{"id":"A799","pred":"chebi_id","subj":"T799","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A800","pred":"chebi_id","subj":"T800","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A801","pred":"chebi_id","subj":"T801","obj":"http://purl.obolibrary.org/obo/CHEBI_16038"},{"id":"A802","pred":"chebi_id","subj":"T801","obj":"http://purl.obolibrary.org/obo/CHEBI_17553"},{"id":"A803","pred":"chebi_id","subj":"T801","obj":"http://purl.obolibrary.org/obo/CHEBI_74762"}],"text":"Briefly, infected Vero cells, 24 hpi were fixed and permeabilized using Cytofix/Cytoperm Solution Kit (BD Biosciences) according to the manufacturer’s instructions and stained with a mouse monoclonal antibody (mAb) specific for flavivirus group envelope proteins (1:250; EMD Millipore; clone D1–4G2-4-15) followed by incubation with an anti-mouse IgG - PE (1:1000 dilution)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T231","span":{"begin":0,"end":374},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Briefly, infected Vero cells, 24 hpi were fixed and permeabilized using Cytofix/Cytoperm Solution Kit (BD Biosciences) according to the manufacturer’s instructions and stained with a mouse monoclonal antibody (mAb) specific for flavivirus group envelope proteins (1:250; EMD Millipore; clone D1–4G2-4-15) followed by incubation with an anti-mouse IgG - PE (1:1000 dilution)."}